LOCUS       sequence-254067-       12783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating Tg mice expressing the 2P-activatable RAF system with
            P2A and full-length RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata 
            S, Matsuda M
  TITLE     FRET-assisted photoactivation of flavoproteins for in vivo 
            two-photon optogenetics.
  JOURNAL   Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5.
  PUBMED    31501546
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5."
COMMENT     Sequence Label: pT2ADW_2paRAF
FEATURES             Location/Qualifiers
     source          1..12783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5838..5894
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5895..6404
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             6423..7118
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6556..6574)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6824..6843
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7205..7793
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7258..7278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7676..7687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7858..7992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7882..7901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7936..7955
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8114..8276,8277..8288)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8318..8337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8319..8337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8347..8364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8347..8363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8356..8378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8505..8959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8591..8610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8801..8822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8985..9089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9090..9158,9159..9950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9308..9327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10121..10709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10610..10629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10863..10880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10997..11018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11033..11038,11039..11056,11057..11063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     11095..11111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11095..11111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11130..11150
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11132..11150
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(11207..11218,11219..11406)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggsgcgcgc crgggggggs gggggggggs grggggsggg
      541 gsggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc accatgaaga
     1741 tggacaagaa aaccatcgtg tggtttcgga gagatctgag gattgaggac aacccagccc
     1801 tggccgctgc cgctcacgaa ggcagcgtgt ttccagtgtt catctggtgc cccgaggaag
     1861 agggacagtt ctacccagga agggccagcc gctggtggat gaagcagtcc ctggctcacc
     1921 tgtctcagag tctgaaggcc ctggggtcag atctgaccct gatcaaaacc cataacacaa
     1981 tcagcgccat tctggactgt attcgggtga ctggagccac caaagtggtg tttaatcacc
     2041 tgtatgatcc cgtgagcctg gtgcgggacc atacagtgaa ggagaaactg gtggaaagag
     2101 gcatcagtgt gcagtcatac aacggggatc tgctgtatga gccatgggaa atctactgcg
     2161 agaaggggaa accctttacc agcttcaatt cctactggaa gaaatgtctg gacggatctg
     2221 gaatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     2281 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     2341 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     2401 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     2461 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     2521 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     2581 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     2641 gggaggcatt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     2701 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat
     2761 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     2821 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     2881 gatacggtag cggtccatgg aggctgatgc ctattacagc cgctgccgaa gctatctggg
     2941 cctgctccat tgaagagctg ggactggaaa acgaggccga aaagccttct aatgctctgc
     3001 tgactcgcgc ctggtcccca ggatggtcta acgctgataa actgctgaat gagttcatcg
     3061 aaaagcagct gattgactat gccaaaaact ctaagaaagt ggtgggcaac agcaccagcc
     3121 tgctgtctcc atacctgcac tttggagaga tcagtgtgcg gcacgtgttc cagtgcgcta
     3181 gaatgaagca gatcatttgg gccagggata aaaacagtga gggcgaagag tcagccgacc
     3241 tgtttctgag gggaatcgga ctgagggagt actcccgcta tatttgtttc aattttccct
     3301 tcactcacga acagtcactg ctgagccatc tgcggttctt tccttgggac gctgatgtgg
     3361 acaagttcaa agcctggcgg cagggaagaa caggctatcc cctggtggat gctggaatga
     3421 gagagctgtg ggccactggc tggatgcaca ataggatccg cgtgattgtg agctcctttg
     3481 ctgtgaagtt cctgctgctg ccttggaagt ggggcatgaa atatttttgg gatacactgc
     3541 tggatgccga cctggagtgt gacatcctgg ggtggcagta catctccgga tctattccag
     3601 atggccatga actggatagg ctggacaacc ccgctctgca gggcgctaag tatgaccctg
     3661 agggagaata catcaggcag tggctgcctg agctggctag gctgccaact gaatggattc
     3721 accatccctg ggacgctcct ctgacagtgc tgaaggccag tggggtggag ctgggaacta
     3781 attacgctaa acctatcgtg gatattgaca ccgccagaga gctgctggct aaggccatct
     3841 cccggaccag agaagcccag atcatgattg gcgctgccat ggagcacata cagggagctt
     3901 ggaagacgat cagcaatggt tttggattca aagatgccgt gtttgatggc tccagctgca
     3961 tctctcctac aatagttcag cagtttggct atcagcgccg ggcatcagat gatggcaaac
     4021 tcacagatcc ttctaagaca agcaacacta tccgtgtttt cttgccgaac aagcaaagaa
     4081 cagtggtcaa tgtgcgaaat ggaatgagct tgcatgactg ccttatgaaa gcactcaagg
     4141 tgaggggcct gcaaccagag tgctgtgcag tgttcagact tctccacgaa cacaaaggta
     4201 aaaaagcacg cttagattgg aatactgatg ctgcgtcttt gattggagaa gaacttcaag
     4261 tagatttcct ggatcatgtt cccctcacaa cacacaactt tgctcggaag acgttcctga
     4321 agcttgcctt ctgtgacatc tgtcagaaat tcctgctcaa tggatttcga tgtcagactt
     4381 gtggctacaa atttcatgag cactgtagca ccaaagtacc tactatgtgt gtggactgga
     4441 gtaacatcag acaactctta ttgtttccaa attccactat tggtgatagt ggagtcccag
     4501 cactaccttc tttgactatg cgtcgtatgc gagagtctgt ttccaggatg cctgttagtt
     4561 ctcagcacag atattctaca cctcacgcct tcacctttaa cacctccagt ccctcatctg
     4621 aaggttccct ctcccagagg cagaggtcga catccacacc taatgtccac atggtcagca
     4681 ccaccctgcc tgtggacagc aggatgattg aggatgcaat tcgaagtcac agcgaatcag
     4741 cctcaccttc agccctgtcc agtagcccca acaatctgag cccaacaggc tggtcacagc
     4801 cgaaaacccc cgtgccagca caaagagagc gggcaccagt atctgggacc caggagaaaa
     4861 acaaaattag gcctcgtgga cagagagatt caagctatta ttgggaaata gaagccagtg
     4921 aagtgatgct gtccactcgg attgggtcag gctcttttgg aactgtttat aagggtaaat
     4981 ggcacggaga tgttgcagta aagatcctaa aggttgtcga cccaacccca gagcaattcc
     5041 aggccttcag gaatgaggtg gctgttctgc gcaaaacacg gcatgtgaac attctgcttt
     5101 tcatggggta catgacaaag gacaacctgg caattgtgac ccagtggtgc gagggcagca
     5161 gcctctacaa acacctgcat gtccaggaga ccaagtttca gatgttccag ctaattgaca
     5221 ttgcccggca gacggctcag ggaatggact atttgcatgc aaagaacatc atccatagag
     5281 acatgaaatc caacaatata tttctccatg aaggcttaac agtgaaaatt ggagattttg
     5341 gtttggcaac agtaaagtca cgctggagtg gttctcagca ggttgaacaa cctactggct
     5401 ctgtcctctg gatggcccca gaggtgatcc gaatgcagga taacaaccca ttcagtttcc
     5461 agtcggatgt ctactcctat ggcatcgtat tgtatgaact gatgacgggg gagcttcctt
     5521 attctcacat caacaaccga gatcagatca tcttcatggt gggccgagga tatgcctccc
     5581 cagatcttag taagctatat aagaactgcc ccaaagcaat gaagaggctg gtagctgact
     5641 gtgtgaagaa agtaaaggaa gagaggcctc tttttcccca gatcctgtct tccattgagc
     5701 tgctccaaca ctctctaccg aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg
     5761 cagcccacac tgaggatatc aatgcttgca cgctgaccac gtccccgagg ctgcctgtct
     5821 tcctcgaggg aagcggagcg acgaatttta gtctactgaa acaagcggga gacgtggagg
     5881 aaaaccctgg acctatgaat ggagctatag gaggtgacct tttgctcaat tttcctgaca
     5941 tgtcggtcct agagcgccaa agggctcacc tcaagtacct caatcccacc tttgattctc
     6001 ctctcgccgg cttctttgcc gattcttcaa tgattaccgg cggcgagatg gacagctatc
     6061 tttcgactgc cggtttgaat cttccgatga tgtacggtga gacgacggtg gaaggtgatt
     6121 caagactctc aatttcgccg gaaacgacgc ttgggactgg aaatttcaag gcagcgaagt
     6181 ttgatacaga gactaaggat tgtaatgagg cggcgaagaa gatgacgatg aacagagatg
     6241 acctagtaga agaaggagaa gaagagaagt cgaaaataac agagcaaaac aatgggagca
     6301 caaaaagcat caagaagatg aaacacaaag ccaagaaaga agagaacaat ttctctaatg
     6361 attcatctaa agtgacgaag gaattggaga aaacggatta tattcatctc gagggatctg
     6421 gaatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg
     6481 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     6541 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg
     6601 acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca
     6661 tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6721 tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga
     6781 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga
     6841 agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg
     6901 gcgacattaa gatggccctg cgcctgaagg acggaggccg ctacctggcg gacttcaaga
     6961 ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt
     7021 tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg
     7081 gccgccactc caccggcggc atggacgagc tgtacaagtc tagaaagatg agcaaagatg
     7141 gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagtc gacggatcct
     7201 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7261 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7321 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7381 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7441 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7501 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7561 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7621 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7681 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7741 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatctcga
     7801 caaaggatcc aatggaggcc gccaccgcgg tggagctcga attaattcat cgatgatgat
     7861 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7921 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7981 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     8041 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     8101 ctagatcaga tctttcttgc ttttactttt acttccttaa tactcaagta caattttaat
     8161 ggagtacttt tttactttta ctcaagtaag attctagcca gatactttta cttttaattg
     8221 agtaaaattt tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta
     8281 cacctctgtc aagaaccata tgccggtacc caattcgccc tatagtgagt cgtattacgc
     8341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     8521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accaccacac ccgccgcgcc
     8581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8941 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9061 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9301 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9601 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10261 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10741 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10801 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10861 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10921 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10981 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11041 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgatcgg ataacaattt cacacaggaa
    11101 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    11161 tggagctcgg atctgctggg cttgctgaag gtagggggtc aagaaccaga ggtgtaaagt
    11221 acttgagtaa ttttacttga ttactgtact taagtattat ttttggggat ttttacttta
    11281 cttgagtaca attaaaaatc aatactttta cttttactta attacatttt tttagaaaaa
    11341 aaagtacttt ttactcctta caattttatt tacagtcaaa aagtacttat tttttggaga
    11401 tcacttgggc cctctagatg catgccggct ggcccaggcc atcggcattc cggagcccag
    11461 ggtccagatt tggtttcaga atgagaggtc acgccagctg aggcagcacc ggcgggaatc
    11521 tcggccctgg cccgggagac gcggcccgcc agaaggccgg cgaaagcgga ccgccgtcac
    11581 cggatcccag accgccctgc tcctccgagc ctttgagaag gatcgctttc caggcatcgc
    11641 cgcccgggag gagctggcca gagagacggg cctcccggag tccaggattc agatctggtt
    11701 tcagaatcga agggccaggc acccgggaca gggtggcagg gcgcccgcgc aggcaggcgg
    11761 cctgtgcagc gcggcccccg gcgggggtca ccctgctccc tcgtgggtcg ccttcgccca
    11821 caccggcgcg tggggaacgg ggcttcccgc accccacgtg ccctgcgcgc ctggggctct
    11881 cccacagggg gctttcgtga gccaggcagc gagggccgcc cccgcgctgc agcccagcca
    11941 ggccgcgccg gcagagggga tctcccaacc tgccccggcg cgcggggatt tcgcctacgc
    12001 cgccccggct cctccggacg gggcgctctc ccaccctcag gctcctcgct ggcctccgca
    12061 cccgggcaaa agccgggagg accgggaccc gcagcgcgac ggcctgccgg gcccctgcgc
    12121 ggtggcacag cctgggcccg ctcaagcggg gccgcagggc caaggggtgc ttgcgccacc
    12181 cacgtcccag gggagtccgt ggtggggctg gggccggggt ccccaggtcg ccggggcggc
    12241 gtgggaaccc caagccgggg cagctccacc tccccagccc gcgcccccgg acgcctccgc
    12301 ctccgcgcgg caggggcaga tgcaaggcat cccggcgccc tcccaggcgc tccaggagcc
    12361 ggcgccctgg tctgcactcc cctgcggcct gctgctggat gagctcctgg cgagcccgga
    12421 gtttctgcag caggcgcaac ctctcctaga aacggaggcc ccgggggagc tggaggcctc
    12481 ggaagaggcc gcctcgctgg aagcacccct cagcgaggaa gaataccggg ctctgctgga
    12541 ggagctttag gacgcggggt tgggacgggg tcgggtggtt cggggcaggg ccgtggcctc
    12601 tctttcgcgg ggaacacctg gctggctacg gaggggcgtg tctccgcccc gccccctcca
    12661 ccgggctgac cggcctggga ttcctgcctt ctaggtctag gcccggtgag agactccaca
    12721 ccgcggagaa ctgccattct ttcctgggca tcccggggat cccagagccg gcccagcatg
    12781 ctc
//
