LOCUS       sequence-254117-       11144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 36xMashTag with MoonTag frame as mainframe, followed by 
            BFP linker and 24 repeats of PP7 hairpins in 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: MStart-36xMashTag-BFP-3xStop-24xPP7
            (Moonstart...
FEATURES             Location/Qualifiers
     source          1..11144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1025..1081
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1083..1127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1130..1186
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1188..1232
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1235..1291
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1293..1337
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1340..1396
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1398..1442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1445..1501
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1503..1547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1550..1606
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1608..1652
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1655..1711
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1713..1757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1760..1816
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1818..1862
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1865..1921
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1970..2026
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2028..2072
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2075..2131
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2133..2177
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2180..2236
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2238..2282
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2285..2341
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2343..2387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2390..2446
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2448..2492
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2495..2551
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2553..2597
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2600..2656
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2658..2702
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2705..2761
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2763..2807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2810..2866
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2868..2912
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2915..2971
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2973..3017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3020..3076
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3078..3122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3125..3181
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3183..3227
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3230..3286
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3288..3332
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3335..3391
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3393..3437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3440..3496
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3498..3542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3581..3637
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3639..3683
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3686..3742
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3744..3788
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3791..3847
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3849..3893
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3896..3952
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3954..3998
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4001..4057
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4059..4103
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4106..4162
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4164..4208
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4211..4267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4269..4313
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4316..4372
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4374..4418
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4421..4477
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4479..4523
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4584..4628
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4689..4733
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4736..4792
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4794..4838
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(7152..7169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7158..7382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7428..7856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7515..7534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7725..7746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7865..7885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7870..8199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8050..8185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8112..8131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8247..8294
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8313..8687
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8817..8938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8854..8873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8908..8927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8987..9003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8987..9003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9000..9022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9011..9027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9035..9041,9042..9059,9060..9065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9080..9101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9218..9235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9389..9977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9469..9488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10148..10939,10940..11008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10771..10790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11009..11113)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaccgg
     1021 tcaagaagaa cttctcagca agaattatca tttggagaac gaagtggctc gtctcaagaa
     1081 acaagaacga gcaagaatta ctggaattgg acaagtgggc aagcctccag aagaactgct
     1141 ttcaaagaat taccacctgg aaaacgaggt tgccagactc aaaaagcaag aacgagcagg
     1201 aactcctcga actcgacaag tgggcttctc tccagaggag ttactcagca aaaattatca
     1261 tttggagaac gaagttgcac gactcaagaa agaaaaacga acaggagtta ctcgagctcg
     1321 acaagtgggc cagcctccag aggagttact ctcgaaaaat tatcatctcg aaaacgaagt
     1381 ggctcggctc aaaaagcaag aacgaacaag aactcctcga actggacaag tgggcatctc
     1441 tcgagaagag ctattatcca aaaactacca cctcgaaaac gaggtggcac gcctcaaaaa
     1501 gcaagaacga gcaagaattg ttggaactcg acaagtgggc aagccttcag aagagctgct
     1561 atccaagaat tatcatctcg agaacgaggt ggcgcgtctc aagaaacaag aacgagcaag
     1621 aattactcga actcgacaag tgggcttctc tcgagaggaa ctgctctcga agaactatca
     1681 tctggaaaac gaggtcgctc gactcaaaaa gcaaaaacga acaggagctg ctggagttgg
     1741 acaagtgggc cagcctccag aggaactact ttcaaagaat taccacctcg aaaacgaagt
     1801 tgctcgactc aaaaagcaag aacgaacagg aattactcga actcgacaag tgggcatctc
     1861 tccagaagaa ttactcagca aaaattatca tctggaaaac gaggtggcga gactcaaaaa
     1921 gcaagaacga gcaggaactc ctcgaattgg acaagtgggc aagcctgcag aagagttgct
     1981 atcgaaaaat tatcatctgg agaacgaagt tgcaaggctc aaaaaggaaa aacgagcaag
     2041 aactactcga actcgacaag tgggcttctc tgcagaggag ttgctctcga aaaactacca
     2101 cttggaaaac gaggtcgcga ggctcaaaaa gcaaaaacga acaagagctg ctcgagctgg
     2161 acaagtgggc cagcctggag aggagttact cagcaaaaac tatcatctcg agaacgaagt
     2221 cgcgcgcctc aagaaagaag aacgaacaag aactactcga actggacaag tgggcatctc
     2281 tgcagaagaa ctcttatcga agaactacca cctcgaaaac gaggtcgcca ggctcaaaaa
     2341 ggaagaacga gcaggaactg ctggagctgg acaagtgggc ctctcttcag aggaactcct
     2401 cagcaagaac taccacttgg agaacgaggt cgcgagactc aagaaagaaa aacgaacaag
     2461 agctactcga gctcgacaag tgggcctccc ttcagaggaa ttactcagca agaattatca
     2521 tttggagaac gaagtcgcca ggctcaagaa acaaaaacga acaggaatta ctcgagctgg
     2581 acaagtgggc aagcctggag aggaattact cagcaaaaac taccacttgg aaaacgaagt
     2641 cgccaggctc aaaaaagaaa aacgaacagg agttactgga gctcgacaag tgggcttctc
     2701 tgcagaagag ttactcagca aaaattacca cttggagaac gaagtggcaa ggctcaagaa
     2761 agaagaacga gcaggaactg ctggagctcg acaagtgggc ctctttgcgg aggagctctt
     2821 atcgaagaac taccacctcg agaacgaagt cgcccgcctc aagaaacaaa aacgaacagg
     2881 agctactcga gttggacaag tgggcctccc tggagaagag ctcctcagca agaactacca
     2941 cttggaaaac gaggtggcac gcctcaagaa acaaaaacga acaagaactc ctcgagttgg
     3001 acaagtgggc aagcctcgag aagagttact atccaagaat tatcatctcg agaacgaggt
     3061 ggctcgactc aaaaagcaaa aacgaacagg agctcctgga gctcgacaag tgggcttctc
     3121 tccagaggaa ctcctgtcga agaactatca tctcgaaaac gaggttgcaa gactcaaaaa
     3181 ggaagaacga gcaggaactc ctggagctcg acaagtgggc ctctctggag aggaactact
     3241 ctcaaagaat taccacctgg aaaacgaagt tgcacgtctc aagaaacaaa aacgaacagg
     3301 agttactgga gctcgacaag tgggcctccc tcgagaagaa ctgctctcaa aaaattatca
     3361 tttggaaaac gaggttgcac gtctcaaaaa ggaaaaacga acaagaatta ctcgagctcg
     3421 acaagtgggc aagccttcag aggaacttct gtcgaaaaat tatcatctcg agaacgaagt
     3481 tgcccgactc aaaaagaaaa aacgaacaag agttactgga gctggacaag tgggcttctc
     3541 tggggttcga agcaggggca cgcggtccgg ggttcgaagg gaggaacttc tcagcaagaa
     3601 ttatcatttg gagaacgaag tggctcgtct caagaaacaa aaacgaacag gaactcctgg
     3661 aattggacaa gtgggcaagc cttcagaaga actgctttca aagaattacc atctggaaaa
     3721 cgaggttgcc agactcaaga agcaagaacg agcaggaatt actcgaattg gacaagtggg
     3781 cctccctcca gaggagttgc tcagcaagaa ttatcatttg gagaacgaag ttgcacgact
     3841 caagaaagaa gaacgaacag gagttactcg agctcgacaa gtgggccagc ctccagagga
     3901 attactctcg aaaaattatc atctcgaaaa cgaagtggcc cggctcaaaa agcaagaacg
     3961 aacaggaact cctcgagctc gacaagtggg cctccctcga gaggaactat tatccaagaa
     4021 ctaccacctc gaaaacgagg tggcacgcct caagaagcaa aaacgaacag gaattgttgg
     4081 aactcgacaa gtgggcaagc cttcagagga actactatcc aaaaattatc atctcgagaa
     4141 cgaggtggcg cgtctcaaaa agcaaaaacg agcaggaact actggaactc gacaagtggg
     4201 cctccctcga gaggaattgc tctcgaagaa ctatcatctg gagaacgagg tcgctcgact
     4261 caagaagcaa gaacgaacag gagctgctgg aactggacaa gtgggccagc ctccagaaga
     4321 acttctttca aaaaattacc acctcgaaaa cgaagttgcg cgactcaaaa agcaaaaacg
     4381 agcaggagct cctcgaactc gacaagtggg catctctcca gaggagttac tcagcaagaa
     4441 ttatcatctg gaaaacgagg tggcgagact caaaaagcaa gaacgagcaa gagctcctcg
     4501 agctggacaa gtgggcatcc ctgcagaaga gttactcagg aaaaattatc atctggaaaa
     4561 cgaagttgca aggctcaaaa aggaagaacg aacaggagct actcgaactc gacaagtggg
     4621 catccctgca gaggagctgc tcaggaaaaa ctaccacttg gaaaacgagg tcgcgaggct
     4681 caagaagcaa gaacgagcag gaactgctcg agctggacaa gtgggccagc ctggagaaga
     4741 gcttctcagc aagaactatc atctcgagaa cgaagtcgcg cgcctcaaga aggaaaaacg
     4801 agcaagaact actcgagctc gacaagtggg caagcctgca gggcgtacgg ggaccggtgg
     4861 agcgagctca ttcgggagaa catgcacatc aagctataca tggagggcac cgtggacaac
     4921 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatc
     4981 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctacgagc
     5041 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
     5101 cagtccttcc ctcagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     5161 ctcaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     5221 agaggggtca acttcacatc caacggccct gtcatgcaga agaaaacact cggctgggag
     5281 gccttcaccg agacgctgta ccccgccgac ggcggcctgg aaggcagaaa cgacatggcc
     5341 ctcaagctcg tgggcgggag ccatctcatc gcaaacatca agaccacata cagatccaag
     5401 aaacccgcaa agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     5461 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     5521 tgcgacctcc caagcaaact ggggcacaaa ctcaatccgc gggggtgagt gagtgagggg
     5581 ctagcccggt tgtacaggta ccggatccta aggtacctaa ttgcctagaa aggagcagac
     5641 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     5701 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     5761 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     5821 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
     5881 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
     5941 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
     6001 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
     6061 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
     6121 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     6181 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
     6241 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
     6301 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
     6361 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
     6421 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
     6481 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
     6541 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
     6601 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
     6661 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     6721 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
     6781 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
     6841 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
     6901 gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga
     6961 aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata
     7021 tgggctcgct ggctgcagta ttcccgggtt cattagatct cgcgaagggc gaattcacga
     7081 ggcgcgcccg gatcgcgatc gcaatcttaa ttaactcgag tctagagggc ccgtttaaac
     7141 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     7201 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7261 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7321 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7381 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7861 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7921 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8041 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8101 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     8161 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     8221 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     8281 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     8341 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     8401 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8461 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     8521 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8581 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8641 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8701 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8761 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8821 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8881 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8941 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9061 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
