LOCUS       sequence-254278-        5816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination plasmid containing the 35S:XVE estradiol inducible 
            driver and the A-G overhangs for GreenGate cloning of an expression 
            casette for estradiole-inducible expression in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Denay G, Schultz P, Hansch S, Weidtkamp-Peters S, Simon R
  TITLE     Over the rainbow: A practical guide for fluorescent protein 
            selection in plant FRET experiments.
  JOURNAL   Plant Direct. 2019 Dec 6;3(12):e00189. doi: 10.1002/pld3.189. 
            eCollection 2019 Dec.
  PUBMED    31844834
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2019-12-6"; volume: "3(12):e00189. doi"; pages: " 
            10.1002/pld3.189. eCollection 2019 Dec"
COMMENT     Sequence Label: pGD283
FEATURES             Location/Qualifiers
     source          1..5816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    49..73
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(164..752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(244..263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(923..1711)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2002..2437
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2564..2586
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        3179..3524
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3467..3487
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    3552..3607
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3613..3864
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             3880..4113
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             4123..5058
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4171..5061
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      5118..5759
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
ORIGIN
        1 gtattctaga ccaagcatgc acagctggcg ttctcagcca gatccccttg acaggatata
       61 ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc
      121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg
      661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
      901 atgtgtaaca ttggtctagt gattatttgc cgactacctt ggtgatctcg cctttcacgt
      961 agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat
     1021 aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     1081 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     1141 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1201 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1261 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1321 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     1381 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1441 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     1501 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1561 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1621 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1681 tcgatacttc ggcgatcacc gcttccctca taacacccct tgtattactg tttatgtaag
     1741 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     1801 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     1861 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     1921 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     1981 atggggcgat tcaggcgatc cccatccaac agcccgccgt cgagcgggct tttttatccc
     2041 cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct
     2101 tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg
     2161 tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa
     2221 tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc aaacacccct
     2281 cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat tatgaatata
     2341 tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc
     2401 cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg
     2461 gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc
     2521 aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg atatattgtg
     2581 gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta attaaacacg
     2641 gcgcgccaac agcgatcgca ccaggtacca cctccgatcc attcggtccc cagattagcc
     2701 ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcacgtc
     2761 tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg
     2821 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     2881 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa
     2941 ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg
     3001 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca
     3061 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     3121 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     3181 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     3241 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     3301 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     3361 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     3421 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     3481 acccttcctc tatataagga agttcatttc atttggagag aacacggggg acgagctaac
     3541 atatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta
     3601 caattacagg ctatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt
     3661 gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg
     3721 ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt
     3781 gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg
     3841 ccgctggtag gtcgtgtggc tgccggtgaa ccgtcgagcg cccccccgac cgatgtcagc
     3901 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
     3961 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc
     4021 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt
     4081 accgatgccc ttggaattga cgagtacggt ggggatccgt ctgctggaga catgagagct
     4141 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
     4201 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
     4261 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
     4321 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
     4381 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg
     4441 attggactcg tctggcgctc catggagcac ccagggaagc tactgtttgc tcctaacttg
     4501 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
     4561 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
     4621 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
     4681 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
     4741 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
     4801 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
     4861 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg agatgctgga cgcccaccgc
     4921 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
     4981 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
     5041 gagggtttcc ctgccacagt ctcaggtgga tcctagataa cctttacctt cattccctgt
     5101 aaattggtac cctgcaaagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt
     5161 caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat
     5221 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
     5281 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
     5341 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
     5401 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg
     5461 gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt
     5521 attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca
     5581 agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc
     5641 cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag
     5701 tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac atgcatcaat
     5761 cgactatgag taggcaagat gttctgtagg accttgagac cgaaaaggtg gtctca
//
