LOCUS       sequence-254364-        4431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CmYLCV::Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_A5803
FEATURES             Location/Qualifiers
     source          1..4431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     291..310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     544..561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1074..2726
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1140..1158)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2736..2984
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3061..3165
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(4233..4431,1..390)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
       61 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      121 acggggggtt cgtgctcaca gcccagcttg gagcgaacga cctacaccga actgagatac
      181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      421 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      481 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      541 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccac ctgctcatga tcggcgcgcc
      601 tggcagacat actgtcccac aaatgaagat ggaatctgta aaagaaaacg cgtgaaataa
      661 tgcgtctgac aaaggttagg tcggctgcct ttaatcaata ccaaagtggt ccctaccacg
      721 atggaaaaac tgtgcagtcg gtttggcttt ttctgacgaa caaataagat tcgtggccga
      781 caggtggggg tccaccatgt gaaggcatct tcagactcca ataatggagc aatgacgtaa
      841 gggcttacga aataagtaag ggtagtttgg gaaatgtcca ctcacccgtc agtctataaa
      901 tacttagccc ctccctcatt gttaagggag caaaatctca gagagatagt cctagagaga
      961 gaaagagagc aagtagccta gaagtagtca aggcggcgaa gtattcaggc acgtggccag
     1021 gaagaagaaa agccaagacg acgaaaacag gtaagagcta agcttcctgc aggatggaag
     1081 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
     1141 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     1201 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
     1261 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     1321 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     1381 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     1441 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
     1501 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     1561 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
     1621 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
     1681 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
     1741 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     1801 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     1861 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
     1921 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
     1981 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     2041 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     2101 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     2161 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     2221 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     2281 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     2341 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     2401 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     2461 acatcttcga cgctggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     2521 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     2581 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     2641 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     2701 agaagggcgg aaagatcgcc gtgtgacgtc gacgatatga agatgaagat gaaatatttg
     2761 gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt
     2821 atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac
     2881 aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac
     2941 tgtatgtgct atggtatgga ctatggaata tgattaaaga taagccagag ctctggtgac
     3001 ggacgtgggc aggtgtgatg aaaagcctta gggtcaggtg gcacttttcg gggaaatgtg
     3061 cgatgaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctaatgaga
     3121 caataaccct gataaatgct tcaattatat tgaaaaagga agagtatgag tatccaacat
     3181 ttccgtgtcg cccttattcc cttttttgcg gccttctgcc ttcctgtttt tgctcaccca
     3241 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcccgagt gggttacatt
     3301 gaactggatc tcaacagcgg caaaatctta gagagttttc gccccgaaga acgcttccca
     3361 atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt tatcccgtat tgatgctggg
     3421 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtattcacca
     3481 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgtag tgctgccata
     3541 accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg accgaaggag
     3601 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3661 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3721 acaactctcc gcaaactatt aactggcgaa ctacttactc ttgcttcacg ccagcaactc
     3781 attgactgga tggaggcgga caaagttgca ggaccacttc tgcgctcggc acttccggct
     3841 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3901 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3961 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4021 tggtaactgt cagacgaagg gaaataaata gtagcccgcc tgatgtgcgg gctttttttt
     4081 ggtggtggtt accagtgtcg cttagctacc gtaggtcctc ttaggcggtc cgattagcga
     4141 catagtcatc aatgccttga cggcaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4321 ctactaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc g
//
