LOCUS       sequence-254387-       10559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a 
            visual marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pTol2-CreLite
FEATURES             Location/Qualifiers
     source          1..10559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..12,13..200)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    272..292
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3775..3799
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3809..3829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6659..6715
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6716..7414
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             7424..7480
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7484..7504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(8078..8102)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8131..8252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8142..8161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8196..8215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8378..8540,8541..8552)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8569..8586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8740..9328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8820..8839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9499..10290,10291..10359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10122..10141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10360..10464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10532..10550
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
       61 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      121 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      181 ttattttttg gagatcactt cattctattt tcccttgcta ttaccaaacc aattgaattg
      241 cgctgatgcc cagtttaatt taaatagatc tcaactttgt atagaaaagt tgaccagcaa
      301 agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat tctgagtaag
      361 ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata aatgataaca
      421 aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc ctcaaacagt
      481 gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa ttggttcaga
      541 agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc tgattccaca
      601 atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac agcactcact
      661 agctgcccta tatattttat tatattttac atatattatt ttatttattt agctctgagt
      721 gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta cctcaggctc
      781 agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct tgttttcaac
      841 tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca tgcgactttt
      901 ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat ataattatat
      961 tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag atagtcagta
     1021 taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc tatatataag
     1081 tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa tgtagaatga
     1141 cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt gaaaacatcc
     1201 tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa catatacaaa
     1261 aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct ggcagttctg
     1321 tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca gattccacaa
     1381 gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac tgcttgtaga
     1441 ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat atgataatta
     1501 taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac catagcaatc
     1561 attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat ctggtcatgt
     1621 gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact ctcgaccatc
     1681 ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt ttcaacgcaa
     1741 atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta aaaccatagc
     1801 gtattacttt aattatagta aactttcgct ttctttatta caagagacgt tttgtgttga
     1861 ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac cagtaacttg
     1921 cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac gtcaaatagg
     1981 ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta tttttaattt
     2041 atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa agaacccgga
     2101 tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat tttaaaaata
     2161 attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa atgaggaaat
     2221 atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa ctggagaaaa
     2281 ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta gatgttttat
     2341 gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat aacagtaacg
     2401 ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg taagctacga
     2461 gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt aaataaaggc
     2521 tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag aaaaaatata
     2581 aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc tttttgttcc
     2641 tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc caaatgtttc
     2701 cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat aataattttg
     2761 gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca tgcttcagga
     2821 agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca ttacaaacta
     2881 ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc aaatcaaaat
     2941 ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag aaattgtttt
     3001 tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta aaattccaaa
     3061 atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa cagtcttatc
     3121 taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt ttaaatgact
     3181 gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc ttactggccc
     3241 caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca ttattattct
     3301 ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag atgtgcagtt
     3361 tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat gatgcacaaa
     3421 gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga ccctaaattt
     3481 tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata gtccactgtc
     3541 agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag ttttctatta
     3601 ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc attttcgggt
     3661 gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta gaacattgta
     3721 gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt tacagcaagt
     3781 ttgtacaaaa aagcaggctg ccaccatgcc caagaagaag aggaaggtgg tttccggagt
     3841 cgggggtagt ggcggtggcc gtggcggtgg ccgtggcgga gaagaagaac cgtcgtcaag
     3901 tcacactcct aataaccgaa gaggaggaga acaagctcaa tcgtcgggaa cgaaatctct
     3961 cagaccaaga agcaacactg aatcaatgag caaagcaatt caacagtaca ccgtcgacgc
     4021 aagactccac gccgttttcg aacaatccgg cgaatcaggg aaatcattcg actactcaca
     4081 atcactcaaa acgacgacgt acggttcctc tgtacctgag caacagatca cagcttatct
     4141 ctctcgaatc cagcgaggtg gttacattca gcctttcgga tgtatgatcg ccgtcgatga
     4201 atccagtttc cggatcatcg gttacagtga aaacgccaga gaaatgttag ggattatgcc
     4261 tcaatctgtt cctactcttg agaaacctga gattctagct atgggaactg atgtgagatc
     4321 tttgttcact tcttcgagct cgattctact cgagcgtgct ttcgttgctc gagagattac
     4381 cttgttaaat ccggtttgga tccattccaa gaatactggt aaaccgtttt acgccattct
     4441 tcataggatt gatgttggtg ttgttattga tttagagcca gctagaactg aagatcctgc
     4501 gctttctatt gctggtgctg ttcaatcgca gaaactcgcg gttcgtgcga tttctcagtt
     4561 acaggctctt cctggtggag atattaagct tttgtgtgac actgtcgtgg aaagtgtgag
     4621 ggacttgact ggttatgatc gtgttatggt ttataagttt catgaagatg agcatggaga
     4681 agttgtagct gagagtaaac gagatgattt agagccttat attggactgc attatcctgc
     4741 tactgatatt cctcaagcgt caaggttctt gtttaagcag aaccgtgtcc gaatgatagt
     4801 agattgcaat gccacacctg ttcttgtggt ccaggacgat aggctaactc agtctatgtg
     4861 cttggttggt tctactctta gggctcctca tggttgtcac tctcagtata tggctaacat
     4921 gggatctatt gcgtctttag caatggcggt tataatcaat ggaaatgaag atgatgggag
     4981 caatgtagct agtggaagaa gctcgatgag gctttggggt ttggttgttt gccatcacac
     5041 ttcttctcgc tgcataccgt ttccgctaag gtatgcttgt gagtttttga tgcaggcttt
     5101 cggtttacag ttaaacatgg aattgcagtt agctttgcaa atgtcagaga aacgcgtttt
     5161 gagaacgcag acactgttat gtgatatgct tctgcgtgac tcgcctgctg gaattgttac
     5221 acagagtccc agtatcatgg acttagtgaa atgtgacggt gcagcatttc tttaccacgg
     5281 gaagtattac ccgttgggtg ttgctcctag tgaagttcag ataaaagatg ttgtggagtg
     5341 gttgcttgcg aatcatgcgg attcaaccgg attaagcact gatagtttag gcgatgcggg
     5401 gtatcccggt gcagctgcgt taggggatgc tgtgtgcggt atggcagttg catatatcac
     5461 aaaaagagac tttctttttt ggtttcgatc tcacactgcg aaagaaatca aatggggagg
     5521 cgctaagcat catccggagg ataaagatga tgggcaacga atgcatcctc gttcgtcctt
     5581 tcaggctttt cttgaagttg ttaagagccg gagtcagcca tgggaaactg cggaaatgga
     5641 tgcgattcac tcgctccagc ttattctgag agactctttt aaagaatctg gtggttctgc
     5701 cggtggctcc ggttctggct ccagcggtgg cagctctggt gcgtccggca cgggtactgc
     5761 gggtggcact ggcagcggtt ccggtactgg ctctggcaac cggaaatggt ttcccgcaga
     5821 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
     5881 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     5941 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
     6001 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     6061 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     6121 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     6181 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     6241 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     6301 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     6361 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     6421 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     6481 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     6541 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     6601 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatg gaagcggagc
     6661 cacgaacttc tctctgttaa agcaagcagg agatgttgaa gaaaaccccg ggcctatgag
     6721 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
     6781 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     6841 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
     6901 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
     6961 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     7021 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     7081 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     7141 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
     7201 gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata gatccaagaa
     7261 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
     7321 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
     7381 cgacctccct agcaaactgg ggcacaagct taatggaagc ggagccacga acttctctct
     7441 gttaaagcaa gcaggagatg ttgaagaaaa ccccgggcct atgcccaaga agaagaggaa
     7501 ggtgatgttc ttaccaaccg attattgttg caggttaagc gatcaagagt atatggagct
     7561 tgtgtttgag aatggccaga ttcttgcaaa gggccaaaga tccaacgttt ctctgcataa
     7621 tcaacgtacc aaatcgatca tggatttgta tgaggcagag tataacgagg atttcatgaa
     7681 gagtatcatc catggtggtg gtggtgccat cacaaatctc ggggacacgc aggttgttcc
     7741 acaaagtcat gttgctgctg cccatgaaac aaacatgttg gaaagcaata aacatgttga
     7801 cggtggttct gccggtggct ccggttctgg ctccagcggt ggcagctctg gtgcgtccgg
     7861 cacgggtact gcgggtggca ctggcagcgg ttccggtact ggctctggcc tgactgtgca
     7921 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
     7981 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     8041 gtgcagatcc tgggctgcct ggtgcaagct gaactgaacc cagctttctt gtacaaagtg
     8101 gtgatggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     8161 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     8221 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8281 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     8341 gtagatatca agcttaaaca agaatctcta gttttctttc ttgcttttac ttttacttcc
     8401 ttaatactca agtacaattt taatggagta cttttttact tttactcaag taagattcta
     8461 gccagatact tttactttta attgagtaaa attttcccta agtacttgta ctttcacttg
     8521 agtaaaattt ttgagtactt tttacacctc tgggcgctct tccgcttcct cgctcactga
     8581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9181 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9601 gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct caccggctcc
     9661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10501 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
