LOCUS       sequence-25446-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC114
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    85..132
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ctcctttact cattttttct accggtactt tgcgtttctt tttaggtgcc gtcatttttt
       61 ctgagctcgg tacccgggga tcttgaagtt cctattccga agttcctatt ctctagaaag
      121 tataggaact tcagagcgct tttgaagcta gaactagtgg atccacgaga gcgtcggata
      181 ttttacctgg aaataaatta ttttatgatg aaagttaaat acaataagat acaacgggaa
      241 acgtaagaaa aattcaataa aatcaattgt acacgtaggt tttcaagcaa atttaagaat
      301 acaattttac aaagtcctaa atgtcatttt tcctgctcaa acttttgact gaaatgcata
      361 aaaattcgct gataaaaaca agcagaatac gccaaaatag ttgcaaatta taaaatttac
      421 agaattaata caaaaaattc gatattttac tcaaaaaaat ggaaaaaaag aagaaaaata
      481 aattaaactt tcgttgagct ggcctaaaat ctaatagcaa gcgcgctcca ttgccaaacg
      541 gtgtaaacgc ggagcctcgt ttgcgtgtag tttttatcgt aaatcgacac aacttctttc
      601 aaaataaatt atttcttatg agtaa
//
