LOCUS       sequence-25447-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC200
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..48)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(70..90)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(154..174)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    213..260
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    299..323
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttctccttta ctcatttttt ctaccggtac cttacgcttc ttctttggag cagtcatttt
       61 ttctgccggt accttacgct tcttctttgg agcagtcatt ttttctgccg gtaccttacg
      121 cttcttcttt ggagcagtca ttttttctgc cggtacctta cgcttcttct ttggagcagt
      181 cattttttct gagctcggta cccggggatc ttgaagttcc tattccgaag ttcctattct
      241 ctagaaagta taggaacttc agagcgcttt tgaagctaga actagtggat ctgatatcac
      301 cactttgtac aagaaagctg ggtcaccaaa agtagctgaa atatagaaaa actttagatt
      361 ttataacaaa agttatttaa aagttttaaa aggggagaaa aagaaaaaaa tgatgagaaa
      421 aaaagcttgg acaacttgcg cgtagaattt ggcgcctagt ccgcagtcta agtcatcggt
      481 gtttgcagac gtaggattta cgagacaaaa aaaacatgtt tttaatcgaa gttttcgatt
      541 taataagaaa tatcaaattt tcactagaat tttatatttt tgatcggttt acattatttt
      601 tttgtacaca catgaggcgt agc
//
