LOCUS       sequence-25448-e         562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC185
FEATURES             Location/Qualifiers
     source          1..562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(69..89)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(153..173)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    212..259
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    298..322
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctcctttac tcattttttc taccggtacc ttacgcttct tctttggagc agtcattttt
       61 tctgccggta ccttacgctt cttctttgga gcagtcattt tttctgccgg taccttacgc
      121 ttcttctttg gagcagtcat tttttctgcc ggtaccttac gcttcttctt tggagcagtc
      181 attttttctg agctcggtac ccggggatct tgaagttcct attccgaagt tcctattctc
      241 tagaaagtat aggaacttca gagcgctttt gaagctagaa ctagtggatc tgatatcacc
      301 actttgtaca agaaagctgg gtcacgagag cgtcggatat tttacctgga aataaattat
      361 tttatgatga aagttaaata caataagata caacgggaaa cgtaagaaaa attcaataaa
      421 atcaattgta cacgtaggtt ttcaagcaaa tttaagaata caattttaca aagtcctaaa
      481 tgtcattttt cctgctcaaa cttttgactg aaatgcataa aaattcgctg ataaaaacaa
      541 gcagaatacg ccaaaatagt tg
//
