LOCUS       sequence-254532-        4083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C' with Pnos::WUS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_C'5014
FEATURES             Location/Qualifiers
     source          1..4083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..82
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     316..333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        455..634
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        487..670
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2485..2589
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3657..4083,1..162)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
       61 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc acctgcaacg
      361 ccgggcgcgc cgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga
      421 cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa ggagccactc
      481 agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca ttattgcgcg
      541 ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa aatgctccac
      601 tgacgttcca taaattcccc tcggtatcca attagagtct catattcact ctcaatccaa
      661 ataatctgca ccgtacctgc agggtccgag ctaggtcaca gaagcgctca ggaaggccgc
      721 tgagatagag gcatggcggc caatgcgggc ggcggtggag cgggaggagg cagcggcagc
      781 ggcagcgtgg ctgcgccggc ggtgtgccgc cccagcggct cgcggtggac gccgacgccg
      841 gagcagatca ggatgctgaa ggagctgtac tacggctgcg gcatccggtc gcccagctcg
      901 gagcagatcc agcgcatcac cgccatgctg cggcagcacg gcaagatcga gggcaagaac
      961 gtcttctact ggttccagaa ccacaaggcc cgcgagcgcc agaagcgccg cctcaccagc
     1021 ctcgacgtga acgtgcccgc cgccggcgcg gccgacgcca ccaccagcca actcggcgtc
     1081 ctctcgctgt cgtcgccgcc gccttcaggc gcggcgcctc cctcgcccac cctcggcttc
     1141 tacgccgccg gcaatggcgg cggatcggct gtgctgctgg acacgagttc cgactggggc
     1201 agcagcggcg ctgcgatggc caccgagaca tgcttcctcc aggactacat gggcgtgacg
     1261 gacacgggca gctcgtcgca gtggccacgc ttctcgtcgt cggacacgat aatggcggcg
     1321 gccgcggcgc gggcggcgac gacgcgggcg cccgagactc tccctctctt cccgacctgc
     1381 ggcgacgacg gcggcagcgg tagcagcagc tacttgccgt tctggggtgc cgcgtccaca
     1441 actgccggcg ccacttcttc cgttgcgatc cagcagcaac accagctgca ggagcagtac
     1501 agcttttaca gcaacagcaa cagcacccag ctggccggca ccggcaacca agacgtatcg
     1561 gcaacagcag cagcagccgc cgccctggag ctgagcctca gctcatggtg ctccccttac
     1621 cctgctgcag ggagtatgtg agagcaacgc gagctgccac tgctcttcac ttatgtctct
     1681 ggaatggaag gaggaggaag tgagcatagc gttggtgcgt tgctgtcatt gtcctaggtt
     1741 agtagctagt gccagttact agtaagcatc aggcatagga gtatgtagta gaagcatgca
     1801 cgttgccggc cagccaggct ttagacggga aaagaatttg gtgcagccgg ctgcaaaaca
     1861 ggatgtttac agcccccccc tcgagcccta gacttgtcca tcttctggat tggccaagtt
     1921 aattaatgta tgaaataaaa ggatgcacac atagtgacat gctaatcact ataatgtggg
     1981 catcaaagtt gtgtgttatg tgtaattact aattatctga ataagagaaa gagatcatcc
     2041 atatttctta tcctaaatga atgtcacgtg tctttataat tctttgatga accagatgca
     2101 ttttattaac caattccata tacatataaa tattaatcat atataattaa tatcaattgg
     2161 gttagcaaaa caaatctagt ctaggtgtgt tttgctaatt attgggggat agtgcaaaaa
     2221 gaaatctacg ttctcaataa ttcagataga aaacttaata aagtgagata atttacatag
     2281 attgctttta tcctttgata tatgtgaaac catgcatgat ataaggaaaa tagatagaga
     2341 aataattttt tacatcgttg aatatgtaaa caatttaatt caagaagcta ggaatataaa
     2401 tattgaggag tttatgatta gagctctccc actagcaggt gatgaaaagc cttagggtca
     2461 ggtggcactt ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat
     2521 tcaaatatgt atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa
     2581 aggaagagta tgagtatcca acatttccgt gtcgccctta ttcccttttt tgcggccttc
     2641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2701 ttgggtgccc gagtgggtta cattgaactg gatctcaaca gcggcaaaat cttagagagt
     2761 tttcgccccg aagaacgctt cccaatgatg agcactttca aagttctgct atgtggcgcg
     2821 gtgttatccc gtattgatgc tgggcaagag caactcggtc gccgcataca ctattctcag
     2881 aatgacttgg ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2941 agagaattat gtagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3001 acaacgattg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3121 accacgatgc ctgtagcaat ggcaacaact ctccgcaaac tattaactgg cgaactactt
     3181 actcttgctt cacgccagca actcattgac tggatggagg cggacaaagt tgcaggacca
     3241 cttctgcgct cggcacttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3421 ataggtgcct cactgattaa gcattggtaa ctgtcagacg aagggaaata aatagtagcc
     3481 cgcctgatgt gcgggctttt ttttggtggt ggttaccagt gtcgcttagc taccgtaggt
     3541 cctcttaggc ggtccgatta gcgacatagt catcaatgcc ttgacggcaa aatcccttaa
     3601 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3661 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3721 gtggtttgtt tgccggatca agagctacta actctttttc cgaaggtaac tggcttcagc
     3781 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3961 cagcggtcgg gctgaacggg gggttcgtgc tcacagccca gcttggagcg aacgacctac
     4021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4081 aag
//
