LOCUS       sequence-25456-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC238
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..50)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(72..92)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(114..134)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(156..176)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    252..299
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    309..433
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(576..595)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tnttctcctt tactcntttt ttctaccggt accttacgct tcttctttgg agcagtcatt
       61 ttttctgccg gtaccttacg cttcttcttt ggagcagtca ttttttctgc cggtacctta
      121 cgcttcttct ttggagcagt cattttttct gccggtacct tacgcttctt ctttggagca
      181 gtcatttttt ctgagctcct gaaaatgttc tatgttatgt tagtatcatt cgaaacatac
      241 ccggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
      301 gagcatcaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca
      361 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      421 tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
      481 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
      541 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
      601 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
      661 cnnnnnnctg gccngggggg nncnnncatc cnnncncccg ggncgtg
//
