LOCUS       sequence-25457-e         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC160
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..44)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(66..86)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(150..170)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    209..256
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cctttactca ttttttctac cggtacctta cgcttcttct ttggagcagt cattttttct
       61 gccggtacct tacgcttctt ctttggagca gtcatttttt ctgccggtac cttacgcttc
      121 ttctttggag cagtcatttt ttctgccggt accttacgct tcttctttgg agcagtcatt
      181 ttttctgagc tcggtacccg gggatcttga agttcctatt ccgaagttcc tattctctag
      241 aaagtatagg aacttcagag cgccaaaaaa aaaaggcctt ttgggcccaa gcgaggacaa
      301 ttctcatcgt tcgcctaatt tctttgctta ttttttacta gttttccttc ctcctctata
      361 tatttttatg ttttctttta aaccttcctc catatttctg aattgaatgt tgttgcatat
      421 tgtgtgtcct tatttcattt ccatcatcct tttgtgtgtt tcccccttcc accaattccc
      481 cccacgtcct aatgaaatga aagcgagaag ataaataaac ttgcacattg tgttcagatg
      541 agaggagcgg aatacgaatt gggagacgga aagagaaaaa aaatg
//
