LOCUS       sequence-254676-       10727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for hAID-XTEN 
            linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung 
            JK
  TITLE     CRISPR DNA base editors with reduced RNA off-target and self-editing
            activities.
  JOURNAL   Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6.
  PUBMED    31477922
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6."
COMMENT     Sequence Label: hAID-BE3 (pRZ352)
FEATURES             Location/Qualifiers
     source          1..10727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(493..1284,1285..1353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1116..1135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1354..1458)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1489..1868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1870..2147
                     /label=chicken beta-actin promoter
     intron          2148..3164
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3088..3110
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3172..3191
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3257..3266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3263..3856
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             join(3905..7303,7304..7306,7307..7903,7904,7905..7907,
                     7908..7909,7910..7912,7913..8005)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3905..3928,3929..3931,3932..8005)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8018..8266
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8279..8299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8318..8374
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8375..8377,8378..8380,8381..9094)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8375..8377,8378..8380,8381..9091)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8420..8441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8681..8700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9028..9049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9181..9200)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9246..9301
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9300..9319)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9662..9678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9662..9678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9675..9697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9686..9702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9710..9716,9717..9734,9735..9740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9755..9776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9835..10030
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9881..10016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10036..10170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10073..10092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10127..10146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10238..10255)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10489..10508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cskkyagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     1501 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1561 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1621 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1681 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1741 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1801 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1861 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1921 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1981 gggggggggg gcgcgcgccg gggggggsgg gggggggsgg ggggsggggs ggggcgaggc
     2041 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     2101 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     2161 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     2221 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     2281 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     2341 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     2401 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     2461 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2521 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2581 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2641 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2701 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2761 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2821 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2881 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2941 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     3001 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     3061 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     3121 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     3181 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     3241 ccgggatcca ccggtcgcca ccatggatag cctcttgatg aatagacgca agttcctgta
     3301 tcagtttaaa aacgtgagat gggcaaaagg ccgacgagag acatatctgt gctatgtcgt
     3361 taagcgcaga gattcagcca ccagtttctc tctcgacttc ggctacctgc ggaacaagaa
     3421 tggttgccat gttgagctcc tgttcctgag gtatatcagc gactgggatt tggacccagg
     3481 gcggtgctat agggtgacat ggtttacctc ctggtcacct tgttatgact gcgcgcggca
     3541 tgttgccgat tttctgagag ggaaccctaa cctgtctctg aggatcttca ccgcgcgact
     3601 gtacttctgt gaggaccgga aagccgaacc cgagggactg agacgcctcc acagagcggg
     3661 tgtgcagatt gccataatga cctttaagga ctacttctac tgctggaaca ccttcgtcga
     3721 aaatcacgag cggactttca aggcttggga aggattgcat gaaaacagcg tcaggctttc
     3781 caggcagctt cgccgcattc ttctcccgtt gtacgaggtt gatgacctca gagatgcctt
     3841 tagaacactg ggactgagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga
     3901 aagtgataaa aagtattcta ttggtttagc catcggcact aattccgttg gatgggctgt
     3961 cataaccgat gaatacaaag taccttcaaa gaaatttaag gtgttgggga acacagaccg
     4021 tcattcgatt aaaaagaatc ttatcggtgc cctcctattc gatagtggcg aaacggcaga
     4081 ggcgactcgc ctgaaacgaa ccgctcggag aaggtataca cgtcgcaaga accgaatatg
     4141 ttacttacaa gaaattttta gcaatgagat ggccaaagtt gacgattctt tctttcaccg
     4201 tttggaagag tccttccttg tcgaagagga caagaaacat gaacggcacc ccatctttgg
     4261 aaacatagta gatgaggtgg catatcatga aaagtaccca acgatttatc acctcagaaa
     4321 aaagctagtt gactcaactg ataaagcgga cctgaggtta atctacttgg ctcttgccca
     4381 tatgataaag ttccgtgggc actttctcat tgagggtgat ctaaatccgg acaactcgga
     4441 tgtcgacaaa ctgttcatcc agttagtaca aacctataat cagttgtttg aagagaaccc
     4501 tataaatgca agtggcgtgg atgcgaaggc tattcttagc gcccgcctct ctaaatcccg
     4561 acggctagaa aacctgatcg cacaattacc cggagagaag aaaaatgggt tgttcggtaa
     4621 ccttatagcg ctctcactag gcctgacacc aaattttaag tcgaacttcg acttagctga
     4681 agatgccaaa ttgcagctta gtaaggacac gtacgatgac gatctcgaca atctactggc
     4741 acaaattgga gatcagtatg cggacttatt tttggctgcc aaaaacctta gcgatgcaat
     4801 cctcctatct gacatactga gagttaatac tgagattacc aaggcgccgt tatccgcttc
     4861 aatgatcaaa aggtacgatg aacatcacca agacttgaca cttctcaagg ccctagtccg
     4921 tcagcaactg cctgagaaat ataaggaaat attctttgat cagtcgaaaa acgggtacgc
     4981 aggttatatt gacggcggag cgagtcaaga ggaattctac aagtttatca aacccatatt
     5041 agagaagatg gatgggacgg aagagttgct tgtaaaactc aatcgcgaag atctactgcg
     5101 aaagcagcgg actttcgaca acggtagcat tccacatcaa atccacttag gcgaattgca
     5161 tgctatactt agaaggcagg aggattttta tccgttcctc aaagacaatc gtgaaaagat
     5221 tgagaaaatc ctaacctttc gcatacctta ctatgtggga cccctggccc gagggaactc
     5281 tcggttcgca tggatgacaa gaaagtccga agaaacgatt actccatgga attttgagga
     5341 agttgtcgat aaaggtgcgt cagctcaatc gttcatcgag aggatgacca actttgacaa
     5401 gaatttaccg aacgaaaaag tattgcctaa gcacagttta ctttacgagt atttcacagt
     5461 gtacaatgaa ctcacgaaag ttaagtatgt cactgagggc atgcgtaaac ccgcctttct
     5521 aagcggagaa cagaagaaag caatagtaga tctgttattc aagaccaacc gcaaagtgac
     5581 agttaagcaa ttgaaagagg actactttaa gaaaattgaa tgcttcgatt ctgtcgagat
     5641 ctccggggta gaagatcgat ttaatgcgtc acttggtacg tatcatgacc tcctaaagat
     5701 aattaaagat aaggacttcc tggataacga agagaatgaa gatatcttag aagatatagt
     5761 gttgactctt accctctttg aagatcggga aatgattgag gaaagactaa aaacatacgc
     5821 tcacctgttc gacgataagg ttatgaaaca gttaaagagg cgtcgctata cgggctgggg
     5881 acgattgtcg cggaaactta tcaacgggat aagagacaag caaagtggta aaactattct
     5941 cgattttcta aagagcgacg gcttcgccaa taggaacttt atgcagctga tccatgatga
     6001 ctctttaacc ttcaaagagg atatacaaaa ggcacaggtt tccggacaag gggactcatt
     6061 gcacgaacat attgcgaatc ttgctggttc gccagccatc aaaaagggca tactccagac
     6121 agtcaaagta gtggatgagc tagttaaggt catgggacgt cacaaaccgg aaaacattgt
     6181 aatcgagatg gcacgcgaaa atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg
     6241 gatgaagaga atagaagagg gtattaaaga actgggcagc cagatcttaa aggagcatcc
     6301 tgtggaaaat acccaattgc agaacgagaa actttacctc tattacctac aaaatggaag
     6361 ggacatgtat gttgatcagg aactggacat aaaccgttta tctgattacg acgtcgatca
     6421 cattgtaccc caatcctttt tgaaggacga ttcaatcgac aataaagtgc ttacacgctc
     6481 ggataagaac cgagggaaaa gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa
     6541 gaactattgg cggcagctcc taaatgcgaa actgataacg caaagaaagt tcgataactt
     6601 aactaaagct gagaggggtg gcttgtctga acttgacaag gccggattta ttaaacgtca
     6661 gctcgtggaa acccgccaaa tcacaaagca tgttgcacag atactagatt cccgaatgaa
     6721 tacgaaatac gacgagaacg ataagctgat tcgggaagtc aaagtaatca ctttaaagtc
     6781 aaaattggtg tcggacttca gaaaggattt tcaattctat aaagttaggg agataaataa
     6841 ctaccaccat gcgcacgacg cttatcttaa tgccgtcgta gggaccgcac tcattaagaa
     6901 atacccgaag ctagaaagtg agtttgtgta tggtgattac aaagtttatg acgtccgtaa
     6961 gatgatcgcg aaaagcgaac aggagatagg caaggctaca gccaaatact tcttttattc
     7021 taacattatg aatttcttta agacggaaat cactctggca aacggagaga tacgcaaacg
     7081 acctttaatt gaaaccaatg gggagacagg tgaaatcgta tgggataagg gccgggactt
     7141 cgcgacggtg agaaaagttt tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt
     7201 gcagaccgga gggttttcaa aggaatcgat tcttccaaaa aggaatagtg ataagctcat
     7261 cgctcgtaaa aaggactggg acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc
     7321 ctattctgtc ctagtagtgg caaaagttga gaagggaaaa tccaagaaac tgaagtcagt
     7381 caaagaatta ttggggataa cgattatgga gcgctcgtct tttgaaaaga accccatcga
     7441 cttccttgag gcgaaaggtt acaaggaagt aaaaaaggat ctcataatta aactaccaaa
     7501 gtatagtctg tttgagttag aaaatggccg aaaacggatg ttggctagcg ccggagagct
     7561 tcaaaagggg aacgaactcg cactaccgtc taaatacgtg aatttcctgt atttagcgtc
     7621 ccattacgag aagttgaaag gttcacctga agataacgaa cagaagcaac tttttgttga
     7681 gcagcacaaa cattatctcg acgaaatcat agagcaaatt tcggaattca gtaagagagt
     7741 catcctagct gatgccaatc tggacaaagt attaagcgca tacaacaagc acagggataa
     7801 acccatacgt gagcaggcgg aaaatattat ccatttgttt actcttacca acctcggcgc
     7861 tccagccgca ttcaagtatt ttgacacaac gatagatcgc aaacgataca cttctaccaa
     7921 ggaggtgcta gacgcgacac tgattcacca atccatcacg ggattatatg aaactcggat
     7981 agatttgtca cagcttgggg gtgactctgg tggttctact aatctgtcag atattattga
     8041 aaaggagacc ggtaagcaac tggttatcca ggaatccatc ctcatgctcc cagaggaggt
     8101 ggaagaagtc attgggaaca agccggaaag cgatatactc gtgcacaccg cctacgacga
     8161 gagcaccgac gagaatgtca tgcttctgac tagcgacgcc cctgaataca agccttgggc
     8221 tctggtcata caggatagca acggtgagaa caagattaag atgctctctg gtggttctcc
     8281 caagaagaag aggaaagtcg gaggcggtgg aagcggcgca acaaacttct ctctgctgaa
     8341 acaagccgga gatgtcgaag agaatcctgg accgatggtg agcaagggcg aggagctgtt
     8401 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     8461 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     8521 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     8581 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     8641 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     8701 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     8761 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     8821 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     8881 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     8941 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     9001 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     9061 gatcactctc ggcatggacg agctgtacaa gtgaagcggc cgcactcctc aggtgcaggc
     9121 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     9181 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     9241 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     9301 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     9361 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     9421 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     9481 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     9541 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     9601 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     9661 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9721 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9781 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     9841 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9961 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10021 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10141 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
    10201 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10261 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10321 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10381 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10441 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10501 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10561 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10621 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10681 agcccgaccg ctgcgcctta tccgstrrsy wkygtsywsr rwccmmc
//
