LOCUS       sequence-254743-       17262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under 
            the 35s promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 17262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pGWB21_TaNAM-A1
FEATURES             Location/Qualifiers
     source          1..17262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5842..5871
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5878..5907
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5923..5952
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5959..5988
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5995..6024
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6040..6069
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6076..6105
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6112..6141
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6157..6186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6193..6222
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6241..6265
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7520..7544)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7570..7822
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8251..8503)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10006..10350)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10492..10509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10492..10508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10501..10523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10648..10667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10858..10879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11126..11150
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12746..13894)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14193..14987)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15179..15946
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(16639..17262,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 atgagcgggt taattaacgg tgaacaaaag ctaatctccg aggaagactt gaacggtgaa
     5881 caaaaattaa tctcagaaga agacttgaac ggactcgacg gtgaacaaaa gttgatttct
     5941 gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa cggtgaacaa
     6001 aaattaatct cagaagaaga cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa
     6061 gaagatttga acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa
     6121 ttaatctcag aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa
     6181 gatttgaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtag cgctgttatc
     6241 acaagtttgt acaaaaaagc aggctccgaa ttcgccctta tgaggtccat gggcagctcc
     6301 gactcatcct ccggctcggc gcaaaaagca gcgcggcatc agcatgagcc gccgcctccg
     6361 cggcagcggg gctcggcgcc ggagctccca ccgggcttcc ggttccaccc gacggacgag
     6421 gagctggtcg tgcactacct caagaagaag gccgccaagg tgccgctccc cgtcaccatc
     6481 atcgccgagg tggatctcta caagttcgac ccatgggagc tccccgagaa ggcgaccttc
     6541 ggggagcagg agtggtactt cttcagcccg cgcgaccgca agtaccccaa cggcgcgcgg
     6601 ccgaaccggg cggcgacgtc gggctactgg aaggccaccg gcacggacaa acctatcctg
     6661 gcctcgggga cggggtgcgg cctggtccgg gagaagctcg gcgtcaagaa ggcgctcgtc
     6721 ttctaccgcg ggaagccgcc caagggcctc aaaaccaact ggatcatgca cgagtaccgc
     6781 ctcaccgacg cgtctggctc caccaccacc agccggccgc cgccgcctgt gaccggcggg
     6841 agccgggctg cagcctctct gaggttggac gactgggtgc tgtgccgcat ctacaagaag
     6901 atcaacaagg ccgcggccgg agatcagcag aggagcacgg agtgcgagga ctccgtggag
     6961 gacgcggtca ccgcgtaccc gctctatgcc acggcgggca tggccggtgc aggtgcgcat
     7021 ggcagcaact acgcttcacc ttcactgctc catcatcagg acagccattt cctggagggc
     7081 ctgttcacag cagacgacgc cggcctctcg gcgggcgcca cctcgctgag ccacctggcc
     7141 gcggcggcga gggcgagccc ggctccgacc aaacagtttc tcgccccgtc gtcttcaacc
     7201 ccgttcaact ggctcgatgc gtcacccgcc ggcatcctgc cacaggcaag gaatttccct
     7261 gggtttaaca ggagcagaaa cgtcggcaat atgtcgctgt catcgacggc cgacatggct
     7321 ggcgcggccg gcaatgcggt gaacgccatg tccgcattta tgaatcctct ccccgtgcaa
     7381 gacgggacgt accatcaaca ccatgtcatc ctcggcgccc cactggcgcc agaggctacc
     7441 acaggcggcg ccacctctgg tttccagcat cccgtccaag tatccggcgt gaactggaat
     7501 ccctgaaagg gcgaattcga cccagctttc ttgtacaaag tggtgataac ctagagctcg
     7561 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     7621 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     7681 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     7741 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     7801 gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc
     7861 gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg acgtaggcct
     7921 atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc
     7981 gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt tcacaagaca
     8041 gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg acgaattagt
     8101 gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac ttccaattcg
     8161 aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc tcgattgctc
     8221 atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac accgcgcgcg
     8281 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     8341 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     8401 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     8461 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg acgcactcct
     8521 tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc
     8581 gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa tcttgccttt
     8641 ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt tggcaatagc
     8701 tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc gatgttccat
     8761 gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt attccctcaa
     8821 agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat tgagacgcaa
     8881 tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga cgttgcaaac
     8941 gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     9001 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     9061 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     9121 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     9181 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     9241 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     9301 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     9361 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     9421 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     9481 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     9541 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     9601 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     9661 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     9721 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     9781 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     9841 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     9901 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     9961 ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct ctccaaatga
    10021 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
    10081 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    10141 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    10201 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    10261 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    10321 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
    10381 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga tctctaggac
    10441 gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt cactggccgt
    10501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10621 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    10681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    10741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
    10801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    10861 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
    10921 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
    10981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
    11041 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
    11101 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
    11161 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
    11221 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
    11281 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
    11341 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc
    11401 ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg
    11461 tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc
    11521 tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct
    11581 caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc
    11641 tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
    11701 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
    11761 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
    11821 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
    11881 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
    11941 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
    12001 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
    12061 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
    12121 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
    12181 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
    12241 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
    12301 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
    12361 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
    12421 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
    12481 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
    12541 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
    12601 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
    12661 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
    12721 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    12781 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    12841 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    12901 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    12961 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    13021 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    13081 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    13141 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    13201 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    13261 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    13321 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    13381 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    13441 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    13501 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    13561 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    13621 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    13681 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    13741 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    13801 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    13861 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    13921 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    13981 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    14041 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    14101 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    14161 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    14221 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    14281 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    14341 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    14401 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    14461 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    14521 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    14581 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    14641 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    14701 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    14761 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    14821 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    14881 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    14941 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    15001 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    15061 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    15121 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    15181 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
    15241 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
    15301 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
    15361 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
    15421 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
    15481 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
    15541 cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg
    15601 gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg
    15661 cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg
    15721 gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag
    15781 caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg
    15841 gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg
    15901 gcattatctg aacataaaac actatcaata agttggagtc attacccaat tatgatagaa
    15961 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
    16021 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
    16081 cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat
    16141 attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat
    16201 atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa
    16261 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
    16321 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
    16381 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
    16441 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
    16501 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
    16561 tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
    16621 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
    16681 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    16741 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    16801 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    16861 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    16921 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    16981 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    17041 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    17101 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    17161 gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
    17221 catagacggc cgccagccca gcggcgaggg caaccagccc gg
//
