LOCUS       sequence-25476-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC296
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(162..209)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             286..306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             328..348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             370..390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             412..432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(587..605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actattctca ttttaatttt cagagcttaa aaatgggtga aatcactcac aacgatggat
       61 acgctaacaa cttggaaatg aaataagctt gcatgcctgc aggtcgactc tagaccgcgg
      121 ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct atactttcta
      181 gagaatagga acttcggaat aggaacttca agatccccgg ggtatgtttc gaatgatact
      241 aacataacat agaacatttt caggagctca gaaaaaatga ctgctccaaa gaagaagcgt
      301 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa
      361 atgactgctc caaagaagaa gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag
      421 aagcgtaagg taccggtaga cgataaggat ccgatggtgg cctcctccga ggacgtcatc
      481 aaagagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
      541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      601 accaagggcg g
//
