LOCUS       sequence-254819-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression human MSH6 protein using baculovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
  TITLE     In vitro studies of DNA mismatch repair proteins.
  JOURNAL   Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 
            10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.
  PUBMED    21329650
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2011.02.017"; journalName: "Anal Biochem"; date: 
            "2011-06-15- 15"; volume: "413"; issue: "2"; pages: "179-84"
COMMENT     Sequence Label: pFB1_hMSH6
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1014..1019,1020..1036,1037..1042))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1394..1485
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1410..1427
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5760..5894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5797..5816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5851..5870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5923..6088
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6272..6726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6359..6378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6568..6589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6752..6856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6857..6925,6926..7717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7075..7094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7888..8476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8377..8396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8630..8647
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
      841 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
      901 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      961 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     1021 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1081 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1141 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     1201 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     1261 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     1321 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     1381 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     1441 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     1501 taccgtccca ccatcgggcg cggatccatg tcgcgacaga gcaccctgta cagcttcttc
     1561 cccaagtctc cggcgctgag tgatgccaac aaggcctcgg ccagggcctc acgcgaaggc
     1621 ggccgtgccg ccgctgcccc cggggcctct ccttccccag gcggggatgc ggcctggagc
     1681 gaggctgggc ctgggcccag gcccttggcg cgatccgcgt caccgcccaa ggcgaagaac
     1741 ctcaacggag ggctgcggag atcggtagcg cctgctgccc ccaccagttg tgacttctca
     1801 ccgggagatt tggtttgggc caagatggag ggttacccct ggtggccttg tctggtttac
     1861 aaccacccct ttgatggaac attcatccgc gagaaaggga aatcagtccg tgttcatgta
     1921 cagttttttg atgacagccc aacaaggggc tgggttagca aaaggctttt aaagccatat
     1981 acaggttcaa aatcaaagga agcccagaag ggaggtcatt tttacagtgc aaagcctgaa
     2041 atactgagag caatgcaacg tgcagacgaa gccttaaata aagacaagat taagaggctt
     2101 gaattggcag tttgtgatga gccctcagag ccagaagagg aagaagagat ggaggtaggc
     2161 acaacttacg taacagataa gagtgaagaa gataatgaaa ttgagagtga agaggaagta
     2221 cagcctaaga cacaaggatc taggcgaagt agccgccaaa taaaaaaacg aagggtcata
     2281 tcagattctg agagtgacat tggtggctct gatgtggaat ttaagccaga cactaaggag
     2341 gaaggaagca gtgatgaaat aagcagtgga gtgggggata gtgagagtga aggcctgaac
     2401 agccctgtca aagttgctcg aaagcggaag agaatggtga ctggaaatgg ctctcttaaa
     2461 aggaaaagct ctaggaagga aacgccctca gccaccaaac aagcaactag catttcatca
     2521 gaaaccaaga atactttgag agctttctct gcccctcaaa attctgaatc ccaagcccac
     2581 gttagtggag gtggtgatga cagtagtcgc cctactgttt ggtatcatga aactttagaa
     2641 tggcttaagg aggaaaagag aagagatgag cacaggagga ggcctgatca ccccgatttt
     2701 gatgcatcta cactctatgt gcctgaggat ttcctcaatt cttgtactcc tgggatgagg
     2761 aagtggtggc agattaagtc tcagaacttt gatcttgtca tctgttacaa ggtggggaaa
     2821 ttttatgagc tgtaccacat ggatgctctt attggagtca gtgaactggg gctggtattc
     2881 atgaaaggca actgggccca ttctggcttt cctgaaattg catttggccg ttattcagat
     2941 tccctggtgc agaagggcta taaagtagca cgagtggaac agactgagac tccagaaatg
     3001 atggaggcac gatgtagaaa gatggcacat atatccaagt atgatagagt ggtgaggagg
     3061 gagatctgta ggatcattac caagggtaca cagacttaca gtgtgctgga aggtgatccc
     3121 tctgagaact acagtaagta tcttcttagc ctcaaagaaa aagaggaaga ttcttctggc
     3181 catactcgtg catatggtgt gtgctttgtt gatacttcac tgggaaagtt tttcataggt
     3241 cagttttcag atgatcgcca ttgttcgaga tttaggactc tagtggcaca ctatccccca
     3301 gtacaagttt tatttgaaaa aggaaatctc tcaaaggaaa ctaaaacaat tctaaagagt
     3361 tcattgtcct gttctcttca ggaaggtctg atacccggct cccagttttg ggatgcatcc
     3421 aaaactttga gaactctcct tgaggaagaa tattttaggg aaaagctaag tgatggcatt
     3481 ggggtgatgt taccccaggt gcttaaaggt atgacttcag agtctgattc cattgggttg
     3541 acaccaggag agaaaagtga attggccctc tctgctctag gtggttgtgt cttctacctc
     3601 aaaaaatgcc ttattgatca ggagctttta tcaatggcta attttgaaga atatattccc
     3661 ttggattctg acacagtcag cactacaaga tctggtgcta tcttcaccaa agcctatcaa
     3721 cgaatggtgc tagatgcagt gacattaaac aacttggaga tttttctgaa tggaacaaat
     3781 ggttctactg aaggaaccct actagagagg gttgatactt gccatactcc ttttggtaag
     3841 cggctcctaa agcaatggct ttgtgcccca ctctgtaacc attatgctat taatgatcgt
     3901 ctagatgcca tagaagacct catggttgtg cctgacaaaa tctccgaagt tgtagagctt
     3961 ctaaagaagc ttccagatct tgagaggcta ctcagtaaaa ttcataatgt tgggtctccc
     4021 ctgaagagtc agaaccaccc agacagcagg gctataatgt atgaagaaac tacatacagc
     4081 aagaagaaga ttattgattt tctttctgct ctggaaggat tcaaagtaat gtgtaaaatt
     4141 atagggatca tggaagaagt tgctgatggt tttaagtcta aaatccttaa gcaggtcatc
     4201 tctctgcaga caaaaaatcc tgaaggtcgt tttcctgatt tgactgtaga attgaaccga
     4261 tgggatacag cctttgacca tgaaaaggct cgaaagactg gacttattac tcccaaagca
     4321 ggctttgact ctgattatga ccaagctctt gctgacataa gagaaaatga acagagcctc
     4381 ctggaatacc tagagaaaca gcgcaacaga attggctgta ggaccatagt ctattggggg
     4441 attggtagga accgttacca gctggaaatt cctgagaatt tcaccactcg caatttgcca
     4501 gaagaatacg agttgaaatc taccaagaag ggctgtaaac gatactggac caaaactatt
     4561 gaaaagaagt tggctaatct cataaatgct gaagaacgga gggatgtatc attgaaggac
     4621 tgcatgcggc gactgttcta taactttgat aaaaattaca aggactggca gtctgctgta
     4681 gagtgtatcg cagtgttgga tgttttactg tgcctggcta actatagtcg agggggtgat
     4741 ggtcctatgt gtcgcccagt aattctgttg ccggaagata cccccccctt cttagagctt
     4801 aaaggatcac gccatccttg cattacgaag actttttttg gagatgattt tattcctaat
     4861 gacattctaa taggctgtga ggaagaggag caggaaaatg gcaaagccta ttgtgtgctt
     4921 gttactggac caaatatggg gggcaagtct acgcttatga gacaggctgg cttattagct
     4981 gtaatggccc agatgggttg ttacgtccct gctgaagtgt gcaggctcac accaattgat
     5041 agagtgttta ctagacttgg tgcctcagac agaataatgt caggtgaaag tacatttttt
     5101 gttgaattaa gtgaaactgc cagcatactc atgcatgcaa cagcacattc tctggtgctt
     5161 gtggatgaat taggaagagg tactgcaaca tttgatggga cggcaatagc aaatgcagtt
     5221 gttaaagaac ttgctgagac tataaaatgt cgtacattat tttcaactca ctaccattca
     5281 ttagtagaag attattctca aaatgttgct gtgcgcctag gacatatggc atgcatggta
     5341 gaaaatgaat gtgaagaccc cagccaggag actattacgt tcctctataa attcattaag
     5401 ggagcttgtc ctaaaagcta tggctttaat gcagcaaggc ttgctaatct cccagaggaa
     5461 gttattcaaa agggacatag aaaagcaaga gaatttgaga agatgaatca gtcactacga
     5521 ttatttcggg aagtttgcct ggctagtgaa aggtcaactg tagatgctga agctgtccat
     5581 aaattgctga ctttgattaa ggaattatag atctcgaggc atgcggtacc aagcttgtcg
     5641 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     5701 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5761 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5821 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5881 atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc
     5941 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     6001 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     6061 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa
     6121 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca
     6181 cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca
     6241 acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6301 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6361 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6421 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6481 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6541 ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca
     6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6661 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6721 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8521 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8641 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8701 ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     8761 ggatgccctg cgtaagcggg
//
