LOCUS       sequence-25483-e         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC306
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(162..182)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    259..306
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(513..529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(513..529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(526..548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    537..553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(561..567,568..585,586..591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttggtcacct tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc gccctcgccc
       61 tcgatctcga actcgtggcc gttcacggtg ccctccatgc gcaccttgaa gcgcatgaac
      121 tcggtgatga cgttctcgga ggaggccatg gtggcgaccg gtaccttacg cttcttcttt
      181 ggagcagtca ttttttctga gctcctgaaa atgttctatg ttatgttagt atcattcgaa
      241 acataccccg gggatcttga agttcctatt ccgaagttcc tattctctag aaagtatagg
      301 aacttcagag cgcttttgaa gctagaacta gtggatccgc gccaaaaccg cggtctagag
      361 tcgacctgca ggcatgcaag cttatttcat ttccaagttg ttagcgtatc catcgttgtg
      421 agtgatttca gccattttta agctctgaaa atttaaatga gaatagttag ttagtaagat
      481 catgtttaaa cttacagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      541 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcct
//
