LOCUS       sequence-25494-e         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC310
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(180..227)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             304..324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(482..500)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aactgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
       61 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc atgcctgcag
      121 gtcgactcta gaccgcggtt ttggcgcgga tccactagtt ctagcttcaa aagcgctctg
      181 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaag atccccgggg
      241 tatgtttcga atgatactaa cataacatag aacattttca ggagctcaga aaaaatgact
      301 gctccaaaga agaagcgtaa ggtaccggta gaaaaaatgg tgagcaaggg cgaggaggat
      361 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      481 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      541 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactac
//
