LOCUS       sequence-255226-         245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of 3WJ repressor index 19 switch RNA with a 
            GFP-ASV reporter via the Ptet* promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Kim J, Zhou Y, Carlson PD, Teichmann M, Chaudhary S, Simmel FC, 
            Silver PA, Collins JJ, Lucks JB, Yin P, Green AA
  TITLE     De novo-designed translation-repressing riboregulators for 
            multi-input cellular logic.
  JOURNAL   Nat Chem Biol. 2019 Dec;15(12):1173-1182. doi: 
            10.1038/s41589-019-0388-1. Epub 2019 Nov 4.
  PUBMED    31686032
REFERENCE   2  (bases 1 to 245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0388-1"; journalName: "Nat Chem Biol"; date: 
            "2019-12"; volume: "15"; issue: "12"; pages: "1173-1182"
COMMENT     Sequence Label: pYZ_Ptet*_3WJrep_N19_switch
FEATURES             Location/Qualifiers
     source          1..245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..41
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        79..128
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 ttttcagcag gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga
       61 tagagatact gagcacagct gtcaccggat gtgctttccg gtctgatgag tccgtgagga
      121 cgaaacagcc tctacaaata attttgttta aactaatcag atctacttgt tatagttatg
      181 aacagaggag acataacatg aacaagcacc taacaagact aatcaacctg gcggcagcgc
      241 aaaag
//
