LOCUS       sequence-25544-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pcDNA5 HA-Amida
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             143..169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggggnatta gcaganctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcc gccaccatgg cttacccata
      121 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatctctcga
      181 gatggaattg gagcagagag aagggaccat ggcagccgtg ggctttgagg agttctcagc
      241 gccgccaggc tcagagttgg cgttgcctcc cctatttggt ggccacatcc tggagagcga
      301 gctggagacg gaagtggagt ttgtgtcagg tggtctgggc ggctcagggc tccgggagcg
      361 agatgaagag gaagaggcag cccggggtcg gcggcggcgc cagcgggaat taaatcgcag
      421 aaagtaccag gcactaggtc ggcgctgccg ggagatcgag caggtgaacg agcgggtcct
      481 gaacaggctc catcaggtgc agaggataac tcggaggctg cagcaggaac ggaggttcct
      541 catgagagtg ctggactcct acggggatga ctaccgggcc agccagttca ccattgtgct
      601 ggaggatgag ggcagccagg gcacggatgc ccccacccca ggcaatgcgg agaatacang
      661 naaaaaaaaa aaancatttn ccccncccaa aanganntcc tgcnnccccc aganccggca
      721 gcccagcccc cggtganggg cccantgggc cggaannagc ggcnagtgcc acggatgacc
      781 ccaaaacaa
//
