LOCUS       sequence-25545-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pcDNA5 HA-CCDC95
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgtggggnnt tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gctggctagc cgccaccatg gcttacccat
      121 acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca ggatctctcg
      181 agatgaacgg gccggcggac ggcgaagtgg actacaaaaa aaaataccgg aatctgaagc
      241 ggaagctcaa gttcctcatc tacgagcacg agtgcttcca ggaggagctg aggaaagcgc
      301 aaaggaaatt actgaaggtg tcccgggaca agagtttcct cctagaccga cttctgcagt
      361 acgagaacgt ggatgaagac tcttcggact cagatgccac tgcatcatca gataacagcg
      421 agacggaggg gacacccaag ttgtctgaca caccggcccc taagaggaag agaagccctc
      481 cgctgggggg cgccccctct ccctccagcc tctccctgcc tccttcaaca gggtttcccc
      541 ttcaggcctc cggggtcccc tccccatacc tgagctcgct ggcctcctcc cgctaccccc
      601 cattcccttc tgactacctg gccctgcagc tgcccgagcc cagtcccctg aggcccaagc
      661 gggagaaacg gccccgcctg ccccggaaac tcaagatggg cggtgggacc ccccgactgc
      721 cctgtgggag ggccgctgac cttccctggc cggggttctg gggctggggt cgganaaatg
      781 ccntgacccc cctcccaccc cctaagatgc cccc
//
