LOCUS       sequence-25549-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(111..314)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(140..160)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        315..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cnccggtnac gctctcgccc ttgctcccat ggtggcgacc ggtggatccc ggagcttgag
       61 ctcgagatct gagtccggta gcgctagcgg atctgacggt tcactaaacc agctctgctt
      121 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      181 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      241 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      301 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      361 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      421 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      481 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      541 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      601 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      661 gattactatt aataactaat gcatggcggt aatacggtta tccacagaat caggggataa
      721 cgcaggaaag aacatgaaga nnaagnccaa aaaaggccna gaacc
//
