LOCUS       sequence-255551-       12512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-36-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5314..5379
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5389..5977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5442..5462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5860..5871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5991..6490
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6026..6049)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6423..6442
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6511..7110
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6511..6530)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7007..7027
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7248..7481
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7553..7674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7590..7609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7644..7663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7714..7849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7869..7888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7870..7888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7898..7915)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7898..7914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7907..7929)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8056..8511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8143..8162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8353..8374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8537..8641
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8642..8710,8711..9502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8860..8879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9673..10261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10162..10181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10415..10432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10549..10570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10585..10590,10591..10608,10609..10615)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10623..10639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10628..10650
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10647..10663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10647..10663
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10682..10702
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10684..10702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10730..10956
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10957..11137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11184..11309
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11802..12035
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12220..12264
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atggaacctc aggcactata tgatgaagta agaactgtgc caattgcaaa gctggatagg
     2761 acagttgctg agaaagctgt taaaaaatat gtagaagatg aaatggcaag gctccctgat
     2821 agattgtcag taacttggcc tgaaggagat gaattattgc ctaatgaggt taggcctgct
     2881 ggaaccccta ttggtgcgtt aagaattgaa atactgaata aaaaagggga agcaatgcaa
     2941 aagcttccag gaacaagcca tggagggtca aagaaactcc tggttgagct caaagttatt
     3001 ttacattctt caagtggaaa taaagagatt atttcgcata ttagtcaaca tggaggaaaa
     3061 tggccttact ggtttaaaaa aatggaaaat attcagaagt tggggaatta taccttgaaa
     3121 ttacaagttg tgttgaatga aagtaatgca gacacttatg caggaagacc actaccatct
     3181 aaagcaatta agttttctgt taaagagggt aagccagaga aattttcatt tggtcttctg
     3241 gatcttcctt ttcgtgttgg agttccattt aatatccctc tggagtttca ggatgaattt
     3301 ggtcatacca gtcaactagt aactgatatt cagccagttc ttgaagcaag tggtttatct
     3361 ttacattatg aagaaataac caaaggacca aattgtgtaa ttcgaggtgt tacagccaag
     3421 ggccctgtaa actcttgtca aggcaagaat tataatctga aggttactct gcctggctta
     3481 aaagaagact cacagatttt gaaaattaga ttactacctg gtcaccctcg tcgactgaaa
     3541 gtgaaacctg attctgaaat tttagttata gaaaatggaa cagctttccc atttcaggtg
     3601 gaagttttag atgaatcaga caacataaca gcacaaccaa aattgattgt tcattgtaag
     3661 ttttcaggtg ctccaaacct tccagtctat gttgtagatt gcagtagttc tggaaccagt
     3721 attttaacag gatctgcaat tcaagttcag aatattaaaa aagaccagac gcttaaagca
     3781 agaattgaaa tacctagttg taaagatgtg gcacctgtgg agaagactat taagttgctt
     3841 cccagtagcc atgttgcaag actacaaata ttcagtgtag aaggacaaaa ggcaattcag
     3901 atcaaacatc aggatgaggt taattggata gcgggtgata ttatgcataa tcttattttt
     3961 caaatgtatg atgaaggaga aagagaaatc aatataacat cagctttagc agaaaaaatt
     4021 aaagttaatt ggactcctga gattaacaaa gaacacttgc tacagggtct gcttcctgat
     4081 gtgcaagtac caacatctgt aaaagatatg cgctattgcc aggtttcatt ccaagatgat
     4141 catgtgtctt tggaaagtgc gtttacagta agaccacttc ctgatgaacc taaacattta
     4201 aaatgtgaaa tgaaaggagg aaaaacagta cagatgggcc aagagcttca aggagaagta
     4261 gttataataa ttacagatca gtacggaaat cagattcaag cattttcacc aagttcttta
     4321 tcttctttgt caattgctgg ggttggactt gatagctcaa atttgaaaac aacctttcag
     4381 gaaaacacac agagtataag tgtaagaggc atcaaattta ttccaggtcc tcctggaaat
     4441 aaggatcttt gttttacttg gcgtgagttt tctgacttta ttcgagtgca actaatttct
     4501 ggacctcctg ctaaacttct ccttatagac tggccagaac taaaggagtc cattccagtg
     4561 attaatggaa gagatttaca gaaccctatt attgttcaac tttgtgatca gtgggataat
     4621 ccagcaccgg tacaacatgt taaaataagt cttacaaaag ctagcaattt aaagctcatg
     4681 ccttcaaacc aacagcataa aacagatgag aaaggcaggg ctaatttggg agtattcagt
     4741 gtttttgccc ctaggggaga gcatactctt caggttaaag ccatctataa caaaagtatc
     4801 atagaaggac ctataattaa gttaatgatt cttccagacc cagaaaaacc cgttcgtctc
     4861 aatgttaaat atgacaaaga tgcatccttc ttagcagggg gtcttttcac tgattttatg
     4921 attagtgtta tttctgaaga tgacagtatc attaaaaaca ttaatccagc acgtatttcc
     4981 atgaaaatgt ggaagctgtc taccagtggg aaccgacccc cagcaaatgc agaaacattt
     5041 agttgtaata aaataaaaga taatgacaaa gaagatggct gcttctattt cagggataaa
     5101 gtaattccta ataaagtggg gacatattgt atccagtttg gttttatgat ggataaaaca
     5161 aatattctca acagtgaaca ggttatagtt gaagtcctgc ctaatcaacc tgtgaagtta
     5221 gtacctaaaa ttaaaccacc tacaccagct gtttcaaatg ttcgctcagt tgccagtagg
     5281 accttggtca gagatctaca tcttagtatc acggactaca aagaccatga cggtgattat
     5341 aaagatcatg acatcgatta caaggatgac gatgacaagt gagtcgacaa tcaacctctg
     5401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5761 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5941 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     6001 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     6061 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     6121 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     6181 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     6241 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     6301 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     6361 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     6421 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     6481 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     6541 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6601 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6661 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6721 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6781 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6841 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6901 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6961 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7021 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7081 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     7141 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     7201 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     7261 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7321 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7381 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7441 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7501 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7561 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7621 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7681 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     7861 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     7921 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7981 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8041 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8101 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8161 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8221 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8281 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8341 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8401 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8461 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     8521 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8581 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8641 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8701 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8761 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8821 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8881 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8941 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9001 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9061 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9121 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9181 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9241 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9301 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9361 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9421 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9481 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9541 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9601 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9661 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9721 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaacta tttttccgaa
     9781 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9841 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9901 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9961 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10021 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10081 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10141 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10201 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10261 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10321 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10381 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10441 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10501 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10561 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10621 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    10681 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
    10741 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
    10801 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
    10861 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
    10921 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
    10981 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11041 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11101 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    11161 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    11221 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    11281 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    11341 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    11401 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    11461 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    11521 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    11581 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    11641 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    11701 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    11761 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    11821 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    11881 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    11941 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    12001 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    12061 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    12121 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    12181 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    12241 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    12301 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    12361 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    12421 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    12481 agagacagat ccattcgatt agtgaacgga tc
//
