LOCUS       sequence-255554-       10040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2842..2907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2917..3505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2970..2990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3388..3399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3519..4018
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3554..3577)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3951..3970
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4039..4638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4039..4058)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4535..4555
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4776..5009
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5081..5202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5118..5137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5172..5191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5242..5377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5397..5416)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5398..5416)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5426..5443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5426..5442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5435..5457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5584..6039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5671..5690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5881..5902
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6065..6169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6170..6238,6239..7030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6388..6407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7201..7789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7690..7709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7943..7960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8077..8098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8113..8118,8119..8136,8137..8143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8151..8167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8156..8178
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8175..8191
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8175..8191
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8210..8230
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8212..8230
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8258..8484
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8485..8665
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8712..8837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9330..9563
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9748..9792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaagt gccacacatt gaagcacttc tgaaaagaaa gctatcagaa
     1921 caagaagaac tgaagaaaaa acctagaaga tcgtgtactc ttccaaacta tactaaaggc
     1981 agtggagatg ttttgggaaa gattgcacat ctagcacaaa ttgaagatga tagagctgcg
     2041 atggttattt cttggcatct ggcaagtgac atggactgtg tagtcaccct aaccactgac
     2101 gctgcacgtc gtatctatga tgaaacccaa ggtcgtcagc aggtgttgcc ccttgattct
     2161 atttacaaga agactcttcc agattggaaa agatctctac ctcatttccg aaatggaaaa
     2221 ttgtatttta aacccattgg agatccagtc tttgctcgag acttgttaac atttccagat
     2281 aatgtagaac attgtgaaac agtatttggt atgctgttag gagacaccat tattttggat
     2341 aatctggatg cggccaatca ttatagaaaa gaggttgtta aaattacaca ctgtcctaca
     2401 ctgctgacca gagatggaga tcgaattcga agtaatggaa agtttggggg ccttcagaat
     2461 aaagctcctc caatggataa acttcgggga atggtatttg gagctccagt tccaaaacag
     2521 tgtctgatct taggggaaca aatagatctt cttcagcagt atcgttctgc tgtgtgcaaa
     2581 ctagacagtg tgaataagga tcttaacagt caattagagt accttcgcac tccggatatg
     2641 aggaagaaaa agcaagaact tgatgaacat gagaaaaatc tcaaactaat agaggaaaaa
     2701 ctaggtatga ctcccatacg taagtgtaat gactcattgc gtcattcacc aaaggttgag
     2761 acgacagatt gtccagttcc tcctaaaaga atgagacgag aagctacaag acaaaatagg
     2821 attataacca aaacagatgt agactacaaa gaccatgacg gtgattataa agatcatgac
     2881 atcgattaca aggatgacga tgacaagtga gtcgacaatc aacctctgga ttacaaaatt
     2941 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3001 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3061 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3121 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3181 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3241 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3301 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3361 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3421 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3481 atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc
     3541 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     3601 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     3661 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     3721 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     3781 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     3841 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     3901 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     3961 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     4021 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4081 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4141 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4201 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4261 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4321 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4381 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4441 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4501 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4561 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4621 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     4681 cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt
     4741 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     4801 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4981 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5041 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5221 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5341 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5401 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5461 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5521 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5581 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5641 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5701 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5761 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5821 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5881 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5941 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6001 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8221 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     8281 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     8341 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     8401 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     8461 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     8521 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8581 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8641 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8701 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8761 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8821 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8881 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8941 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     9001 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     9061 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     9121 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     9181 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     9241 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     9301 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     9361 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     9421 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     9481 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     9541 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     9601 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9661 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9721 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9781 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9841 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9901 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9961 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    10021 attcgattag tgaacggatc
//
