LOCUS       sequence-255560-        9017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1819..1884
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1894..2482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1947..1967)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2365..2376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2496..2995
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2531..2554)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2928..2947
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3016..3615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3016..3035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3512..3532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3753..3986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4058..4179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4095..4114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4149..4168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4219..4354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4374..4393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4375..4393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4403..4420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4403..4419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4412..4434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4561..5016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4648..4667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4858..4879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5042..5146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5147..5215,5216..6007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5365..5384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6178..6766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6667..6686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6920..6937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7054..7075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7090..7095,7096..7113,7114..7120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7128..7144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7133..7155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7152..7168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7152..7168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7187..7207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7189..7207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7235..7461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7462..7642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7689..7814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8307..8540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8725..8769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gatgactacg acaaccatac tggaattgat
      781 ttggttggca ctataatagc caccattaaa ggctctaatg aggaagatac tgatacccca
      841 ctttttattg ggaaagttag aacacttgaa ttccccttcg tgaatggttc ggctgaaatc
      901 atgagtctgg tgctggcaga aagtagtcct ggaagggata gtactgaata ttttattgta
      961 tttgagcccc ggctaccact tttatcaaga accttagaac catatatcct accgttcatg
     1021 ttttacaatg atgttaagaa gcagcaacaa atggcagcac ttacaaaaga aaaggaccaa
     1081 ttatctcagt ctattgttat gtataaaagt ttatttgaag ccagccaaca gcttcttaat
     1141 gaaatgaaat gtcaagttga agaagcaaga ttaaaagagg cccaattgcg aaatgaacta
     1201 aaaatacata atattgacat tcctacaaca caacaggtgc cacacattga agcacttctg
     1261 aaaagaaagc tatcagaaca agaagaactg aagaaaaaac ctagaagatc gtgtactctt
     1321 ccaaactata ctaaaggcag tagaaaagag gttgttaaaa ttacacactg tcctacactg
     1381 ctgaccagag atggagatcg aattcgaagt aatggaaagt ttgggggcct tcagaataaa
     1441 gctcctccaa tggataaact tcggggaatg gtatttggag ctccagttcc aaaacagtgt
     1501 ctgatcttag gggaacaaat agatcttctt cagcagtatc gttctgctgt gtgcaaacta
     1561 gacagtgtga ataaggatct taacagtcaa ttagagtacc ttcgcactcc ggatatgagg
     1621 aagaaaaagc aagaacttga tgaacatgag aaaaatctca aactaataga ggaaaaacta
     1681 ggtatgactc ccatacgtaa gtgtaatgac tcattgcgtc attcaccaaa ggttgagacg
     1741 acagattgtc cagttcctcc taaaagaatg agacgagaag ctacaagaca aaataggatt
     1801 ataaccaaaa cagatgtaga ctacaaagac catgacggtg attataaaga tcatgacatc
     1861 gattacaagg atgacgatga caagtgagtc gacaatcaac ctctggatta caaaatttgt
     1921 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1981 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2041 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2101 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2161 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2221 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2281 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     2341 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2401 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2461 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     2521 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     2581 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     2641 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     2701 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     2761 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     2821 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     2881 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     2941 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     3001 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3061 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3121 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3181 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3241 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3301 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3361 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3421 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3481 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     3541 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3601 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     3661 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     3721 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     3781 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3841 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3901 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3961 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     4021 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     4081 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4141 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     4201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4321 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     4381 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4441 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4621 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4861 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4921 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4981 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     5041 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5101 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5161 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5221 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5281 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5341 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5401 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5461 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5521 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5581 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5641 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5701 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5761 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5821 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5881 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5941 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6001 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6061 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6121 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6181 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6241 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6301 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6361 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6421 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6541 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6781 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6841 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6901 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6961 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7021 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7081 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7141 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     7201 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     7261 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     7321 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     7381 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     7441 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     7501 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7561 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7621 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7681 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7741 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7801 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7861 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7921 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7981 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8041 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     8101 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     8161 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     8221 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     8281 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     8341 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     8401 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     8461 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8521 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8581 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8641 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8701 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8761 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     8821 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8881 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     8941 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     9001 cgattagtga acggatc
//
