LOCUS       sequence-255648-       10706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 
            promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for 
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH
  TITLE     Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA 
            integration.
  JOURNAL   Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. 
            Epub 2019 Jun 12.
  PUBMED    31189177
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1323-z"; journalName: "Nature"; date: "2019-07";
            volume: "571"; issue: "7764"; pages: "219-225"
COMMENT     Sequence Label: pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2665..2684
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2768..2787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2875..2897
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2917..2946
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2956..4056
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4030..4053
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4111..4131
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(9003..9021)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9017..9064
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9430..9435,9436..9452,9453..9458))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(9485..9503)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9571..9675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9676..9744,9745..10536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9894..9913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc
     2821 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
     2881 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatcaccat
     2941 caccatggtt cttctatgaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
     3001 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
     3061 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
     3121 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
     3181 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
     3241 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
     3301 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
     3361 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
     3421 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
     3481 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
     3541 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
     3601 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
     3661 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
     3721 tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag
     3781 ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
     3841 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
     3901 ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
     3961 cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt
     4021 cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa caacaacaac
     4081 aataacaata acaacaacct cgggatcgag gaaaacctgt acttccaatc caatgcaatg
     4141 tttttgcaaa gacctaaacc ttacagcgat gaaagtttag aaagtttctt tatccgagtg
     4201 gctaacaaaa atggctacgg tgatgtccat cgcttcctag aagccactaa acgattcctt
     4261 caagacattg accataatgg ctatcaaacc tttccgactg atataactcg gataaaccca
     4321 tactcagcta aaaacagttc cagcgcacga actgcgtcat tcctgaaact tgcacaattg
     4381 acatttaatg aaccgccaga gctacttggg ttggcaatta acagaacaaa catgaaatac
     4441 tcgccgtcaa ctagcgcggt tgttagaggt gcagaagtct ttcctcgcag tttactacgg
     4501 acgcactcca tcccctgctg tcctttgtgt ctgcgagaaa atggctacgc ctcctacctt
     4561 tggcactttc aggggtacga atactgccac agccataacg tacctttaat taccacttgt
     4621 agctgtggta aggagtttga ctaccgagta tctgggttaa agggcatttg ctgcaaatgc
     4681 aaggagccta tcaccttaac cagcagggag aacggtcatg aggcagcgtg tactgtttca
     4741 aactggcttg ctggccatga atctaaacct ctgccaaatc ttcctaaaag ctaccgatgg
     4801 ggtttagttc attggtggat gggtattaaa gatagcgagt tcgatcactt ttcgttcgtt
     4861 caatttttct caaactggcc aaggtcattc cactcgataa tcgaagatga agtagagttc
     4921 aaccttgagc atgctgttgt cagcacgtct gaattacgac taaaagatct tcttggtcga
     4981 ttgtttttcg gttcaattcg gttacctgag cggaatcttc aacacaatat catccttggt
     5041 gagcttctct gctatttaga aaatcgttta tggcaagaca agggattaat cgccaacctc
     5101 aaaatgaacg cgttagaggc gactgtaatg ttaaattgta gcctcgatca gattgcatca
     5161 atggttgaac aacgcatctt gaagccaaat cgaaaaagca agcccaacag ccctcttgat
     5221 gttaccgatt atctatttca tttcggcgat attttctgtc tttggttagc tgagttccaa
     5281 agcgatgagt ttaaccgttc gttttatgtg tcgaggtggt aaatgcaaac tctgaaagaa
     5341 ctaatcgcat ccaatcctga cgacttaaca actgaactta agagagcatt tcgtccactc
     5401 acaccgcata ttgcaattga tggtaatgaa cttgacgcac tgacgatatt agtcaattta
     5461 accgataaga ctgatgatca gaaagacctg ctcgatcgag ccaaatgcaa gcaaaaactt
     5521 cgagatgaaa aatggtgggc tagctgcata aattgcgtta attacagaca aagccataac
     5581 ccaaaattcc cggatatacg ttctgaaggc gtgattcgaa cccaagccct gggtgaatta
     5641 ccgagtttcc tactctcgtc ctcaaaaatc ccaccatacc attggtcata tagccatgat
     5701 tcaaaatacg tcaacaaaag cgcattcctc accaatgagt tttgttggga tggtgagatc
     5761 tcatgtttgg gtgagcttct taaagatgca gatcacccac tttggaatac tttgaaaaag
     5821 ttaggttgtt ctcaaaaaac ttgcaaagca atggcaaaac aactagctga tattactctc
     5881 acgactatca atgtcacact cgcaccaaat tacctgactc aaatatctct ccccgatagt
     5941 gatacatcct acatttcact ctctcctgtt gcatcgctat cgatgcaaag ccactttcat
     6001 cagaggctcc aagatgaaaa tcggcatagc gcgataacgc ggtttagccg aactaccaac
     6061 atgggggtca ctgcgatgac atgtggcggc gcatttagaa tgttaaagtc tggcgctaag
     6121 ttttccagcc cccctcatca ccgattaaac agtaaacgaa gttggttgac gtcagagcat
     6181 gttcagtcat taaaacagta ccagcgcctc aacaaaagcc tcatacctga aaactctcgg
     6241 attgcactcc gtagaaaata caaaatcgag cttcaaaata tggtcagatc ttggtttgca
     6301 atgcaagacc atacccttga ttcgaacata cttatccaac atttgaatca tgacctatct
     6361 tacttaggag ccacaaaacg ttttgcatac gatccagcga tgaccaagct ctttactgag
     6421 cttttgaaac gagagttatc aaattcaatc aataatggtg agcaacacac taatggatcg
     6481 tttttagtcc taccgaatat cagggtttgt ggcgcaacag ctttaagctc cccggtaacg
     6541 gtggggattc catcacttac agctttcttt ggcttcgttc acgcatttga acggaatata
     6601 aatcgcacca cctcatcgtt tcgtgttgaa tcctttgcga tatgcgtcca tcaactacat
     6661 gtcgagaagc gaggtttgac agcagagttt gtggaaaaag gcgacgggac tatatccgct
     6721 cccgcgaccc gggatgactg gcagtgtgat gtcgtattta gccttatttt gaacaccaac
     6781 tttgctcaac atattgacca agatacgtta gttacatcac taccaaagcg attggctcgg
     6841 ggttcagcaa aaattgcgat tgatgacttt aaacatatca actcattctc gacattagaa
     6901 acagcgatcg aatctctgcc aatagaagct ggtaggtggt tatcacttta cgcacagtca
     6961 aacaataatc taagtgatct attagcagcc atgacagagg accatcagct catggcaagc
     7021 tgcgtcggtt accacttgtt agaagagccc aaagataaac caaactccct cagaggttac
     7081 aaacacgcta tcgccgagtg catcattgga ctcattaact caatcacctt tagctcagag
     7141 actgatccca acacaatctt ttggtcgcta aagaactatc aaaactacct agtggtacag
     7201 ccaaggagta tcaacgatga aactaccgac aaatctagcc tatgagcgct ctatcgaccc
     7261 atcagatgtc tgtttttttg tcgtctggcc cgatgataga aaaacacctt taacctacaa
     7321 ttctcgtact ctgctcgggc aaatggaagc ggcatcatta gcctatgatg tctcaggtca
     7381 accaataaaa agtgccaccg ctgaggcgtt agctcaaggg aaccctcatc aagttgattt
     7441 ctgccacgtt ccatacggtg cgagtcatat tgaatgcagt ttctccgtct cgttttcttc
     7501 tgaactacgt caaccatata agtgtaactc aagcaaagtt aaacaaacgc tagtgcaatt
     7561 agtcgagctc tacgaaacga aaatcggctg gactgagcta gcaacccgat atttgatgaa
     7621 catttgcaac ggtaaatggc tgtggaaaaa tacccgtaaa gcctattgct ggaacattgt
     7681 acttacacct tggccttgga acggggaaaa ggttggattt gaagatattc gtactaacta
     7741 cacctcacgg caagacttta aaaataataa aaattggtct gctatagttg aaatgatcaa
     7801 aaccgcattt tctagtactg atgggctggc gatatttgaa gtcagggcca ccttgcactt
     7861 gccaacgaat gctatggtgc ggccaagcca agttttcaca gaaaaagaaa gtggcagtaa
     7921 aagtaaatct aaaactcaaa acagtcgagt ttttcagagt acaactattg atggtgaacg
     7981 atcgccaata ctaggggcct ttaaaacggg agcagctatt gcaaccattg acgactggta
     8041 tcctgaagcc actgagccac taagggtcgg acggtttggg gttcatcgcg aagatgtcac
     8101 ttgctaccgt catccgtcta ccggaaaaga ttttttctcg atattacaac aagcagagca
     8161 ctatattgaa gtgttgagcg ccaacaaaac tcccgctcaa gaaactatca acgacatgca
     8221 ctttttaatg gctaacctga ttaagggtgg gatgttccag cataaaggag actgactgtg
     8281 aaatggtatt ataagacaat cacctttctg ccagagttgt gcaacaacga gtcactggct
     8341 gcaaagtgtc tccgcgttct gcatggattt aactatcagt atgagacacg aaatatcggc
     8401 gtttcatttc cgctttggtg tgatgcaacg gttggaaaaa agatttcatt tgtcagcaag
     8461 aacaagatag aactcgactt actacttaaa caacactatt tcgtccaaat ggaacaactt
     8521 caatactttc atatatccaa cactgttctc gtcccagaag attgtacata cgtttccttt
     8581 agacgctgtc aatctataga taagctcaca gcagcagggc tggcaaggaa aatcagacgc
     8641 ctggagaaac gtgctctatc tagaggcgag caatttgacc catcatcttt tgctcaaaaa
     8701 gagcatactg caatagcgca ctaccactca cttggggagt ccagcaaaca gacgaaccgc
     8761 aactttcgac tcaatatcag gatgctctcg gagcaacccc gtgaggggaa ctcgattttt
     8821 agtagctatg gcttatcaaa ttcagaaaac tcgtttcagc ctgtaccctt aatctaatag
     8881 cattggaagt ggataacgga tccgcgatcg cggcgcgcca cctggtggcc ggccggtacc
     8941 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     9001 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     9061 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
     9121 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
     9181 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
     9241 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
     9301 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
     9361 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
     9421 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     9481 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     9541 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataacat
     9661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9901 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     9961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10201 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10681 cccgtagaaa agatcaaagg atcttc
//
