LOCUS       sequence-255663-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Golgi apparatus marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(242..816,817..819,820..828)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(409..426)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     636..655
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(848..867)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cggatggcgc gggacctctc ggcatggacg agctgtacaa gtactcagat ctcgacccca
       61 aagctgctag ttcaattgat cagtttagta ttcgcctggg aatttttctc cgaagatacc
      121 ccatagcgcg agtttttgta attatatata tggctttgct tcacctctgg gtcatgattg
      181 ttctgttgac ttacacacca gaaatgcacc acgaccaacc atatggcaaa tgaggatccc
      241 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
      301 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
      361 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
      421 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
      481 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
      541 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
      601 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
      661 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
      721 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
      781 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg
      841 accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
      901 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gcacgcgcca caccgtcgac
      961 cggacgcaca tcgagcggtc accgagctgc agactcttct cacgcgcgtc ggctcgacat
     1021 cgcaaggtgt gggtcgcgac gacggcgccg tgcgtctgaa cagccgaacg taaccggacg
     1081 tgatcacgaa tcgacgccat gcgagttcga tcgactgcca cgacatgaag ctctgaagct
     1141 gtgcaggatc gagatagtcg tcgctgcgac acagggcaga gttggaatat gcaa
//
