LOCUS       sequence-25571-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(206..226)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(206..224)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(245..261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(245..261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(258..280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tagatgngtg tttgagacta taaatatccc ttggagaaaa gccttgtttg caatgctgac
       61 gactttcaaa gattcaagag atcattgaaa gtcgtcagca ttgctttttt aattctttgt
      121 taggcctggt acctctagag atatcctgca gtggccaatg cattggttcg cggccgccac
      181 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cgggccctgc catagcctca
      301 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      541 caagagctac caactctttt tccgaaggta actggcttca gcagagcnac anancccaaa
      601 tactgttctt ctagtgtagc cgtagttagg ccaccacctg caagaactct gtagcaccgc
      661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      721 gtcttaccgg gttggactcc agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      781 cgggggntcg tgc
//
