LOCUS       sequence-255747-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF K483E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-K483E
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5750..5774)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5827..5868
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5889..6456
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5906..6458
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6050..6067)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6277..6296
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6470..6472,6473..6475,6476..7189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6470..6472,6473..6475,6476..7186)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6515..6536)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6776..6795)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7123..7144
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7203..7791
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7256..7276)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7674..7685)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7866..8099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8218..8236)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8304..8408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8409..8477,8478..9269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8627..8646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9440..10028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9929..9948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10182..10199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10439..10460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10475..10480,10481..10498,10499..10505)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10513..10529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10518..10540
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10537..10553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10537..10553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10603..10622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcgctga gcggtggcgg tggtggcggc
     3481 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc
     3541 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa
     3601 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag
     3661 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca
     3721 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt
     3781 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta
     3841 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca
     3901 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca
     3961 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc
     4021 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac
     4081 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca
     4141 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt
     4201 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt
     4261 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc
     4321 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc
     4381 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc
     4441 accagtccgt ctccttcaaa atccattcca attccacagc ccttccgacc agcagatgaa
     4501 gatcatcgaa atcaatttgg gcaacgagac cgatcctcat cagctcccaa tgtgcatata
     4561 aacacaatag aacctgtcaa tattgatgac ttgattagag accaaggatt tcgtggtgat
     4621 ggaggatcaa ccacaggttt gtctgctacc ccccctgcct cattacctgg ctcactaact
     4681 aacgtgaaag ccttacagaa atctccagga cctcagcgag aaaggaagtc atcttcatcc
     4741 tcagaagaca ggaatcgaat gaaaacactt ggtagacggg actcgagtga tgattgggag
     4801 attcctgatg ggcagattac agtgggacaa agaattggat ctggatcatt tggaacagtc
     4861 tacaagggaa agtggcatgg tgatgtggca gtggaaatgt tgaatgtgac agcacctaca
     4921 cctcagcagt tacaagcctt caaaaatgaa gtaggagtac tcaggaaaac acgacatgtg
     4981 aatatcctac tcttcatggg ctattccaca aagccacaac tggctattgt tacccagtgg
     5041 tgtgagggct ccagcttgta tcaccatctc catatcattg agaccaaatt tgagatgatc
     5101 aaacttatag atattgcacg acagactgca cagggcatgg attacttaca cgccaagtca
     5161 atcatccaca gagacctcaa gagtaataat atatttcttc atgaagacct cacagtaaaa
     5221 ataggtgatt ttggtctagc tacagtgaaa tctcgatgga gtgggtccca tcagtttgaa
     5281 cagttgtctg gatccatttt gtggatggca ccagaagtca tcagaatgca agataaaaat
     5341 ccatacagct ttcagtcaga tgtatatgca tttggaattg ttctgtatga attgatgact
     5401 ggacagttac cttattcaaa catcaacaac agggaccaga taatttttat ggtgggacga
     5461 ggatacctgt ctccagatct cagtaaggta cggagtaact gtccaaaagc catgaagaga
     5521 ttaatggcag agtgcctcaa aaagaaaaga gatgagagac cactctttcc ccaaattctc
     5581 gcctctattg agctgctggc ccgctcattg ccaaaaattc accgcagtgc atcagaaccc
     5641 tccttgaatc gggctggttt ccaaacagag gattttagtc tatatgcttg tgcttctcca
     5701 aaaacaccca tccaggcagg gggatatggt gcgtttcctg tccactagaa cccagctttc
     5761 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     5821 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag
     5881 taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5941 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6001 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6061 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6121 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6181 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6241 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6301 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6361 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6421 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata tggtgagcaa
     6481 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6541 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6601 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6661 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6721 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6781 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6841 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6901 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6961 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     7021 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     7081 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     7141 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa tcgatagatc
     7201 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7261 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7321 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7381 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7441 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7501 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7561 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7621 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7681 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7741 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     7801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7861 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8101 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8161 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8221 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8401 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8461 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8521 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8581 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8641 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8701 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8761 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8821 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8881 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8941 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9001 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9061 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10081 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10141 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10201 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10261 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10381 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10441 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10501 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    10561 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10621 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10681 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10741 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10801 caaacttagt agt
//
