LOCUS       sequence-255858-       10685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast transcriptional reporter of aggregating proteins (yTRAP) to 
            detect protein aggregation, Gateway backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pGAN147
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    77..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     82..104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     101..117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     101..117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1610..1734
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1759..1764,1765..1782,1783..1789)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1843..2502
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1909..1928)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2844..3149
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3028..3047
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3190..3314)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3352..3372
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3976..4065
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4072..4161
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      5298..5307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      5774..5961
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5829..5853)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6036..7167
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     CDS             6386..6958
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      6970..7167
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     7730..7746
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7730..7746
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7760..7779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7761..7779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7786..7803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7786..7802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7795..7817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8102..8121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8234..8255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8409..8428)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8528..8550
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8588..8606)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8674..8778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8779..8847,8848..9639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8997..9016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9810..10398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10299..10318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10552..10569
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccgcggct
       61 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      121 gattacgcca ctagtccgag gcctcgagat ccgatatcgc cgtggcggcc gcaattatcc
      181 tgggcacgag tgaaacaaag ctaaaacctt tatttagcat ggccattgaa tgtaacaatt
      241 atatatatcg caagcacaaa aaatcaagga gagagaacta ccactttgtt catgtgtaca
      301 atgttcatta tctccataag caaaaaaaaa aaaatagaaa acatatgcta taaggttgat
      361 attctcacga gtaagcggca cttgctactt attgacattg cagatttttg gctacagaaa
      421 tagtatatta gagattataa ttgctaatca aatcaaaata taaaattagt aaaccaaacc
      481 atttataccc ttccttagta gttatggatt gttttttaat gatatttctg caaaccaaag
      541 aaagattgtt atccagatag aatttagttt tgatattcat ttttttgttg aagattgaac
      601 gccatatctg ggcctcataa ttcaaaagac ggtgccatta tcggtagcgt ttcgcattgt
      661 actggatttc agaaatttca cagttgatga atcgaaaaga atggtctcat tgcaacacgt
      721 aaggttaaga tgtccctttt taccattata ggcaataaat gaatcataaa acgaccgtat
      781 actggtgaaa tagtagggag aacgagtacc tgtagtaaaa agtataaatc atagttaatc
      841 gggcaatgtc cctcgatcaa ggagtattgt gtcatgttcg agacaaacgc caacattttt
      901 gtttcttttg gacaaatgtt gtttgcattt atgatccgtt atattttgat ctaatgtaga
      961 gttgcacgta gttcttactg gcaaagaaat cgatgcatac caaaaaagaa taaaggtgat
     1021 atttgatctt taccgtttag ttccaacgta aaattgtgcc tttggactta aaatggcgtc
     1081 gtacgctgca ccgagtgcaa ccacacaaaa atcatacaac gaatggtata tgcttcattt
     1141 ctttgtttcg cattagctgc gctatttgac tcaaattatt attttttact aagacgacgc
     1201 gtcacagtgt tcgagtctgt gtcatttctt ttgtaattct cttaaaccac ttcataaagt
     1261 tgtgaagttc atagcaaaat tcttccgcaa aaagatgaat cttagttctc agcccaccaa
     1321 aagaggtaca tgctaagatc atacagaagt tattgtcact tcttaccttg ctcttaaatg
     1381 tacattacaa ccgggtatta tatcttacat catcgtataa tatgatcttt ctttatggag
     1441 aaaatttttt tttcactcga ccaaagctcc cattgcttct gaagagtgta gtgtatattg
     1501 gtacatcttc tcttgaaaga ctccattgta ctgtaacaaa aagcggtttc ttcatcgact
     1561 tgctcggaat aacatctata tctgcccact agcaacagct agccccatca caagtttgta
     1621 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1681 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     1741 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1801 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1861 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1921 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1981 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2041 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2101 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2161 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2221 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2281 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2341 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2401 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2461 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2521 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2581 actgatatgt atacccgaag tatgtcaaaa agaggtatgc aatgaagcag cgtattacag
     2641 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2701 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2761 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2821 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2881 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2941 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3001 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     3061 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3121 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3181 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3241 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3301 ttgtacaaag tggtgatggg ctgcaggaat tcgatatcaa gcttaatggc tccaaagaag
     3361 aagagaaagg ttggtattca tggtgttcca gttgatggtg gtttacaagc tccaccaact
     3421 gatgtttctt tgggtgatga attgcatttg gatggtgaag atgttgctat ggctcatgct
     3481 gatgctttgg atgattttga tttggatatg ttgggtgacg gtgattctcc aggtccaggt
     3541 tttactccac atgattctgc tccatatggt gctttagata tggctgactt tgaattcgaa
     3601 caaatgttca ccgatgcctt gggtattgat gaatatggtg gtggtgctag accaggtgaa
     3661 agaccatttc aatgtagaat ctgcatgaga aacttctcca gacaagatag attggataga
     3721 cataccagaa ctcatactgg tgaaaagcca ttccaatgca gaatatgtat gagaaatttt
     3781 tcccaaaaag aacacttggc cggtcatttg agaacacaca caggtgaaaa accttttcaa
     3841 tgcagaatat gcatgagaaa ctttagtaga agagacaact tgaacagaca cttgaaaacc
     3901 catttgagag gttctgccgg cgaattggct ttgaaattgg ctggtttgga tatcaattct
     3961 ggttctgctg ctagataccc atatgatgtt ccagattatg ctggttaccc ttacgatgtt
     4021 cctgactacg caggttctta tccatacgat gtacctgatt atgcctcaag atatccttat
     4081 gacgtccctg attacgccgg ttatccatat gacgtaccag actacgctgg tagttaccca
     4141 tacgacgtcc cagattacgc ttcttcataa tgagggccct catgtaattt cttgcaaaca
     4201 taagtaaatg caaacacaat aataccgatc ataaagcatt ttcttctata ttaaaaaaca
     4261 aggtttaata aagctgttat atatatatat atatatatag acgtataatt agtttagttc
     4321 tttttgtacc atataccata aacaaggtaa acttcacctc tcaatatatc tagaatttca
     4381 taaaaatatc tagcaaggtt tcaactcctt caatcacgtt ttcatcactc aaccctatct
     4441 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4501 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct
     4561 gatgcggtat tttctttaat taaagagtga ggactcgaaa atattaatag agtgaggact
     4621 cgaaaatatt aatagagtga ggactcgaaa atattaatag agtgaggact cgaaaatatt
     4681 aatatcgata gagtgaggac tcgaaaatat taatagagtg aggactcgaa aatattaatg
     4741 aattcagagt gaggactcga aaatattaat agagtgagga ctcgaaaata ttaatggccg
     4801 gcccagatcc gccaggcgtg tatatatagc gtggatggcc aggcaacttt agtgctgaca
     4861 catacaggca tatatatatg tgtgcgacga cacatgatca tatggcatgc atgtgctctg
     4921 tatgtatata aaactcttgt tttcttcttt tctctaaata ttctttcctt atacattagg
     4981 acctttgcag cataaattac tatacttcta tagacacaca aacacaaata cacacactgt
     5041 ttaaacaaaa aatgggcgag gaggataaca tggcctctct cccagcgaca catgagttac
     5101 acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc
     5161 caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc
     5221 cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg
     5281 ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa
     5341 tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc
     5401 acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga
     5461 ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa
     5521 ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca
     5581 ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc
     5641 cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg
     5701 agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag taaggcgcgc
     5761 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     5821 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     5881 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
     5941 tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat agatctgttt
     6001 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
     6061 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     6121 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     6181 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     6241 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     6301 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     6361 catccgaaca tcaattgaaa caaccatggg taccactctt gacgacacgg cttaccggta
     6421 ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac
     6481 cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt
     6541 ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgacgg
     6601 ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc
     6661 gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga
     6721 ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga
     6781 gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc
     6841 ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta
     6901 cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctaaag
     6961 atcttcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     7021 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     7081 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
     7141 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg
     7201 tcgaaaacga gctcgaattc ctgggggaac aacttcacag aatgttttgt catattgtcg
     7261 aagtggtcac aaaacaagag aagttccgcc aattataaaa agggaacccg tatatttcag
     7321 cttcacggat gatttccagg gtgagagtac tgtatatggg cttacgatag aaggccataa
     7381 aaatttcttg cttggcaaca aaatagaagt gaaatcatgt cgaggctgct gtgtgggaga
     7441 acagcataaa atatcacaaa aaaagaatct aaaacactgt gttgcttgtc ccagaaaggg
     7501 aatcaagtat ttttataaag attggagtgg taaaaatcga gtatgtgcta gatgctatgg
     7561 aagatacaaa ttcagcggtc atcactgtat aaattgcaag tatgtaccag aagcacgtga
     7621 agtgaaaaag gcaaaagaca aaggcgaaaa attgggcatt acgcccgaag gtttgccagt
     7681 taaaggacca gagtgtataa aatgtggcgg aatcttacag tgcggccgct ctagaactag
     7741 tggatcgatc cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     7801 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7861 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7921 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7981 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     8041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     8101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8161 ctaaatcggg gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8221 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
     8281 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
     8341 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     8401 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     8461 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8521 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     8581 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8641 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8701 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8761 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8821 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8881 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8941 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9001 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9061 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9121 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9181 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9241 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9301 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9361 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9421 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9481 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9541 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9601 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9661 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9721 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9781 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9901 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9961 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10021 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10081 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10141 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10201 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10261 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10321 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10381 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10441 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10501 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10561 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10621 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10681 gcaat
//
