LOCUS       sequence-25587-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pCAG-EGFP/RFP-miR-E2-4int
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           327..341
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aaanatcgng gaacanncga gcgcaccgag ggccgccacc acctgtttcc tgtaattaat
       61 taacaggtaa gtgcgatcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
      121 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
      181 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
      241 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggt acctgctgtt gacagtgagc
      301 gagaagtggt gtatctagag attatagtga agccacagat gtataatctc tatacaccac
      361 ttcctgccta ctgcctcgga cttcaagggg aattcagggt ggggccacct gccggtaggt
      421 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
      481 aatcgacagg cgccggacct ctggcggccg caacaacgcg ttcctgacca ttcatcctct
      541 ttctttttcc tgcaggcttg tggaagaaat gggaccctgc tgctgctgga gacggccact
      601 gctccctgag tgtcccgtcc ctggggctcc tgcttcngcn ctccngnaat tctcctgctc
      661 cccacctcct gnngtcctgc tgctcaccaa tcgctcnnga tccncccnan natnnnaaaa
      721 atttggggnn cnngccnang aaaccttccc cnccngnggc cnntgggnan ngaaccanaa
      781 aaacaacnnt gccctngntt gtttngaatc cnacccnacc ca
//
