LOCUS       sequence-256250-       12495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Richards KS, Swensen AM, Lipscombe D, Bommert K
  TITLE     Novel CaV2.1 clone replicates many properties of Purkinje cell 
            CaV2.1 current.
  JOURNAL   Eur J Neurosci. 2007 Nov . 26(10):2950-61.
  PUBMED    18001290
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Neurosci. 2007 Nov . 26(10):2950-61."
COMMENT     Sequence Label: CaV2.1 (pcDNA6)
FEATURES             Location/Qualifiers
     source          1..12495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7238..7267
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             7241..7270
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             8126..8167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8177..8194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8217..8234)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8223..8447
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8493..8921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8580..8599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8790..8811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8930..8950)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8935..9264
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9115..9250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9312..9359
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9378..9776
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9934..10055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9971..9990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10025..10044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10104..10120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10104..10120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10117..10139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10128..10144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10152..10158,10159..10176,10177..10182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10197..10218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10335..10352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10506..11094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10586..10605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11265..12056,12057..12125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11888..11907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12126..12230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12305..12324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcg atccactagt ccagtgtggt
      721 ggaattcggc ttgaggccct ttgctctttg cagaatggcc cgctttggag acgagatgcc
      781 gggccgctac ggcgcaggcg gaggaggctc agggccggcc gccggggtgg tcgtgggcgc
      841 cgcgggcggc cgaggagccg ggggcagccg gcagggcggg cagcccggag cgcagaggat
      901 gtacaagcag tcgatggcgc agagagcgcg gaccatggcc ctctacaacc ccatccctgt
      961 ccgccagaac tgcctcacgg tcaaccgctc cctgttcctc ttcagtgaag acaacgtggt
     1021 gagaaaatac gccaaaaaga tcacggaatg gcctcccttc gagtacatga tcctggccac
     1081 catcattgct aactgcatcg tcctggccct ggagcagcac ctccctgatg atgacaagac
     1141 acccatgtct gagcggctgg atgacacgga accatacttc attggaatct tctgttttga
     1201 ggctggaatt aagatcgtgg ctcttggctt tgccttccac aaaggctcct acctgaggaa
     1261 tggctggaac gtcatggact ttgtcgtggt gctaacaggc atcttggcca ctgtcgggac
     1321 ggagtttgac ctacggacac tgagggcggt tcgtgtgctg cggccactca agctggtgtc
     1381 tggaatccca agtttacaag tcgtcctgaa gtcaatcatg aaggcgatga tccctctgct
     1441 gcagatcggc ctcctcctgt tttttgcaat ccttattttt gcaatcatag ggttagaatt
     1501 ttatatgggg aaatttcata ccacctgctt tgaagagggg acagacgaca tccagggtga
     1561 gtcgccagct ccgtgtggga cagaggagcc tgcccgcacc tgccccaacg ggaccaaatg
     1621 tcagccgtac tgggaagggc ccaacaacgg catcactcag ttcgacaaca tcctgtttgc
     1681 tgtgctcact gttttccagt gcatcaccat ggaaggctgg actgatctcc tctacaatag
     1741 caacgatgcc tcagggaaca cttggaactg gttgtacttc atccccctca tcatcatcgg
     1801 ctcctttttt atgctgaacc ttgttctggg tgtgctgtct ggggagtttg ccaaagaaag
     1861 ggaacgtgtg gagaaccgga gagcttttct gaagctcaga agacaacagc agattgaacg
     1921 tgagctcaat ggatacatgg agtggatctc aaaagcagaa gaggtgattc ttgcggagga
     1981 cgagacagac gtggagcaga ggcacccttt tgatggagct cttcggagag ctactctgaa
     2041 gaaaagcaag acagacctgc tcaaccctga ggaggccgag gaccagctgg ctgacatcgc
     2101 gtctgtgggg tctcccttcg ccagagccag catcaaaagt gccaagctgg agaactcgac
     2161 tttttttcac aagaaggaga gaagaatgcg tttctacatc cgccgcatgg tcaaaactca
     2221 ggccttctac tggaccgtgc tcagtctggt agccctcaac acgctgtgcg tcgccattgt
     2281 ccactacaac cagcccgagt ggctctccga cttcctctac tatgcagaat tcattttctt
     2341 aggactcttt atgtccgaaa tgtttataaa aatgtacggg ctcgggacgc ggccttactt
     2401 ccactcttcc ttcaactgct ttgactgtgg ggtcatcatt gggagcatct ttgaagtcat
     2461 ctgggctgtc atcaaacccg gtacatcctt tggaatcagt gtgttacgag ctctcaggtt
     2521 attgcgaatt ttcaaagtca caaagtactg ggcatctctc agaaacctgg ttgtctccct
     2581 cctcaactcc atgaagtcca tcataagtct gttgttcctc cttttcctgt tcatcgttgt
     2641 ctttgccctc ctggggatgc agctgttcgg tggccaattt aattttgatg aggggactcc
     2701 gcccaccaac ttcgacactt ttccagcagc aataatgact gtgtttcaga tcctgactgg
     2761 cgaggattgg aatgaggtca tgtatgatgg gatcaagtct caggggggcg tgcagggcgg
     2821 catggtgttc tccatctact tcatcgtcct caccctcttc gggaactaca ccctgctgaa
     2881 cgtgttcttg gccatcgcag tggacaacct ggccaatgcc caggagctca ccaaggatga
     2941 gcaagaagag gaagaggcag ccaatcagaa gctggctcta cagaaagcca aggaggtagc
     3001 agaagtgagt cccctgtctg cagccaacat gtccatagcc gtgaaggaac agcagaagaa
     3061 ccagaagcct accaagtcgg tgtgggagca gcgcaccagc gagatgcgca agcagaatct
     3121 gctggccagc cgcgaggcgc tgtacgggga cgcggctgag cgctggccca ccccctacgc
     3181 gcgcccgctg cggcccgacg tgaagactca cttggaccgg ccgcttgtgg tggacccgca
     3241 ggagaaccgt aacaacaaca ccaacaagag ccgtgcgcca gaagcgctgc gcccgaccgc
     3301 gcggccccgc gagagcgcgc gcgaccccga cgcgcggcgc gcctggcccg gcagccctga
     3361 gcgcgcccct ggacgagagg gcccgtatgg gcgcgagagc gagcctcagc agcgcgatca
     3421 cgcgccgccc cgcgagcacg caccttggga cgcggacacg gagcgcgcca aagccgggga
     3481 cgcgccccgc cgccacacgc accgacctgt ggcggagggc gagcctcgcc gccaccgcgc
     3541 ccgccgccgg cccggggacg aacccgacga caggccagag cgcaggccgc gtccccgcga
     3601 cgccactagg ccggcccgcg ctgcagacgg cgaaggcgac gatggggagc gcaagcggcg
     3661 acaccgacac gggccgcccg cccacgacga cagggagcgc agacaccgac gcagaaaaga
     3721 gaaccagggc tctggggtcc ccgtgtctgg tcccaacttg tccaccacca ggccaatcca
     3781 gcaagacctg ggccgccagg acctgccgct ggctgaggat cttgacaaca tgaagaataa
     3841 caagctggcc accggggaac ctgccagtcc ccacgacagc ctgggccaca gcggccttcc
     3901 ccccagcccc gccaaaatcg ggaacagcac caaccctggt cccgccttgg ccaccaatcc
     3961 ccagaacgct gccagccgca ggacgcccaa caatccgggc aacccgtcca accccggccc
     4021 ccccaagact cccgagaaca gccttatcgt caccaacccc agcagcaccc agcccaactc
     4081 agcgaagact gccaggaaac ccgagcacat ggcggtggag atccccccag cctgcccgcc
     4141 cctcaaccac accgtggtcc aagtaaacaa aaacgccaac ccagacccac tgccaaagaa
     4201 agaggaagag aagaaagagg aagaggaggc agaccccggg gaggacggcc caaagcccat
     4261 gccgccctac agctccatgt tcatcctctc caccaccaac ccccttcgcc ggctgtgcca
     4321 ttacatcctg aacctgcgct acttcgagat gtgcatcctc atggtcattg ccatgagcag
     4381 catcgctctg gccgctgagg acccggtgca gcccaacgca ccccgcaaca atgtgctgcg
     4441 atattttgac tatgttttta caggcgtgtt tacctttgag atggtgatca agatgatcga
     4501 cctgggcctc gtcttgcatc agggggccta tttccgtgac ctatggaaca ttctggactt
     4561 catagtggtc agtggggccc tggtggcctt tgccttcact ggcaatagca aaggaaagga
     4621 cattaacacc attaagtctc tccgagtcct ccgggtgcta cgacctctaa agaccatcaa
     4681 gcggctgcca aagctgaagg ccgtgtttga ctgcgtggtg aactcgctca agaacgtctt
     4741 caacatcctc attgtgtaca tgctgttcat gttcatcttc gctgtggtag ccgtgcagct
     4801 cttcaagggc aagttcttcc actgcacgga cgagtccaag gagttcgaga gagactgtcg
     4861 aggcaagtac ctcctgtacg agaagaacga agtgaaggcc cgggaccgcg agtggaagaa
     4921 gtacgagttc cactacgaca acgtgctctg ggctctgctc acacttttca cagtgtccac
     4981 aggagagggc tggccacagg tcctcaagca ctcagtggat gctacttttg agaaccaggg
     5041 ccccagcccc gggtaccgca tggagatgtc catcttctac gtggtgtact ttgtagtgtt
     5101 tcccttcttc tttgtcaata tcttcgtggc cttgatcatc atcacctttc aggagcaggg
     5161 agacaagatg atggaagagt acagcctgga gaaaaatgag agggcctgca tcgactttgc
     5221 catcagtgcc aagccgctga ccaggcacat gccccagaac aagcagagct tccagtatcg
     5281 aatgtggcag ttcgtcgtgt ccccaccctt tgagtacacc atcatggcca tgatcgctct
     5341 caacaccatc gtgctaatga tgaagttcta cggagcctcg gtggcctatg aaaacgccct
     5401 tcgcgtgttc aacattgtct tcacctccct cttctctctc gaatgtgtgc ttaaagtcat
     5461 ggcttttggg attctgaatt atttccgcga tgcctggaac atcttcgact ttgtgactgt
     5521 tctgggcagc atcacagaca tcctcgtcac cgagtttggg aataacttca tcaacctgag
     5581 ctttctccgc ctcttccgtg ctgccagact catcaaactc ctccgccagg gttacaccat
     5641 ccgcattctc ctctggactt tcgtgcagtc cttcaaggcc ctaccttatg tctgtctgct
     5701 gatcgccatg ctcttcttca tctacgccat catcgggatg caggtgtttg gcaacatcgg
     5761 cattgacggg gaagacgagg acagcgacga ggatgaattc caaatcacgg agcacaataa
     5821 cttccggacc ttcttccaag cgctcatgct tctcttccgg agcgccacag gagaagcgtg
     5881 gcacaacatc atgctctcct gcctcagcgg gaagccgtgc gataagaact ctgggatcct
     5941 cacggcagac tgcggcaacg agttcgcgta cttctacttt gtctcgttca tcttcctgtg
     6001 ctcgtttctg atgctgaatc tctttgtcgc tgtcatcatg gacaacttcg agtacctcac
     6061 ccgagattct tccatcctgg gcccccatca cttggatgaa tacgtgcgtg tctgggcaga
     6121 gtatgaccct gctgcctgcg gccgcattca ctataaggac atgtacagtt tattgcgagt
     6181 aatatcgccc cctctcggct taggcaagaa atgtcctcat agggttgctt gcaagagact
     6241 cctgcggatg gacctgccgg tagcagatga caacacagtt cacttcaact ccaccctgat
     6301 ggctctgatc cgaaccgccc ttgatatcaa aattgccaag ggtggagctg acaagcagca
     6361 gatggatgca gagcttcgca aggagatgat ggccatatgg cccaacctgt ctcagaagac
     6421 cctggatctg ctggtcacac ctcacaagtc cacggacctg acagtgggta agatctacgc
     6481 agccatgatg atcatggagt attaccggca gagcaaggcc aagaaactgc aggccatgcg
     6541 agaggagcag aaccggacac cactcatgtt ccagcgcatg gagcctccat caccaacaca
     6601 ggagggagga cccagccaga acgcccttcc ctccactcag ctggacccag gaggaggcct
     6661 gatggctcac gaaggcggca tgaaggagag cccgtcctgg gtgacccagc gggcccaaga
     6721 gatgttccag aagactggca cctggagccc agagcgaggg ccacccatcg acatgcctaa
     6781 cagccagccc aactcccagt ctgtggagat gcgagaaatg ggtactgatg gctactcaga
     6841 cagcgaacac tacctcccca tggaaggcca gaccagggcc gcctccatgc cccgcctccc
     6901 agcagagaac cagaggagaa ggggccggcc acgtggaaat gacctcagta ccatctctga
     6961 taccagcccc atgaagcgct cagcctctgt gctgggaccc aaagcccggc gactggatga
     7021 ctactcacta gagcgggtac cgcctgagga gaaccaacgg taccaccaac gtcgccggga
     7081 ccgtggccac cgcacctctg agcgctctct gggccgatac actgatgtgg acacaggttt
     7141 ggggacagat ctgagcatga ccacccaatc gggtgacctg ccctccaaag atcgggacca
     7201 ggaccggggc cgacccaagg accggaagca tcggccacac caccaccacc accaccacca
     7261 tcatcatcat cccccggccc ccgaccggga tcgctacgca caggagcggc cggacaccgg
     7321 ccgggcgcgg gcccgggagc agcgctggtc ccgctcgccc agcgaaggtc gggagcacac
     7381 gacacacaga cagggcagta gttccgtcag tggaagccca gccccctcta cgtccggcac
     7441 cagcacgccg cggcgcggcc gccgccagct cccccagacc ccctgcaccc cgcggccgct
     7501 tgtgtcctac tcgcccgctc cgcgcaggcc tgcggcgcgc aggatggcgg gtcccgcagc
     7561 gccccctggc ggctcgccgc ggggttgccg ccgtgcgccg cgctggcctg cgcatgcgcc
     7621 tgagggcccg cggccgcgcg gtgccgacta caccgaaccc gacagcccgc gcgagccgcc
     7681 agggggcgcg cacgatcccg cgccgcggtc gcccaggact ccgcgcgccg cgggctgcgc
     7741 gtcaccgcgc cacggccgca ggctgcccaa tggttactac gcggggcacg gcgcgccgcg
     7801 gccgcgcaca gcccgcaggg gcgcgcacga cgcgtacagc gagagcgagg acgactggtg
     7861 ctaagccgcc ccgccccccg cagcgcccgt aaagtgcggg cgcactgcaa atgataaaca
     7921 ataacgtttt taaaaaagaa aacccgggag aaaaacaaaa atagcttcta ttgatgagtt
     7981 ttatcatctc gattgaatct ttcgttttcc cggtgaatga aaccgaggtg gctgcaagcc
     8041 ggaaggacgg ctgcacgccc cacacacttc taagccgaat tatccagcac agtggcggcc
     8101 gctcgagtct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     8161 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     8221 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8281 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8341 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8401 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8461 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     8521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9181 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9241 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     9301 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     9361 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     9421 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     9481 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     9541 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     9601 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     9661 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     9721 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
     9781 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     9841 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     9901 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     9961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    10081 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12241 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    12301 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    12361 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    12421 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    12481 ccagatatac gcgtt
//
