LOCUS       sequence-256429-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; 
            in pET21 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     Wagner Lab plasmids
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KE-009
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..168
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKVILNGKTLKGETTTEAVDAATAEKVFEQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             178..195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(814..825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcagtaca aggttatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct
       61 gttgacgctg ctaccgcgga aaaagttttc gaacagtacg ctaacgacaa cggtgttgac
      121 ggtgaatgga cctacgacga cgctaccaaa accttcacgg taaccgaagg tggatctcat
      181 caccatcacc atcacgaaaa cctgtatttt cagggcatgg cggacttcga cacctacgac
      241 gatcgggcct acagcagctt cggcggcggc agagggtccc gcggcagtgc tggtggccat
      301 ggttcccgta gccagaagga gttgcccaca gagcccccct acacagcata cgtaggaaat
      361 ctacctttca atacggttca gggcgacata gatgctatct ttaaggatct cagcataagg
      421 agtgtacggc tagtcagaga caaagacaca gataaattta aaggattctg ctatgtagaa
      481 ttcgatgaag tggattccct taaggaagcc ttgacatacg atggtgcact gttgggcgat
      541 cggtcacttc gtgtggacat tgcagaaggc agaaaacaag ataaaggtgg ctttggattc
      601 agaaaaggtg gaccagatga cagaggcttc agggatgact tcttaggggg caggggaggt
      661 agtcgcccag gcgaccggcg aacaggcccc cccatgggca gccgcttcag agatggccct
      721 cccctccgtg gatccaacat ggatttcaga gaacccacag aagaggaaag agcacagaga
      781 ccacgactcc agcttaaacc tcgaacagtc gcgacgcccc tcaatcaagt agccaatccc
      841 aactctgcta tcttcggggg tgccaggcct agagaggaag tcgttcaaaa ggagcaagaa
      901 tga
//
