LOCUS       sequence-256686-        1788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Mxi1, Part 3b(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM728
FEATURES             Location/Qualifiers
     source          1..1788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..133
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="ERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(153..187)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(258..917)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     832..851
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1099..1687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1588..1607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctgaacgtg tgagaatgat taatgtgcaa aggctgttag aagccgcaga
       61 gtttttagaa agaagagaaa gagaatgcga acacgggtat gccagttctt tccctagcat
      121 gccctctccc agaggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg
      181 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
      241 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
      301 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      361 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      421 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
      481 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
      541 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
      601 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
      661 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
      721 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
      781 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
      841 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
      901 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
      961 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1021 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1081 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1141 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1201 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1261 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1321 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1381 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1441 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1501 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1561 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1621 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1681 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1741 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
