LOCUS       sequence-256687-        5904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Mxi1-dCas9, Part 3(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM729
FEATURES             Location/Qualifiers
     source          1..5904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             11..133
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(4269..4303)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4374..5033)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4948..4967
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5215..5803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5704..5723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggaacgtg tgagaatgat taatgtgcaa aggctgttag aagccgcaga
       61 gtttttagaa agaagagaaa gagaatgcga acacgggtat gccagttctt tccctagcat
      121 gccctctccc agaggcatgg acaagaagta ttctatcgga ctggccatcg ggactaatag
      181 cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt tcaaggtgct
      241 cgggaacacc gaccggcatt ccatcaagaa aaatctgatc ggagctctcc tctttgattc
      301 aggggagacc gctgaagcaa cccgcctcaa gcggactgct agacggcggt acaccaggag
      361 gaagaaccgg atttgttacc ttcaagagat attctccaac gaaatggcaa aggtcgacga
      421 cagcttcttc cataggctgg aagaatcatt cctcgtggaa gaggataaga agcatgaacg
      481 gcatcccatc ttcggtaata tcgtcgacga ggtggcctat cacgagaaat acccaaccat
      541 ctaccatctt cgcaaaaagc tggtggactc aaccgacaag gcagacctcc ggcttatcta
      601 cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg gcgacctcaa
      661 tcctgacaat agcgatgtgg ataaactgtt catccagctg gtgcagactt acaaccagct
      721 ctttgaagag aaccccatca atgcaagcgg agtcgatgcc aaggccattc tgtcagcccg
      781 gctgtcaaag agccgcagac ttgagaatct tatcgctcag ctgccgggtg aaaagaaaaa
      841 tggactgttc gggaacctga ttgctctttc acttgggctg actcccaatt tcaagtctaa
      901 tttcgacctg gcagaggatg ccaagctgca actgtccaag gacacctatg atgacgatct
      961 cgacaacctc ctggcccaga tcggtgacca atacgccgac cttttccttg ctgctaagaa
     1021 tctttctgac gccatcctgc tgtctgacat tctccgcgtg aacactgaaa tcaccaaggc
     1081 ccctctttca gcttcaatga ttaagcggta tgatgagcac caccaggacc tgaccctgct
     1141 taaggcactc gtccggcagc agcttccgga gaagtacaag gaaatcttct ttgaccagtc
     1201 aaagaatgga tacgccggct acatcgacgg aggtgcctcc caagaggaat tttataagtt
     1261 tatcaaacct atccttgaga agatggacgg caccgaagag ctcctcgtga aactgaatcg
     1321 ggaggatctg ctgcggaagc agcgcacttt cgacaatggg agcattcccc accagatcca
     1381 tcttggggag cttcacgcca tccttcggcg ccaagaggac ttctacccct ttcttaagga
     1441 caacagggag aagattgaga aaattctcac tttccgcatc ccctactacg tgggacccct
     1501 cgccagagga aatagccggt ttgcttggat gaccagaaag tcagaagaaa ctatcactcc
     1561 ctggaacttc gaagaggtgg tggacaaggg agccagcgct cagtcattca tcgaacggat
     1621 gactaacttc gataagaacc tccccaatga gaaggtcctg ccgaaacatt ccctgctcta
     1681 cgagtacttt accgtgtaca acgagctgac caaggtgaaa tatgtcaccg aagggatgag
     1741 gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt gtggaccttc tgttcaagac
     1801 caatagaaag gtgaccgtga agcagctgaa ggaggactat ttcaagaaaa ttgaatgctt
     1861 cgactctgtg gagattagcg gggtcgaaga tcggttcaac gcaagcctgg gtacctacca
     1921 tgatctgctt aagatcatca aggacaagga ttttctggac aatgaggaga aagaggacat
     1981 ccttgaggac attgtcctga ctctcactct gttcgaggac cgggaaatga tcgaggagag
     2041 gcttaagacc tacgcccatc tgttcgacga taaagtgatg aagcaactta aacggagaag
     2101 atataccgga tggggacgcc ttagccgcaa actcatcaac ggaatccggg acaaacagag
     2161 cggaaagacc attcttgatt tccttaagag cgacggattc gctaatcgca acttcatgca
     2221 acttatccat gatgattccc tgacctttaa ggaggacatc cagaaggccc aagtgtctgg
     2281 acaaggtgac tcactgcacg agcatatcgc aaatctggct ggttcacccg ctattaagaa
     2341 gggtattctc cagaccgtga aagtcgtgga cgagctggtc aaggtgatgg gtcgccataa
     2401 accagagaac attgtcatcg agatggccag ggaaaaccag actacccaga agggacagaa
     2461 gaacagcagg gagcggatga aaagaattga ggaagggatt aaggagctcg ggtcacagat
     2521 ccttaaagag cacccggtgg aaaacaccca gcttcagaat gagaagctct atctgtacta
     2581 ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt gatatcaaca ggctctcaga
     2641 ctacgacgtg gacgccatcg tccctcagag cttcctcaaa gacgactcaa ttgacaataa
     2701 ggtgctgact cgctcagaca agaaccgggg aaagtcagat aacgtgccct cagaggaagt
     2761 cgtgaaaaag atgaagaact attggcgcca gcttctgaac gcaaagctga tcactcagcg
     2821 gaagttcgac aatctcacta aggctgagag gggcggactg agcgaactgg acaaagcagg
     2881 attcattaaa cggcaacttg tggagactcg gcagattact aaacatgtag cccaaatcct
     2941 tgactcacgc atgaatacca agtacgacga aaacgacaaa cttatccgcg aggtgaaggt
     3001 gattaccctg aagtccaagc tggtcagcga tttcagaaag gactttcaat tctacaaagt
     3061 gcgggagatc aataactatc atcatgctca tgacgcatat ctgaatgccg tggtgggaac
     3121 cgccctgatc aagaagtacc caaagctgga aagcgagttc gtgtacggag actacaaggt
     3181 ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag atcggaaagg ccaccgcaaa
     3241 gtacttcttc tacagcaaca tcatgaattt cttcaagacc gaaatcaccc ttgcaaacgg
     3301 tgagatccgg aagaggccgc tcatcgagac taatggggag actggcgaaa tcgtgtggga
     3361 caagggcaga gatttcgcta ccgtgcgcaa agtgctttct atgcctcaag tgaacatcgt
     3421 gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa tcaatcctcc ccaagcgcaa
     3481 ctccgacaag ctcattgcaa ggaagaagga ttgggaccct aagaagtacg gcggattcga
     3541 ttcaccaact gtggcttatt ctgtcctggt cgtggctaag gtggaaaaag gaaagtctaa
     3601 gaagctcaag agcgtgaagg aactgctggg tatcaccatt atggagcgca gctccttcga
     3661 gaagaaccca attgactttc tcgaagccaa aggttacaag gaagtcaaga aggaccttat
     3721 catcaagctc ccaaagtata gcctgttcga actggagaat gggcggaagc ggatgctcgc
     3781 ctccgctggc gaacttcaga agggtaatga gctggctctc ccctccaagt acgtgaattt
     3841 cctctacctt gcaagccatt acgagaagct gaaggggagc cccgaggaca acgagcaaaa
     3901 gcaactgttt gtggagcagc ataagcatta tctggacgag atcattgagc agatttccga
     3961 gttttctaaa cgcgtcattc tcgctgatgc caacctcgat aaagtcctta gcgcatacaa
     4021 taagcacaga gacaaaccaa ttcgggagca ggctgagaat atcatccacc tgttcaccct
     4081 caccaatctt ggtgcccctg ccgcattcaa gtacttcgac accaccatcg accggaaacg
     4141 ctatacctcc accaaagaag tgctggacgc caccctcatc caccagagca tcaccggact
     4201 ttacgaaact cggattgacc tctcacagct cggaggggat gagggagctg gatcctgaga
     4261 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     4321 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     4381 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     4441 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     4501 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     4561 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     4621 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     4681 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     4741 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     4801 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     4861 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     4921 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     4981 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     5041 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     5101 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     5161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5881 ccctgattct gtggataacc gtag
//
