LOCUS       sequence-256708-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9 NLS, Part 3a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM769
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4124..4144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4179..4213)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4284..4943)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4858..4877
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5125..5713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5614..5633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggacaaga agtattctat cggactggcc atcgggacta atagcgtcgg
       61 gtgggccgtg atcactgacg agtacaaggt gccctctaag aagttcaagg tgctcgggaa
      121 caccgaccgg cattccatca agaaaaatct gatcggagct ctcctctttg attcagggga
      181 aaccgctgaa gcaacccgcc tcaagcggac tgctagacgg cggtacacca ggaggaagaa
      241 ccggatttgt taccttcaag agatattctc caacgaaatg gcaaaggtcg acgacagctt
      301 cttccatagg ctggaagaat cattcctcgt ggaagaggat aagaagcatg aacggcatcc
      361 catcttcggt aatatcgtcg acgaggtggc ctatcacgag aaatacccaa ccatctacca
      421 tcttcgcaaa aagctggtgg actcaaccga caaggcagac ctccggctta tctacctggc
      481 cctggcccac atgatcaagt tcagaggcca cttcctgatc gagggcgacc tcaatcctga
      541 caatagcgat gtggataaac tgttcatcca gctggtgcag acttacaacc agctctttga
      601 agagaacccc atcaatgcaa gcggagtcga tgccaaggcc attctgtcag cccggctgtc
      661 aaagagccgc agacttgaga atcttatcgc tcagctgccg ggtgaaaaga aaaatggact
      721 gttcgggaac ctgattgctc tttcacttgg gctgactccc aatttcaagt ctaatttcga
      781 cctggcagag gatgccaagc tgcaactgtc caaggacacc tatgatgacg atctcgacaa
      841 cctcctggcc cagatcggtg accaatacgc cgaccttttc cttgctgcta agaatctttc
      901 tgacgccatc ctgctgtctg acattctccg cgtgaacact gaaatcacca aggcccctct
      961 ttcagcttca atgattaagc ggtatgatga gcaccaccag gacctgaccc tgcttaaggc
     1021 actcgtccgg cagcagcttc cggagaagta caaggaaatc ttctttgacc agtcaaagaa
     1081 tggatacgcc ggctacatcg acggaggtgc ctcccaagag gaattttata agtttatcaa
     1141 acctatcctt gagaagatgg acggcaccga agagctcctc gtgaaactga atcgggagga
     1201 tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt ccccaccaga tccatcttgg
     1261 ggagcttcac gccatccttc ggcgccaaga ggacttctac ccctttctta aggacaacag
     1321 ggagaagatt gagaaaattc tcactttccg catcccctac tacgtgggac ccctcgccag
     1381 aggaaatagc cggtttgctt ggatgaccag aaagtcagaa gaaactatca ctccctggaa
     1441 cttcgaagag gtggtggaca agggagccag cgctcagtca ttcatcgaac ggatgactaa
     1501 cttcgataag aacctcccca atgagaaggt cctgccgaaa cattccctgc tctacgagta
     1561 ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc
     1621 cgcattcctg tcaggcgaac aaaagaaggc aattgtggac cttctgttca agaccaatag
     1681 aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag aaaattgaat gcttcgactc
     1741 tgtggagatt agcggggtcg aagatcggtt caacgcaagc ctgggtacct accatgatct
     1801 gcttaagatc atcaaggaca aggattttct ggacaatgag gagaaagagg acatccttga
     1861 ggacattgtc ctgactctca ctctgttcga ggaccgggaa atgatcgagg agaggcttaa
     1921 gacctacgcc catctgttcg acgataaagt gatgaagcaa cttaaacgga gaagatatac
     1981 cggatgggga cgccttagcc gcaaactcat caacggaatc cgggacaaac agagcggaaa
     2041 gaccattctt gatttcctta agagcgacgg attcgctaat cgcaacttca tgcaacttat
     2101 ccatgatgat tccctgacct ttaaggagga catccagaag gcccaagtgt ctggacaagg
     2161 tgactcactg cacgagcata tcgcaaatct ggctggttca cccgctatta agaagggtat
     2221 tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga
     2281 gaacattgtc atcgagatgg ccagggaaaa ccagactacc cagaagggac agaagaacag
     2341 cagggagcgg atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac agatccttaa
     2401 agagcacccg gtggaaaaca cccagcttca gaatgagaag ctctatctgt actaccttca
     2461 aaatggacgc gatatgtatg tggaccaaga gcttgatatc aacaggctct cagactacga
     2521 cgtggacgcc atcgtccctc agagcttcct caaagacgac tcaattgaca ataaggtgct
     2581 gactcgctca gacaagaacc ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa
     2641 aaagatgaag aactattggc gccagcttct gaacgcaaag ctgatcactc agcggaagtt
     2701 cgacaatctc actaaggctg agaggggcgg actgagcgaa ctggacaaag caggattcat
     2761 taaacggcaa cttgtggaga ctcggcagat tactaaacat gtagcccaaa tccttgactc
     2821 acgcatgaat accaagtacg acgaaaacga caaacttatc cgcgaggtga aggtgattac
     2881 cctgaagtcc aagctggtca gcgatttcag aaaggacttt caattctaca aagtgcggga
     2941 gatcaataac tatcatcatg ctcatgacgc atatctgaat gccgtggtgg gaaccgccct
     3001 gatcaagaag tacccaaagc tggaaagcga gttcgtgtac ggagactaca aggtctacga
     3061 cgtgcgcaag atgattgcca aatctgagca ggagatcgga aaggccaccg caaagtactt
     3121 cttctacagc aacatcatga atttcttcaa gaccgaaatc acccttgcaa acggtgagat
     3181 ccggaagagg ccgctcatcg agactaatgg ggagactggc gaaatcgtgt gggacaaggg
     3241 cagagatttc gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa
     3301 aaccgaggtg caaaccggag gcttttctaa ggaatcaatc ctccccaagc gcaactccga
     3361 caagctcatt gcaaggaaga aggattggga ccctaagaag tacggcggat tcgattcacc
     3421 aactgtggct tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct
     3481 caagagcgtg aaggaactgc tgggtatcac cattatggag cgcagctcct tcgagaagaa
     3541 cccaattgac tttctcgaag ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa
     3601 gctcccaaag tatagcctgt tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc
     3661 tggcgaactt cagaagggta atgagctggc tctcccctcc aagtacgtga atttcctcta
     3721 ccttgcaagc cattacgaga agctgaaggg gagccccgag gacaacgagc aaaagcaact
     3781 gtttgtggag cagcataagc attatctgga cgagatcatt gagcagattt ccgagttttc
     3841 taaacgcgtc attctcgctg atgccaacct cgataaagtc cttagcgcat acaataagca
     3901 cagagacaaa ccaattcggg agcaggctga gaatatcatc cacctgttca ccctcaccaa
     3961 tcttggtgcc cctgccgcat tcaagtactt cgacaccacc atcgaccgga aacgctatac
     4021 ctccaccaaa gaagtgctgg acgccaccct catccaccag agcatcaccg gactttacga
     4081 aactcggatt gacctctcac agctcggagg ggatgaggga gctcccaaga aaaagcgcaa
     4141 ggtaggtagt tccggatccg gttcttgaga ccagaccaat aaaaaacgcc cggcggcaac
     4201 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     4261 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     4321 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     4381 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4441 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4501 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4561 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4621 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4681 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     4741 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4801 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     4861 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     4921 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     4981 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     5041 ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5461 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5521 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5581 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5641 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5701 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5761 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtag
//
