LOCUS       sequence-256767-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Foight GW, Wang Z, Wei CT, Jr Greisen P, Warner KM, 
            Cunningham-Bryant D, Park K, Brunette TJ, Sheffler W, Baker D, Maly 
            DJ
  TITLE     Multi-input chemical control of protein dimerization for programming
            graded cellular responses.
  JOURNAL   Nat Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8.
  PUBMED    31501561
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 9. pii: 10.1038/s41587-019-0242-8. doi: 
            10.1038/s41587-019-0242-8."
COMMENT     Sequence Label: GWF243
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1440..1643
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1594..1614
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1640..1664
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1726..1735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1735..1764
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(2463..3036,3037..3039,3040..3048)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2630..2647)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2857..2876
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3064..3066,3067..3069,3070..3780)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3064..3066,3067..3069,3070..3780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3109..3130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3370..3389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3717..3738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3793..3822
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5212..5268
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5269..5964
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             5977..6006
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        7557..7768
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             7781..8049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             8076..8675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8076..8095)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8572..8592
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8735..8755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8797..8918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8834..8853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8888..8907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9681..9715
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10206..10222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10206..10222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10219..10241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10230..10246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10254..10260,10261..10278,10279..10284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10299..10320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10437..10454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10608..11196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10688..10707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11367..12158,12159..12227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11990..12009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1441 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1501 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1561 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1621 gggaggttta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     1681 cacgctgttt tgacctccat agaagattct agagctagcg aattcgccac catggagcaa
     1741 aagctgatta gcgaagaaga cctgggtggc aagaagaagg gaagtgtagt catcgtgggt
     1801 aggattaatt tgagtgggga tacggcatac gcacaacaaa ctcgggggga agaaggatgt
     1861 caagagacct ctcaaacggg tagggataag aatcaggtgg aaggagaggt tcaaatcgtt
     1921 tccacggcga cgcaaacatt tcttgcgacc tccattaatg gagtgctttg gacagtatat
     1981 catggagcgg ggaccagaac tatagcctcc ccgaaagggc cagtcacgca aatgtataca
     2041 aacgtggata aagatctcgt agggtggcaa gcacctcaag gctcacgaag tctgactcca
     2101 tgtacgtgtg gatcctccga tctgtatctc gtcacacgcc atgcagatgt aatcccggta
     2161 aggcgaagag gagattcaag aggaagtttg ctgtccccaa gacctatttc ctacctcaag
     2221 ggatcagcag gaggaccctt gctgtgtccc gccgggcacg cagtgggtat attccgcgct
     2281 gctgtcagca caaggggcgt agctaaggcg gtcgatttta ttcctgttga gtcacttgaa
     2341 accacgatgc gatctccatc cgcaggaggg agtgctggag gagagaaaat gtccaaggat
     2401 ggtaagaaaa agaagaagaa gtcaaaaacc aagtgtgtta tcatgtgaat ttaaataatt
     2461 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     2521 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     2581 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     2641 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     2701 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     2761 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     2821 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     2881 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     2941 tgcacatgct ttacgtgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg
     3001 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccgg atccggagct
     3061 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3121 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3181 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3241 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3361 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3481 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3541 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3601 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3661 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3721 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3781 agtggcagtg gcgagcaaaa attgatctca gaggaggatc tgggttccgg gtcttccgac
     3841 gaagaagaag cgcgcgagct gatcgagcgt gcgaaggagg ctgcggaacg cgcgcaagaa
     3901 gctgctgagc gtaccggtga tccgcgcgtg cgtgaactcg ctcgtgagct gaaacgcctg
     3961 gctcaggaag ctgctgaaga agtgaagcgt gacccgtctt cctccgatgt gaacgaagcg
     4021 ctcaaactca tcgtggaagc tatcgaggcg gctgtggacg cgctcgaagc ggctgaacgc
     4081 accggcgacc cggaggttcg tgagctggcg cgtgagctcg tgcgcctcgc ggttgaggct
     4141 gcggaggagg tgcaacgtaa cccatcctcc tctgacgtga atgaggctct ccacagcata
     4201 gtttacgcta ttgaagctgc catttttgca ttggaagctg ccgaaaggac tggtgacccg
     4261 gaagtgagag aactcgcccg agagcttgta agactggcgg ttgaggcggc tgaagaagtc
     4321 cagcgaaatc cgtccagccg gaatgtcgag cacgcactga tgaggattgt gttggccatt
     4381 tacctggcag aagaaaatct ccgcgaagct gaggagtctg gcgatccgga aaaacgtgaa
     4441 aaggctcgcg aacgtgttcg tgaggcggtg gaacgtgcgg aagaggttca gcgtgatcca
     4501 tccggttggc tgaatcatag tgctggtggt agtgcaggag gctcggccgg tggaagtgca
     4561 ggagggagtg gcgctagcag acaactctcg gatgcagata agctgcgcaa ggtgatctgc
     4621 gagctcctgg agacggagcg cacctacgtg aaggatttaa actgtcttat ggagagatac
     4681 ctaaagcctc ttcaaaaaga aacttttctc acccaggatg agcttgacgt gctttttgga
     4741 aatttaacgg aaatggtaga gtttcaagta gaattcctta aaactctaga agatggagtg
     4801 agactggtac ctgatttgga aaagcttgag aaggttgatc aatttaagaa agtgctgttc
     4861 tctctggggg gatcattcct gtattatgct gaccgcttca agctctacag tgccttctgc
     4921 gccagccaca caaaagttcc caaggtcctg gtgaaagcca agacagacac ggctttcaag
     4981 gcattcttgg atgcccagaa cccgaagcag cagcactcat ccacgctgga gtcgtacctc
     5041 atcaagccca tccagaggat cctcaagtac ccacttctgc tcagggagct gttcgccctg
     5101 accgatgcgg agagcgagga gcactaccac ctggacgtgg ccatcaagac catgaacaag
     5161 gttgccagtc acatcaatga gatgcagaaa atccatgaag agggaagcgg agctactaac
     5221 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctag cgagctgatt
     5281 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
     5341 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     5401 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
     5461 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
     5521 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     5581 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     5641 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
     5701 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
     5761 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag
     5821 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
     5881 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
     5941 agcaaactgg ggcacaagct taatagtggc agtggcgagc aaaaattgat ctcagaggag
     6001 gatctgggtt ccgggacggg atctggtact ggttctggta ccgggacaac gtccggaaca
     6061 gggacagggg gctccactgg gatggatatc gagaagcttt gcaaaaaagc tgaagaagag
     6121 gcaaaggagg cgcaagagaa agctgatgag ttgcggcaac ggcaccccga cagccaggct
     6181 gctgaggatg ctgaggactt ggcaaatctg gcggtggccg ctgtcttgac ggcttgcttg
     6241 ttggcccaag aacatccaaa cgctgacatt gcaaagcttt gcattaaggc cgcgtccgaa
     6301 gcggctgaag cagcttctaa ggcagccgag ctggcgcaac ggcatcctga ctcccaagcg
     6361 gcgagggacg ctataaaact cgcatcacaa gcagccagag ctgtaatcct cgctattatg
     6421 ctcgcagccg aaaacccaaa cgctgatatt gccaaactgt gtataaaggc ggctagtgaa
     6481 gccgcagaag ctgcaagcaa agcagcagaa cttgcccaac gccaccccga ctcccaggcg
     6541 gctagagatg ctataaaact tgcttctcag gctgcggagg cggtagaacg agcgatctgg
     6601 ctggcagcag agaaccccaa cgctgacatt gccaaaaagt gtatcaaggc ggcttccgaa
     6661 gccgccgagg aggccagcaa agcggcggaa gaggcacaga gacatccgga ctcacagaaa
     6721 gcacgcgacg agattaaaga ggctagtcaa aaagcagagg aggtaaaaga acgctgtaag
     6781 tcctccgcag gtggcagtgc aggtggcagc gccggcggta gtgccggagg gagtgctggc
     6841 accccaccca actggcagca gcttgttagt cgagaagtgt tactgggact aaaaccttgt
     6901 gaaatcaaaa gacaggaagt gattaatgaa ttgttctaca ctgaaagagc tcatgttcga
     6961 acactgaagg ttcttgatca agtgttctat cagcgagtat ccagagaagg aattctgtca
     7021 ccctcagagc tacggaaaat tttttcaaac ttggaagata ttcttcaact tcatattgga
     7081 ttgaatgaac aaatgaaggc tgttcgaaag agaaatgaga cctctgttat cgatcagatt
     7141 ggggaagatt tgctgacatg gttcagcgga ccaggagagg agaaattgaa acatgctgct
     7201 gctacctttt gcagtaacca acctttcgcc ctggaaatga tcaaatctcg tcagaaaaag
     7261 gattctcgat ttcagacttt tgtgcaagat gctgaaagta atccactgtg tcgtcgtctt
     7321 caactgaagg atattattcc cactcaaatg caaaggctta ctaagtaccc acttctgttg
     7381 gataatattg ccaaatacac agaatggcca acagaaaggg agaaggtgaa gaaagctgca
     7441 gatcactgtc gtcagatctt aaattatgta aatcaggctg tcaaggaggc agaaaacaag
     7501 cagcgctgag gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     7561 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     7621 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     7681 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     7741 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     7801 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     7861 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     7921 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     7981 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     8041 ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc
     8101 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     8161 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     8221 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     8281 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8341 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     8401 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8461 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8521 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8581 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8641 gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca
     8701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8761 acgctgcttt aatgcctttg tatcatgcgt taactaaact tgtttattgc agcttataat
     8821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa ttgactcaaa
     8941 tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac aagacatccc
     9001 tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt gctctggtca
     9061 accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc
     9121 caggtttagc cccggaattg actggattcc ttttttaggg cccattggta tggctttttc
     9181 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     9241 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     9301 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
     9361 agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa
     9421 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     9481 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     9541 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     9601 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     9661 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat
     9721 ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
     9781 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
     9841 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
     9901 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
     9961 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
    10021 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
    10081 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
    10141 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa
    10201 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10261 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10321 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11041 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcat
//
