LOCUS       sequence-257091-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Castaneda AF, Glaunsinger BA
  TITLE     The interaction between ORF18 and ORF30 is required for late gene 
            expression in Kaposi's sarcoma-associated herpesvirus.
  JOURNAL   J Virol. 2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18.
  PUBMED    30305361
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18."
COMMENT     Sequence Label: pLVX-TetOneZeo-ORF18(WT)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    168..302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        350..454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(455..523,524..1315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(673..692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1486..2074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1975..1994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2228..2245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2362..2383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2398..2403,2404..2421,2422..2428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2436..2452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2441..2463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2460..2476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2460..2476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2604..3237
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3444..4032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3550..3561
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3959..3979
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4046..4420)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4429..4758)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4443..4578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(4769..5515)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5534..6044)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5597..5615)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        6061..6428
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6069..6087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6105..6123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6141..6159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6177..6195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6213..6231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6249..6267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6285..6303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             7250..7315
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    7507..7641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7685..7800
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8063..8107)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8292..8525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9022..9147
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9194..9827
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
       61 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
      121 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
      181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      301 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      961 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1081 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1141 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1201 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1261 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2221 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2281 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2461 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaacgcta
     2521 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     2581 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     2641 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     2701 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     2761 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     2821 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     2881 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     2941 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     3001 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     3061 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     3121 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     3181 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
     3241 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     3301 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
     3361 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
     3421 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
     3481 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     3541 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     3601 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     3661 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     3721 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     3781 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     3841 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     3901 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     3961 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     4021 cagaggttga ttgttccaga cgcgttcagt cctgctcctc ggccacgaag tgcacgcagt
     4081 tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca
     4141 tggccggccc ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca
     4201 gctcgtccag gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct
     4261 ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca
     4321 cgaagtcccg ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc
     4381 gcgcggtgag caccggaacg gcactggtca acttggccat ggtcgcgttt tgcaaaagcc
     4441 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     4501 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     4561 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     4621 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     4681 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     4741 acaccctaac tgacacacat tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt
     4801 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     4861 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     4921 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     4981 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     5041 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     5101 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     5161 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     5221 ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     5281 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     5341 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     5401 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     5461 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatggtaa
     5521 ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     5581 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     5641 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     5701 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     5761 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     5821 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     5881 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     5941 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     6001 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc
     6061 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     6121 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     6181 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     6241 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     6301 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     6361 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     6421 ctcgtaaaga attatgctcg gaaaatacgt gtgtgagacc gagccgctga gcccggggct
     6481 taggaggctc atgtggcgct ttttgcaaaa taagaattta aatacattcc acgcccaaga
     6541 gctgcgtttt attcatttgg ttctctgcag gatgtacaat ttcggtctaa atgtgtacct
     6601 gttaagggag gctactgcca atgccgggac ctacgacgag gtggtcctgg gacgcaaggt
     6661 tcctgcggag gtgtggaagc tcgtgtacga tgggctcgag gagatgggcg tgtcaagtga
     6721 gatgctgctg tgtgaggcat accgggacag cctctggatg cacttgaacg ataaggtggg
     6781 gctcttgagg ggcctggcga attatctgtt tcaccggcta ggggtcaccc acgacgttcg
     6841 catcgccccg gaaaacctgg tggacggaaa ctttttgttt aatctgggaa gtgtgctccc
     6901 ctgcaggctg ctccttgcgg cgggctactg cctcgccttt tggggcagcg atgaacacga
     6961 acgctgggtg cgcttcttcg cccagaagct tttcatttgc tacctgatag tctccgggcg
     7021 tcttatgaca cagaggtctc tgctagtttg ggccagcgaa acgggctatc ccggtccggt
     7081 ggaggcagtc tgtcgcgaca tccgctccat gtacggcata cgaacgtatg cggtctcggg
     7141 ttatcttccg gctccgtccg aagcgcagct ggcctacctt ggtgcgttta acaacaacgc
     7201 ggtttcagat ccagcacagt ggcggccgct cgagggaggc ggtggagccg actacaagga
     7261 ccacgacggc gactacaagg accacgacat cgactacaag gacgacgacg acaaggggcc
     7321 cgtttaagat ccagaccacc tcccctgcga gctaagctgg acagccaatg acgggtaaga
     7381 gagtgacatt tttcactaac ctaagacagg agggccgtca gagctactgc ctaatccaaa
     7441 gacgggtaaa agtgataaaa atgtatcact ccaacctaag acaggcgcag cttccgaggg
     7501 atttgagatc cagacatgat aagatacatt gatgagtttg gacaaaccaa aactagaatg
     7561 cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta ttgccttatt tgtaaccatt
     7621 ataagctgca ataaacaagt taagtcgata aactggatct ctgctgtccc tgtaataaac
     7681 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     7741 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     7801 ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac
     7861 cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa
     7921 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta
     7981 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat
     8041 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt
     8101 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat
     8161 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt
     8221 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac
     8281 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct
     8341 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc
     8401 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca
     8461 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag
     8521 ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg
     8581 ctactcctaa tggttcaatt tttactactt tatatttata taattcactt ctccaattgt
     8641 ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt
     8701 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     8761 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     8821 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     8881 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     8941 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     9001 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     9061 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     9121 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     9181 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     9241 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     9301 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     9361 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     9421 ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt
     9481 gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg
     9541 atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat
     9601 cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac
     9661 cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac
     9721 ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca
     9781 cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttcca
//
