LOCUS       sequence-257299-       15294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing AtX and NpgA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 15294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pK_lacO-cP-AOX1-AtX+NpgA
FEATURES             Location/Qualifiers
     source          1..15294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             750..758
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1223..1240
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1320..1566
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     protein_bind    1582..1598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7214..7231
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7311..7557
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(7976..10510)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(10923..11738)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10923..11738)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11646..11665
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11730..11749
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(11903..11922)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    12117..12865
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(12901..12923)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13009..13149
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13064..13083
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13164..13181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13335..13923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13415..13434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14094..14885,14886..14954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14717..14736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14955..15059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15127..15145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    join(15280..15294,1..2)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aatttcacac actaacccct acttgacagc aatatataaa cagaaggaag ctgccctgtc
       61 ttaaaccttt ttttttatca tcattattag cttactttca taattgcgac tggttccaat
      121 tgacaagctt ttgattttaa cgacttttaa cgacaacttg agaagatcaa aaaacaacta
      181 attattcgaa atggtgcaag acacatcaag cgcaagcact tcgccaattt taacaagatg
      241 gtacatcgac acccgccctc taaccgcctc aacagcagcc cttcctctcc ttgaaaccct
      301 ccagcccgct gatcaaatct ccgtccaaaa atactaccat ctgaaggata aacacatgtc
      361 tctcgcctct aatctgctca aatacctctt cgtccaccga aactgtcgca tcccctggtc
      421 ttcaatcgtg atctctcgaa ccccagatcc gcacagacga ccatgctata ttccaccctc
      481 aggctcacag gaagacagct tcaaagacgg atataccggc atcaacgttg agttcaacgt
      541 cagccaccaa gcctcaatgg tcgcgatcgc gggaacagct tttactccca atagtggtgg
      601 ggacagcaaa ctcaaacccg aagtcggaat tgatattacg tgcgtaaacg agcggcaggg
      661 acggaacggg gaagagcgga gcctggaatc gctacgtcaa tatattgata tattctcgga
      721 agtgttttcc actgcagaga tggccaatat aaggaggtta gatggagtct catcatcctc
      781 actgtctgct gatcgtcttg tggactacgg gtacagactc ttctacactt actgggcgct
      841 caaagaggcg tatataaaaa tgactgggga ggccctctta gcaccgtggt tacgggaact
      901 ggaattcagt aatgtcgtcg ccccggccgc tgttgcggag agtggggatt cggctgggga
      961 tttcggggag ccgtatacgg gtgtcaggac gactttatat aaaaatctcg ttgaggatgt
     1021 gaggattgaa gttgctgctc tgggcggtga ttacctattt gcaacggctg cgaggggtgg
     1081 tgggattgga gctagttcta gaccaggagg tggtccagac ggaagtggta tccgaagcca
     1141 ggatccctgg aggcctttca agaagttaga tatagagcga gatatccagc cctgtgcgac
     1201 tggggtgtgt aattgcctat cccatcatca tcatcatcat tgagtttgta gccttagaca
     1261 tgactgttcc tcagttcaag ttgggcactt acgagaagac cggtcttgct agattctaat
     1321 caagaggatg tcagaatgcc atttgcctga gagatgcagg cttcattttt gatacttttt
     1381 tatttgtaac ctatatagta taggattttt tttgtcattt tgtttcttct cgtacgagct
     1441 tgctcctgat cagcctatct cgcagctgat gaatatcttg tggtaggggt ttgggaaaat
     1501 cattcgagtt tgatgttttt cttggtattt cccactcctc ttcagagtac agaagattaa
     1561 gtgagaagat cttgtgtgga attgtgagcg gataacaatt tcacacacta acccctactt
     1621 gacagcaata tataaacaga aggaagctgc cctgtcttaa accttttttt ttatcatcat
     1681 tattagctta ctttcataat tgcgactggt tccaattgac aagcttttga ttttaacgac
     1741 ttttaacgac aacttgagaa gatcaaaaaa caactaatta ttcgaaatgg aggtacatgg
     1801 agatgaagtg ttgtcagtcg actctggcat ctcaactccg ccctcgacag gaggtggatt
     1861 tcggaggcca ctagagactc ccggaacaga aatcgggaat ctcaatctta accctcagaa
     1921 tgaggttgcc gttgttggaa tggcctgccg gcttgccggg ggcaatcatt ctccagaaga
     1981 actgtggcag tccattctaa acaggaagga tgcctctggc gagatcccaa gcatgcgctg
     2041 ggagccgtac taccgtcggg acattcgcaa ccccaagatc ctagatcaaa cgacaaagcg
     2101 cggctacttc ttggaccaca tcgagaattt tgatgccgcg ttctttggcg tttcccccaa
     2161 agaggccgag cagatggacc cccagcagcg gttgtcactc gaggtgactt gggaggccct
     2221 ggaagacgca ggaatcccac cgcagagttt gtccggctca gaaacagccg tgtttatggg
     2281 ggtcaattcg gatgattatt ccaagctctt actggaagat attccgaacg tggaggcctg
     2341 gatgggcatc ggcactgcgt actgcggagt cccgaaccgc atctcctacc acctgaacct
     2401 catggggccc agcactgccg ttgatgccgc ctgtgcctcc tctctcgttg ccatccatca
     2461 cggacgacag gccatcctac aaggcgagag cgaagtcgct attgtcggag gagtcaacgc
     2521 tctctgcggg ccaggactga ctcgcgtact cgataaggca ggagcgacct ccacggaagg
     2581 tcgctgtctc tctttcgacg aagatgcgaa gggctacggc cgtggtgaag gagctgcggt
     2641 ggtgatcttg aaacggctgt ccaccgccat ccgggacgga gaccacattc gcgccatcat
     2701 caagggtagt gccgtagcac aggatggcaa aaccaacggc atcatggctc ccaacgccaa
     2761 ggcacaagag cttgtggcat ggaatgctct tcggacagcc ggagtcgacc ctctgacggt
     2821 tgggtatgtg gaagctcacg caacgtcaac ccctcttggc gatcctaccg aggtcagcgc
     2881 cgtctcagca gtctacggca aaggcagacc ggaagggaat ccttgcttca ttggctctgt
     2941 caaacccaac gtgggccatt tggaagcggg cgctggcgcc gtcggtttca tcaaagcagt
     3001 catggcagtt gaaaaggcca ctttcccccc acaaaccaac ctgaagagac tcaattctcg
     3061 cattgactgg gaccaagccg gagtgaaggt cgtccaggag acactggaat ggcctggcaa
     3121 tgaggatgac gtccgccgag ccggtgtttg ctcttacgga tatggcggta cggtctccca
     3181 tgcaatcatc gaggagtttg cgcaacagct ccaacggccg actaccaaca caaccgatga
     3241 agagcctctg cctcggattc ttctgctgtc ggcacctcaa gagagacgcc ttgctttgca
     3301 ggcacggaca caggcctcct ggattgccgc ggagggcaga aatagaaccc tggagtcgat
     3361 cgcaaccacc ttgagcactc gtcgtgggca ccatgactac cgggctgcca tcatcgcaga
     3421 gaaccatgat gacgctgtgc agaaactgtc tgacattgtc aatggtaaag cagccgaatg
     3481 gacgacgtcg agtcgtgttc tcgatgccag ttgctccaag gacgtggtgt gggttttctc
     3541 cggtcatggc gcacaatgga ctgcaatggc tacggatctc ctcaaagaca ttgtgttcta
     3601 tcaaacaatc agccgtctgg acccgattgt ggagcgcgaa atgggcttct cggcattgca
     3661 ttcccttgca agtggcgatt tcgaatcgtc catcaaggtg caagtgctca cctatctcgt
     3721 acaggtggga ctggctgcca tcttgcgctc gaagggactg gagccccagg ctgtcatcgg
     3781 tcattcagtt ggcgaaattg ccgcctcagt cgcggccggc tgtctgactg cagaagaagg
     3841 cgccctgatt gtcacccgca gagcaaacct ctatcggcgt gtgatgggcg cgggcgcaat
     3901 ggttctcgtc aacattccat tcgccgacat ggagaaagag cttcaaggcc ggacggacct
     3961 ggtggccgcc attgactcct cgccgtcttc atgtgttgtt tccggtgcca ctgaagctgt
     4021 cctggcgctc gtggaagacc tcaagtctcg tggtgtcaac gctttccggg tcaagacgga
     4081 tattcccttc caccacccga tgctggatca gctgtccgag ccattgcgag aggccatgga
     4141 agggtccctg tcgccacgca agcccagagt ccgtctttac tcgacgtcgg cagaagaccc
     4201 acgcagtatg gttgctcggg atatccatta ctggaccagc aacatggtca acccggtccg
     4261 gttgacggcc gcagtgcagg cagcagtgga cgatggcctg cgactgttcc ttgaagtctc
     4321 ttctcatccc attgtgtctc actccgtccg agagaccatg ttggacctgg gtgtggagga
     4381 cttcaccgtg accaacacca tggctcgcaa taagcctgcc gacaagacta ttctgtccag
     4441 cattgcccag cttcactgtc ggggcgccgt cgtcaattgg aagaagcagc tgccgggccc
     4501 ttgggcgctg gatgtgccct tgacgacgtg ggaccacaag cccttctggc ggcatattca
     4561 cactggccct atcagtgcct cgactttgca tgatgtggac aaacacacgc tgttgggtca
     4621 gcgcgttccc gttgcgggag aaacgactat ggtgttcacc acccaaatgg atgaccagac
     4681 caagcctttc ccaggaagcc atccactgca cggctctgag attgttccgg ctgctgccct
     4741 tgtcaacact ttcttgcatg ccaccagggc taccaccctt tccaacatta ctcttcgcgt
     4801 cccagtggcc atcagccagc cgcgcgacat ccaggtggtg gtgtcacaga atcaaatcaa
     4861 gatctgctcc cgtctcactc agaaggcgga ttctggggca gacgaaggtt cctggctgac
     4921 acacactacg ggtcagtggg aagctggtgg aagcaagaac cccccggcgc aactcgatat
     4981 tgctgctatc aaggctcgtc tcgctaataa caagttggcg gacaacttct ccatcgacta
     5041 tttggacaag gttggcgttt cggccatggg cttcccttgg gcagttacag agcactacgg
     5101 caccctgcag gagatgatcg ctcgcgttga tgtcgcgcca gacgtccccg cgaccagtcc
     5161 actcccctgg gatgctgcct cttgggcccc gatcctcgat gcggccacct cagtgggatc
     5221 cacgctcttt ttcgatcagc ctcgcctgcg catgccggct cacattcacg gggttcaagt
     5281 ctacaccacg cagccgcctc ccaaggtggg ttacctgtac gtggaaaagg ctggcgatcg
     5341 ggatctggcg gtgcatgtca gtgtctgcga cgagctcgga accgtcttag ctcgattcga
     5401 atccatgcgc ttttccgaga tcgaaggcac gccgggcagt aacggcagcg aggagagtct
     5461 tgtccaccag ctcgcatggc ctcccgccac ctacagcgag aagccgctga caatcaacaa
     5521 tgtcgtcctc atttcccggg ataggaacgt cgcagatctc tactgtgggt ctttgaaaga
     5581 tcgtgtgtca tctatcacgg tgctggatgc tgctgccgac ctgctttccc tttcgcagga
     5641 tcccagcagt gtcttgcaag caaaggatac agcggtggtg tacgtgcccg gtcccctcca
     5701 cagcgcggat tctatcccga ctgcggccca ttccttcctc atggaattgc tcctcctggt
     5761 caaaatcatt gtcaatggct ctttgcccac caaggtcttt gtccttacgg accgcgtctg
     5821 cgagagtgag tctgcgacgg ctctcgctca gtctccgatc cacggtgtct cccgcatcat
     5881 tgcttcggag cacccagatc aatggggcgg actgattgac gtcgaaacgc cgggccagtt
     5941 cccactcgag acgatgaagt atgtgcagga ggcggacaac atccgcatct cggatggcat
     6001 acccagaatt gctcgtctgc gcccgcttcc tcgcgacaag ctcctaccgc ctagcaagca
     6061 gacttccctg cttccccggc ccgaaggtac ctacttaatt acgggtggac tgggcgctct
     6121 ggggttggag gtcgcacagt tcctggtgga aaagggtgct cgtcgattga tcctcgtttc
     6181 tcggcgtgcc ttgcctccgc gccgggagtg ggcagacatc cttgctgatc catcgtcctc
     6241 gctggcgccg gcgctggaga caatccaggc ccttgagaca cagggagcca ctgtccacac
     6301 cctcgcagtg gacatttcct ctcctgacgc agcgcctcaa ctggcagtcg ccattgatgc
     6361 tctgtcgcta cctccagtcc gcggcgtggt ccacgcagca ggcgttctgg acagccagct
     6421 ggtcctctcc gccacgtcag actctgtcga gcgcgtgctg gcgcccaaga tcaccggagc
     6481 gctggtcctt ggcaccgtct tcccccccaa ggcactcgat ttcttcatgc tattctcctc
     6541 atgcggacag atactaggct tcccaggtca agcatcctac gcgtctggaa acgcgttcct
     6601 tgatgcattc gcaacatcgc gccgacacca aggagacaac gctgtcgccg tgcagtggac
     6661 cagctggcgc tccctcggca tggcagccag taccgacttc atcaacgctg agctagccag
     6721 caagggcatc actgacatca ctcgcgacga ggggttccgc gcgtggatgc atatttccaa
     6781 atatgatatc gaccaggccg ccgtcttgcg cagtctggcc ttcgaggccg atgaacccct
     6841 ccccacccct atccttacgg atattgccgt ccgcaaggct ggctccgcct cctccgctga
     6901 tgctccctct gctgcaccga aagagacgaa cgaaatgccg gaatcgatcc cggagcgtcg
     6961 tacctggttg gatgaacgaa tccgtgattg tgtggcccgc gtgcttcagc tggggagcag
     7021 cgatgaggtt gattccaagg ccgctctgag tgatctggga gtcgacagcg tcatgaccgt
     7081 tagcttgaga ggtcagctgc agaagacgtt gggggtcaag gtgccaccca cactgacctg
     7141 gagttgcccg acggtgtcac atctggtggg atggtttttg gaaaagatgg gaaaggcggc
     7201 cgccagcttt ctacatcatc atcatcatca ttgagtttgt agccttagac atgactgttc
     7261 ctcagttcaa gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat
     7321 gtcagaatgc catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa
     7381 cctatatagt ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga
     7441 tcagcctatc tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt
     7501 ttgatgtttt tcttggtatt tcccactcct cttcagagta cagaagatta agtgagaact
     7561 agtagttcgt ttgtgcaagc ttatcgataa gctttaatgc ggtagtttat cacagttaaa
     7621 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     7681 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     7741 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     7801 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     7861 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     7921 cacacccgtc ctgtggatct atcgaatcta aatgtaagtt aaaatctcta aataattaaa
     7981 taagtcccag tttctccata cgaaccttaa cagcattgcg gtgagcatct agaccttcaa
     8041 cagcagccag atccatcact gcttggccaa tatgtttcag tccctcagga gttacgtctt
     8101 gtgaagtgat gaacttctgg aaggttgcag tgttaactcc gctgtattga cgggcatatc
     8161 cgtacgttgg caaagtgtgg ttggtaccgg aggagtaatc tccacaactc tctggagagt
     8221 aggcaccaac aaacacagat ccagcgtgtt gtacttgatc aacataagaa gaagcattct
     8281 cgatttgcag gatcaagtgt tcaggagcgt actgattgga catttccaaa gcctgctcgt
     8341 aggttgcaac cgatagggtt gtagagtgtg caatacactt gcgtacaatt tcaacccttg
     8401 gcaactgcac agcttggttg tgaacagcat cttcaattct ggcaagctcc ttgtctgtca
     8461 tatcgacagc caacagaatc acctgggaat caataccatg ttcagcttga gacagaaggt
     8521 ctgaggcaac gaaatctgga tcagcgtatt tatcagcaat aactagaact tcagaaggcc
     8581 cagcaggcat gtcaatacta cacagggctg atgtgtcatt ttgaaccatc atcttggcag
     8641 cagtaacgaa ctggtttcct ggaccaaata ttttgtcaca cttaggaaca gtttctgttc
     8701 cgtaagccat agcagctact gcctgggcgc ctcctgctag cacgatacac ttagcaccaa
     8761 ccttgtgggc aacgtagatg acttctgggg taagggtacc atccttctta ggtggagatg
     8821 caaaaacaat ttctttgcaa ccagcaactt tggcaggaac acccagcatc agggaagtgg
     8881 aaggcagaat tgcggttcca ccaggaatat agaggccaac tttctcaata ggtcttgcaa
     8941 aacgagagca gactacacca gggcaagtct caacttgcaa cgtctccgtt agttgagctt
     9001 catggaattt cctgacgtta tctatagaga gatcaatggc tctcttaacg ttatctggca
     9061 attgcataag ttcctctggg aaaggagctt ctaacacagg tgtcttcaaa gcgactccat
     9121 caaacttggc agttagttct aaaagggctt tgtcaccatt ttgacgaaca ttgtcgacaa
     9181 ttggtttgac taattccata atctgttccg ttttctggat aggacgacga agggcatctt
     9241 caatttcttg tgaggaggcc ttagaaacgt caattttgca caattcaata cgaccttcag
     9301 aagggacttc tttaggtttg gattcttctt taggttgttc cttggtgtat cctggcttgg
     9361 catctccttt ccttctagtg acctttaggg acttcatatc caggtttctc tccacctcgt
     9421 ccaacgtcac accgtacttg gcacatctaa ctaatgcaaa ataaaataag tcagcacatt
     9481 cccaggctat atcttccttg gatttagctt ctgcaagttc atcagcttcc tccctaattt
     9541 tagcgttcaa caaaacttcg tcgtcaaata accgtttggt ataagaacct tctggagcat
     9601 tgctcttacg atcccacaag gtggcttcca tggctctaag accctttgat tggccaaaac
     9661 aggaagtgcg ttccaagtga cagaaaccaa cacctgtttg ttcaaccaca aatttcaagc
     9721 agtctccatc acaatccaat tcgataccca gcaacttttg agttgctcca gatgtagcac
     9781 ctttatacca caaaccgtga cgacgagatt ggtagactcc agtttgtgtc cttatagcct
     9841 ccggaataga ctttttggac gagtacacca ggcccaacga gtaattagaa gagtcagcca
     9901 ccaaagtagt gaatagacca tcggggcggt cagtagtcaa agacgccaac aaaatttcac
     9961 tgacagggaa ctttttgaca tcttcagaaa gttcgtattc agtagtcaat tgccgagcat
    10021 caataatggg gattatacca gaagcaacag tggaagtcac atctaccaac tttgcggtct
    10081 cagaaaaagc ataaacagtt ctactaccgc cattagtgaa acttttcaaa tcgcccagtg
    10141 gagaagaaaa aggcacagcg atactagcat tagcgggcaa ggatgcaact ttatcaacca
    10201 gggtcctata gataacccta gcgcctggga tcatcctttg gacaactctt tctgccaaat
    10261 ctaggtccaa aatcacttca ttgataccat tattgtacaa cttgagcaag ttgtcgatca
    10321 gctcctcaaa ttggtcctct gtaacggatg actcaacttg cacattaact tgaagctcag
    10381 tcgattgagt gaacttgatc aggttgtgca gctggtcagc agcataggga aacacggctt
    10441 ttcctaccaa actcaaggaa ttatcaaact ctgcaacact tgcgtatgca ggtagcaagg
    10501 gaaatgtcat acttgaagtc ggacagtgag tgtagtcttg agaaattctg aagccgtatt
    10561 tttattatca gtgagtcagt catcaggaga tcctctacgc cggacgcatc gtggccgacc
    10621 tgcagggggg ggggggcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca
    10681 ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt
    10741 gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt
    10801 gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
    10861 ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct
    10921 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
    10981 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
    11041 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
    11101 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
    11161 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
    11221 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
    11281 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
    11341 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
    11401 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
    11461 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
    11521 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
    11581 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
    11641 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
    11701 ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
    11761 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
    11821 cagagatttt gagacacaac gtggctttcc cccccccccc tgcaggtcgg catcaccggc
    11881 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
    11941 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
    12001 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
    12061 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
    12121 gtatctatga ttggaagtat gggaatggtg atacccgcat tcttcagtgt cttgaggtct
    12181 cctatcagat tatgcccaac taaagcaacc ggaggaggag atttcatggt aaatttctct
    12241 gacttttggt catcagtaga ctcgaactgt gagactatct cggttatgac agcagaaatg
    12301 tccttcttgg agacagtaaa tgaagtccca ccaataaaga aatccttgtt atcaggaaca
    12361 aacttcttgt ttcgaacttt ttcggtgcct tgaactataa aatgtagagt ggatatgtcg
    12421 ggtaggaatg gagcgggcaa atgcttacct tctggacctt caagaggtat gtagggtttg
    12481 tagatactga tgccaacttc agtgacaacg ttgctatttc gttcaaacca ttccgaatcc
    12541 agagaaatca aagttgtttg tctactattg atccaagcca gtgcggtctt gaaactgaca
    12601 atagtgtgct cgtgttttga ggtcatcttt gtatgaataa atctagtctt tgatctaaat
    12661 aatcttgacg agccagacga taataccaat ctaaactctt taaacgttaa aggacaagta
    12721 tgtctgcctg tattaaaccc caaatcagct cgtagtctga tcctcatcaa cttgaggggc
    12781 actatcttgt tttagagaaa tttgcggaga tgcgatatcg agaaaaaggt acgctgattt
    12841 taaacgtgaa atttatctca agatctgctg cctcgcgcgt ttcggtgatg acggtgaaaa
    12901 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    12961 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    13021 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    13081 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    13141 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    13201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    13261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    13321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    13381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    13441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    13501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    13561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    13621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    13681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    13741 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    13801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    13861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    13921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    13981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    14041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    14101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    14161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    14221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    14281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    14341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    14401 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    14461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    14521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    14581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    14641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    14701 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    14761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    14821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    14881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    14941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    15001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    15061 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    15121 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattaattct catgtttgac
    15181 agcttatcat cgataagctg actcatgttg gtattgtgaa atagacgcag atcgggaaca
    15241 ctgaaaaata acagttatta ttcggagctc tgtgtggaat tgtgagcgga taac
//
