LOCUS       sequence-257374-        7672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human Alpha7 (CHRNA7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRNA7
FEATURES             Location/Qualifiers
     source          1..7672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        530..733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     684..704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     778..797
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        778..796
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3009..3026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3015..3239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3285..3713
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3372..3391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3582..3603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3722..3742)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3727..4056
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3907..4042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3969..3988
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4105..5130
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4105..5130
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5260..5381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5297..5316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5351..5370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5430..5446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5430..5446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5443..5465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5454..5470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5478..5484,5485..5502,5503..5508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5523..5544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5661..5678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5832..6420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5912..5931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6591..7382,7383..7451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7214..7233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7452..7556)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7631..7650)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
       61 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      121 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      721 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
      781 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag
      841 ctcggatcca ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg
      901 gcggccgctc gagcggactc aacatgcgct gctcgccggg aggcgtctgg ctggcgctgg
      961 ccgcgtcgct cctgcacgtg tccctgcaag gcgagttcca gaggaagctt tacaaggagc
     1021 tggtcaagaa ctacaatccc ctggagaggc ccgtggccaa tgactcgcaa ccactcaccg
     1081 tctacttctc cctgagcctc ctgcagatca tggacgtgga tgagaagaac caagttttaa
     1141 ccaccaacat ttggctgcaa atgtcttgga cagatcacta tttacagtgg aatgtgtcag
     1201 aatatccagg ggtgaagact gttcgtttcc cagatggcca gatttggaaa ccagacattc
     1261 ttctctataa cagtgctgat gagcgctttg acgccacatt ccacactaac gtgttggtga
     1321 attcttctgg gcattgccag tacctgcctc caggcatatt caagagttcc tgctacatcg
     1381 atgtacgctg gtttcccttt gatgtgcagc actgcaaact gaagtttggg tcctggtctt
     1441 acggaggctg gtccttggat ctgcagatgc aggaggcaga tatcagtggc tatatcccca
     1501 atggagaatg ggacctagtg ggaatccccg gcaagaggag tgaaaggttc tatgagtgct
     1561 gcaaagagcc ctaccctgat gtcaccttca cagtgaccat gcgccgcagg acactctact
     1621 atggcctcaa cctgctgatc ccctgtgtgc tcatctccgc cctcgccctg ctggtgttcc
     1681 tgcttcctgc agattccggg gagaagattt ccctggggat aacagtctta ctctctctta
     1741 ccgtcttcat gctgctcgtg gctgagatca tgcccgcaac atccgattcg gtaccattga
     1801 tagcccagta cttcgccagc accatgatca tcgtgggcct ctcggtggtg gtgacggtga
     1861 tcgtgctgca gtaccaccac cacgaccccg acgggggcaa gatgcccaag tggaccagag
     1921 tcatccttct gaactggtgc gcgtggttcc tgcgaatgaa gaggcccggg gaggacaagg
     1981 tgcgcccggc ctgccagcac aagcagcggc gctgcagcct ggccagtgtg gagatgagcg
     2041 ccgtggcgcc gccgcccgcc agcaacggga acctgctgta catcggcttc cgcggcctgg
     2101 acggcgtgca ctgtgtcccg acccccgact ctggggtagt gtgtggccgc atggcctgct
     2161 cccccacgca cgatgagcac ctcctgcacg gtgggcaacc ccccgagggg gacccggact
     2221 tggccaagat cctggaggag gtccgctaca ttgccaaccg cttccgctgc caggacgaaa
     2281 gcgaggcggt ctgcagcgag tggaagttcg ccgcctgtgt ggtggaccgc ctgtgcctca
     2341 tggccttctc ggtcttcacc atcatctgca ccatcggcat cctgatgtcg gctcccaact
     2401 tcgtggaggc cgtgtccaaa gactttgcgt aaccacgcct ggttctgtac atgtggaaaa
     2461 ctcacagatg ggcaaggcct ttggcttggc gagatttggg ggtgctaatc caggacagca
     2521 ttacacgcca caactccagt gttcccttct ggctgtcagt cgtgttgctt acggtttctt
     2581 tgttacttta ggtagtagaa tctcagcact ttgtttcata ttctcagatg ggctgataga
     2641 tatccttggc acatccgtac catcggtcag cagggccact gagtagtcat tttgcccatt
     2701 agcccactgc ctggaaagcc cttcggagag ctccccatgg ctcctcacca ccgagacagt
     2761 tggttttgca tgtctgcatg aaggtctacc tgaaaattca acatttgctt tttgcttgtg
     2821 tacaaaccca gattgaagct aaaataaacc agactcacta aatcctttcc aaaaaaaaaa
     2881 aaaaaaaaaa aaanccggaa ttcctgcagc ccgggggatc cactagtcca gtgtggtgga
     2941 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg
     3001 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3061 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3121 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3181 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3241 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     3301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3361 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3481 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3601 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3661 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     3721 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3781 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3841 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3901 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3961 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4021 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     4081 attttcggat ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     4141 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     4201 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     4261 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     4321 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     4381 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     4441 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     4501 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     4561 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     4621 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     4681 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     4741 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     4801 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     4861 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     4921 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     4981 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     5041 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     5101 cccagcactc gtccgagggc aaaggaatag cacgtgctac gagatttcga ttccaccgcc
     5161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5221 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     5281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     5401 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5581 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5641 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7561 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     7621 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ct
//
