LOCUS       sequence-257507-        2617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xHA-HPS1 construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Martina JA, Moriyama K, Bonifacino JS
  TITLE     BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome 
            gene products HPS1 and HPS4.
  JOURNAL   J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 
            10.1074/jbc.M301294200. Epub 2003 May 19.
  PUBMED    12756248
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M301294200"; journalName: "J Biol Chem"; date: 
            "2003-08-1- 1"; volume: "278"; issue: "31"; pages: "29376-84"
COMMENT     Sequence Label: pCI-HA3-HPS1
FEATURES             Location/Qualifiers
     source          1..2617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          149..281
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     264..283
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        326..344
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             366..392
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             396..422
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             429..455
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgtcagatca ctagaagctt tattgcggta gtttatcaca gtnaanttgc taacgcagtc
       61 agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa ggtagccttg
      121 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
      181 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      241 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
      301 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc tcgagcgcca
      361 ccatgtaccc gtacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
      421 ccatgggcta cccttacgac gttccagatt acgctaagct tatgaagtgc gtcttggtgg
      481 ccactgaggg cgcagaggtc ctcttctact ggacagatca ggagtttgaa gagagtctcc
      541 ggctgaagtt cgggcagtca gagaatgagg aagaagagct ccctgccctg gaggaccagc
      601 tcagcaccct cctagccccg gtcatcatct cctccatgac gatgctggag aagctctcgg
      661 acacctacac ctgcttctcc acggaaaatg gcaacttcct gtatgtcctt cacctgtttg
      721 gagaatgcct gttcattgcc atcaatggtg accacaccga gagcgagggg gacctgcggc
      781 ggaagctgta tgtgctcaag tacctgtttg aagtgcactt tgggctggtg actgtggacg
      841 gtcatcttat ccgaaaggag ctgcggcccc cagacctggc gcagcgtgtc cagctgtggg
      901 agcacttcca gagcctgctg tggacctaca gccgcctgcg ggagcaggag cagtgcttcg
      961 ccgtggaggc cctggagcga ctgattcacc cccagctctg tgagctgtgc atagaggcgc
     1021 tggagcggca cgtcatccag gctgtcaaca ccagccccga gcggggaggc gaggaggccc
     1081 tgcatgcctt cctgctcgtg cactccaagc tgctggcatt ctactctagc cacagtgcca
     1141 gctccctgcg cccggccgac ctgcttgccc tcatcctcct ggttcaggac ctctacccca
     1201 gcgagagcac agcagaggac gacattcagc cttccccgcg gagggcccgg agcagccaga
     1261 acatccccgt gcagcaggcc tggagccctc actccacggg cccaactggg gggagctctg
     1321 cagagacgga gacagacagc ttctccctcc ctgaggagta cttcacacca gctccttccc
     1381 ctggcgatca gagctcaggt agcaccatct ggctggaggg gggcaccccc cccatggatg
     1441 cccttcagat agcagaggac accctccaaa cactggttcc ccactgccct gtgccttccg
     1501 gccccagaag gatcttcctg gatgccaacg tgaaggaaag ctactgcccc ctagtgcccc
     1561 acaccatgta ctgcctgccc ctgtggcagg gcatcaacct ggtgctcctg accaggagcc
     1621 ccagcgcgcc cctggccctg gttctgtccc agctgatgga tggcttctcc atgctggaga
     1681 agaagctgaa ggaagggccg gagcccgggg cctccctgcg ctcccagccc ctcgtgggag
     1741 acctgcgcca gaggatggac aagtttgtca agaatcgagg ggcacaggag attcagagca
     1801 cctggctgga gtttaaggcc aaggctttct ccaaaagtga gcccggatcc tcctgggagc
     1861 tgctccaggc atgtgggaag ctgaagcggc agctctgcgc catctaccgg ctgaactttc
     1921 tgaccacagc ccccagcagg ggaggcccac acctgcccca gcacctgcag gaccaagtgc
     1981 agaggctcat gcgggagaag ctgacggact ggaaggactt cttgctggtg aagagcagga
     2041 ggaacatcac catggtgtcc tacctagaag acttcccagg cttggtgcac ttcatctatg
     2101 tggaccgcac cactgggcag atggtggcgc cttccctcaa ctgcagtcaa aagacctcgt
     2161 cggagttggg caaggggccg ctggctgcct ttgtcaaaac taaggtctgg tctctgatcc
     2221 agctggcgcg cagatacctg cagaagggct acaccacgct gctgttccag gagggggatt
     2281 tctactgctc ctacttcctg tggttcgaga atgacatggg gtacaaactc cagatgatcg
     2341 aggtgcccgt cctctccgac gactcagtgc ctatcggcat gctgggagga gactactaca
     2401 ggaagctcct gcgctactac agcaagaacc gcccaaccga ggctgtcagg tgctacgagc
     2461 tgctggccct gcacctgtct gtcatcccca ctgacctgct ggtgcagcag gccggccagc
     2521 tggcccggcg cctctgggag gcctcccgta tccccctgct ctaggaattc acgcgtggta
     2581 cctctagagt cgacccgggc ggccgcttcc ctttagt
//
