LOCUS       sequence-257516-        2654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xMyc-HPS4 construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Martina JA, Moriyama K, Bonifacino JS
  TITLE     BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome 
            gene products HPS1 and HPS4.
  JOURNAL   J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 
            10.1074/jbc.M301294200. Epub 2003 May 19.
  PUBMED    12756248
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M301294200"; journalName: "J Biol Chem"; date: 
            "2003-08-1- 1"; volume: "278"; issue: "31"; pages: "29376-84"
COMMENT     Sequence Label: pCI-Myc3-HPS4
FEATURES             Location/Qualifiers
     source          1..2654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          140..272
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     255..274
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        317..335
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      347..356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             356..385
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             389..418
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             425..454
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actagaagct ttattgcggt agtttatcac agttaanttg ctaacgcagt cagtgcttct
       61 gacacaacag tctcgaactt aagctgcagt gactctctta aggtagcctt gcagaagttg
      121 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      181 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      241 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
      301 ttaaggctag agtacttaat acgactcact ataggctagc ctcgaggcca ccatggagca
      361 gaagctgatc tccgaagagg acctcggcga acagaaactc atctctgagg aagatctgat
      421 gggggaacag aagctgattt ccgaggaaga cctggaattc atggccacct ctacctccac
      481 agaggcaaag tcagcctcgt ggtggaatta tttttttctt tatgatggtt ccaaggtaaa
      541 ggaagaaggc gatccaacaa gagctggcat ttgttacttt tatccttccc agaccctgct
      601 agaccaacag gagttgcttt gtggacagat tgctggagtt gtccgctgtg tttctgacat
      661 ttctgactct cctcctactc ttgttcgtct gagaaaactg aagtttgcca taaaagttga
      721 tggagattac ctttgggtgc tgggctgtgc tgtggagctc cctgatgtca gctgcaagcg
      781 gtttctggat cagctagttg gattctttaa tttttacaat ggacctgttt ccctagctta
      841 tgagaactgt tctcaggaag aactgagcac ggagtgggac accttcatcg agcaaattct
      901 gaaaaacacc agtgatctgc ataagatttt caattccctc tggaacttgg accaaactaa
      961 agtggagccc ctgttgttgc tgaaggcagc ccgcattctg cagacctgcc agcgctcgcc
     1021 tcacattctc gctggctgca tcctctataa aggactgatt gtcagcaccc aactcccgcc
     1081 ctccctcacc gccaaggtcc tgcttcaccg aacagcacct caggagcaga gactccctac
     1141 gggaggggat gccccgcagg aacatggagc ggcattgccc ccgaatgtcc agattatccc
     1201 tgtttttgtg accaaagagg aagccattag tctccacgag ttcccggtgg aacagatgac
     1261 aaggtctcta gcatctccag caggactcca ggatggttca gcccagcacc atccaaaggg
     1321 tgggagcaca tctgccctga aagaaaacgc cactggccat gtggaatcca tggcctggac
     1381 caccccagat cccacatccc ctgacgaagc ttgtccagat ggcaggaagg agaacggatg
     1441 cttgtctggc catgatctgg agagcatcag gcccgcagga ctgcacaact ctgccagggg
     1501 tgaggttctt ggcctcagct cctccctggg gaaggaacta gtctttctcc aagaagaact
     1561 cgacttgtct gaaatccaca ttccagaggc tcaggaagtg gaaatggcct caggtcattt
     1621 tgccttccta catgtgcctg ttccagatgg cagggctcct tactgcaagg catctctcag
     1681 cgcctccagc agcctggaac ccacgcctcc tgaggacaca gccatcagca gcttgcgccc
     1741 tccctctgct cctgagatgc tgacccagca tggagcccaa gagcaggtcg aagaccatcc
     1801 tggccatagc agccaagccc ccattcccag agcagaccct ctccccagaa ggacccgcag
     1861 gcccttgtta ttgcctcgct tagatccagg acagagagga aacaagcttc ccacggggga
     1921 acaaggcctg gatgaggatg ttgatggggt ctgtgaaagc cacgcagccc ctggtctgga
     1981 atgcagttca ggctcagcaa actgtcaggg tgctggcccc tctgcagatg gaatcagctc
     2041 caggctgaca ccagcagagt cctgcatggg gctcgtgagg atgaatctct acactcactg
     2101 cgtcaaaggg ctgatgctgt ccctgctggc tgaggagccg ctgctgggag acagcgcagc
     2161 catagaggaa gtgtaccaca gcagcctggc ttcactgaat gggctggaag tccacctgaa
     2221 agagacgctg cccagggatg aggcagcctc cacgagcagc acctacaact tcacatatta
     2281 cgaccgcatt cagagcttgc tgatggcaaa cctgccgcag gtggccaccc cgcatgatcg
     2341 ccgcttcctc caggccgtca gcctgatgca tagcgaattt gcccagctgc ccgcgcttta
     2401 tgaaatgact gtcagaaatg cctccacggc tgtgtacgcc tgttgcaacc ccatccagga
     2461 gacatatttc cagcagctgg cacctgcagc acggagctcc ggcttcccaa accctcagga
     2521 tggcgccttc agcctctccg gcaaagcaaa gcagaagctg ctgaagcacg gggtgaactt
     2581 gctctgaccg ggggaagggc gaattcacgc gtggtacctc tagagtcgac ccgggcggcc
     2641 gcttcccttt agtg
//
