LOCUS       sequence-257571-       13636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multiplex luciferase reporter vector, with luciferase reporters for 
            the pathways NFKB, SMAD, FOXO, P53 and AP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: MLRV2: NF-kb-SMAD-DBE-P53-AP1
FEATURES             Location/Qualifiers
     source          1..13636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(1955..2158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1984..2004)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2159..2462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    2540..2588
                     /note="synthetic polyadenylation signal"
     misc_feature    2602..2693
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        2806..2837
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    4522..4746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4759..4807
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        5073..5104
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             5138..6790
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5204..5222)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6798..7022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7035..7083
                     /note="synthetic polyadenylation signal"
     misc_feature    7097..7188
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        7336..7367
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    8341..8565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8578..8626
                     /note="synthetic polyadenylation signal"
     misc_feature    8640..8731
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        8844..8875
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    9429..9653
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    9666..9714
                     /note="synthetic polyadenylation signal"
     misc_feature    9728..9819
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        9942..9973
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    10947..11171
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(11279..11867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11359..11378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12335..13126
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      13181..13609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13268..13287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13478..13499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
       61 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
      121 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
      181 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagaa
      241 gctcaagccg ccagggcata gttctcatcg ttggcggcgg atgtcttgta catagatcca
      301 ccacccgcct tctccataag tgcttctcgg agttcctttc ggctgatttt gcccgtgacg
      361 ttcctcggaa tagagtcaac gaatctgaca ccccctcgaa gatacttgct gtggctaact
      421 ctttgtgcaa ggaaatcata tacttcttta gccgtcagtt gggccccagg ttgcttgaca
      481 acgaatccgg ccggaagttc ccctgcttct atgtcgggga taccgactac ggccacatcg
      541 cggatccctg gatgttggag caaaatctcc tccaactcga caggagccac ttgatatccc
      601 ttgtacttga tcagctcttt atatctatcc acaatgtaaa aatattcatc ctcatcatag
      661 tagccaaaat caccactgtg aagccaacca tcgtcgtcaa tggcctcctt ggtggcctgt
      721 ggattattca cgtagccttt cgtgaccatg gggccccaaa tgcacaactc tcccacttga
      781 tttgggccaa gcgcctctcc ggtgtttcga tctataattt tagcggccat gtagggcgtc
      841 acttttccca atgaaccgga tttaaattcg ttgtgaagag tgtggatatt agcggatgtg
      901 ctttcagtca acccgtaacc acaccgtatg cctggcaaat taaggcgttt tacggcgatt
      961 tcagctactt cttttgcaag aggtgccgca ccacaacaaa gttccgccaa ggtagaaaga
     1021 tcatacttat caaccaacgg tgatttagaa agaaagagga ttgtacttgg gacattgatc
     1081 acacttcgta cttcatagtc ttgaatcgcc ttcaaaaaaa cctcttggtt gaacctccga
     1141 agcatcacga cgcgcagccc caccataaaa taccccaagt taatggaaaa cccaaaggcg
     1201 tgaaagaagg gaagatacgc cagcacacta actccgggaa tgagttgggt accaactctc
     1261 gggtcagagg cgtgggtcag ccgaacacat atattgcggt gggtctgcat gacgccctta
     1321 ggcaacccgg tagtaccact actacacagt atcgctgcaa cctggtcgac cggatcgtaa
     1381 tgcagtggtt taaaggtctg aagattgtta tcgctatatc ttgacataaa attatgcaag
     1441 gattcacatc cgagaaggtt ttcctcagag tccagaatta tgattctttt gatataattt
     1501 gttctatcct taacctccaa aacctttgga agtatctttc gcgtggtgaa cacaagtatt
     1561 ggcttactaa ttcctgtgac cttacacagt tcgtctggta tatagtcctc gtttacaggt
     1621 gcaacgacca tgcctatata ccacgcacta ataattggaa tgaaaaatct tttgttgttt
     1681 tcagcgcata ttgatacaac atcattcatt ttatagccac aattctggag actctgcccc
     1741 aatttgaccg tcgtgtcgaa gaattcctga taactaagac tttcgttacc catcacgtcg
     1801 aggatcgcct gcgggatgtg actatgtttg tgaagcgcgt tgtagagcat ctctccagcg
     1861 gtaaaattcc caagcgggtg cttaggctcg ggcccataca caacattctt ctctctcttc
     1921 atcatttgag cttgatctga cggttcacta aaccagctct gcttatatag acctcccacc
     1981 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2041 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2101 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     2161 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2341 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     2461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     2521 tactccggat cccagggaga ataaaatatc tttattttca ttacatctgt gtgttggttt
     2581 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     2641 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctcgcatgc
     2701 atgaggggaa tttccgggga ctttcccggg aatttgcggg ggactttccg ggaatttcct
     2761 ccctcgagga tatcaaatct ggcctcggcg gccaagctta gacactagag ggtatataat
     2821 ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca atggagaaca
     2881 tggagaacga cgagaacatt gtggtgggac ccaagccgtt ctacccgatc gaggagggtt
     2941 ccgctggcac ccagctgcgc aagtacatgg agcgctacgc taagctgggt gctatcgcct
     3001 tcaccaacgc cgtgaccggt gtggattact cctacgccga gtacctggag aagtcctgct
     3061 gcctcggaaa ggccctgcag aactacggtc tggtcgtgga tggccgcatt gccctctgct
     3121 cggagaactg cgaggagttc ttcattcccg tgatcgctgg tctgttcatc ggagtcggcg
     3181 ttgccccaac caacgagatc tacaccctgc gcgagctggt ccacagcctg ggtatcagca
     3241 agccgaccat cgttttctcg agcaagaagg gcctcgacaa ggttatcacc gtgcaaaaaa
     3301 ccgtcaccac catcaagacc atcgttatcc tggattccaa ggttgactac cgcggttacc
     3361 agtgcctcga caccttcatc aagcgtaaca ccccacccgg cttccaagcc tcctccttca
     3421 aaaccgtgga ggttgatcgc aaggagcagg tggccctgat catgaactcc tccggctcca
     3481 ccggtctgcc aaagggcgtg cagctgaccc acgagaacac cgtcacccgc ttctcgcatg
     3541 cccgtgatcc catctacggc aaccaggtgt ccccaggcac cgccgtcctg accgtcgtgc
     3601 ccctccacca cggcttcggc atgttcacca ctctcggcta cttgatctgc ggcttccgtg
     3661 tggtgatgct gactaagttc gacgaggaga ctttcctcaa gaccctgcaa gactacaagt
     3721 gcacttacgt gatcctggtg ccgaccctgt tcgccatcct gaacaagagc gagctgttga
     3781 acaaatacga cctgtcgaac ctggtggaga tcgcttccgg cggcgcccca ctgtcgaagg
     3841 aagttggcga ggccgttgct cgccgcttca acctgcccgg cgtccgtcag ggttacggcc
     3901 tgaccgagac tacctcggct atcatcatca cccccgaggg cgacgataag cccggcgctt
     3961 ccggcaaggt tgttccactc ttcaaagcca aggtgatcga cctggacacc aagaagtccc
     4021 tgggtcccaa ccgtcgcgga gaagtgtgcg tcaagggccc catgctgatg aagggctacg
     4081 tcaacaaccc agaggccacc aaggagttga tcgacgagga gggctggctg cacactggtg
     4141 acatcggtta ctacgacgaa gagaagcact tcttcatcgt tgaccgcctg aagtccttga
     4201 tcaagtacaa gggctaccag gttccccccg ccgagctcga gagcgtcctg ctgcagcacc
     4261 cctccatttt cgatgccgga gtggccggtg tccccgatcc cgtggctggc gagctgccag
     4321 gtgccgtcgt cgtgctggag tccggcaaga acatgaccga gaaggaagtg atggattacg
     4381 tggcctcgca agtgtccaac gccaagcgcc tgcgcggcgg cgtgcgcttc gtggatgagg
     4441 tgcccaaggg cctcaccgga aagatcgacg gtcgcgctat ccgcgagatc ctcaagaagc
     4501 ccgtggccaa gatgtgagct tctgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4741 ctatggcgct gtcaggagaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4801 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4861 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctcgctgtca
     4921 ggaggtctag actgccgtct agacttaaca gtacgtctag actcggccgt ctagacttag
     4981 tacgtctaga ctcggtatgt ctagactgac ccgtctagac ggatcctccc tcgaggatat
     5041 caaatctggc ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt
     5101 ccagcttggc aatccggtac tgttggtaaa gccaccaatg gaagacgcca aaaacataaa
     5161 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     5221 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     5281 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     5341 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     5401 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     5461 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     5521 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     5581 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     5641 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     5701 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     5761 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     5821 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     5881 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     5941 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     6001 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     6061 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     6121 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     6181 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     6241 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     6301 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     6361 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     6421 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     6481 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     6541 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     6601 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     6661 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     6721 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     6781 cgccgtgtaa tgagcttctg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7021 ggcgctaatg ggagaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     7081 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     7141 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg ctgaattcga
     7201 tcaagtaaac aactatgtaa acaagatgca gatcaagtaa acaactatgt aaacaactcc
     7261 gttgatcaag taaacaacta tgtaaacaat ccctcgagga tatcaaatct ggcctcggcg
     7321 gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg
     7381 tactgttggt aaagccacca atgacttcca aggtttatga ccccgagcaa cgcaagagaa
     7441 tgataactgg gcctcagtgg tgggccagat gtaaacaaat gaatgtcctt gatagtttca
     7501 tcaattacta cgacagtgaa aagcatgcag aaaacgccgt aatttttttg catggcaacg
     7561 cagcctcctc ttatttgtgg cgacacgtag tcccgcatat agagccggta gctaggtgta
     7621 ttatccctga tctgatcgga atgggaaaga gtggcaagtc agggaatgga tcttatcgcc
     7681 ttcttgacca ctacaagtat ctgacggctt ggttcgaact cttgaatttg ccgaagaaaa
     7741 tcatcttcgt gggtcacgat tggggagcct gtctcgcgtt ccattattcc tacgagcatc
     7801 aggacaagat aaaggcaatc gttcacgccg agtctgtagt agatgtaatc gagtcctggg
     7861 acgaatggcc agatattgag gaggatatag cattgattaa aagcgaggaa ggggaaaaga
     7921 tggtgctgga gaacaacttc tttgtggaaa caatgcttcc ttcaaaaatc atgagaaaac
     7981 tggaacccga ggaatttgcc gcttatctcg aaccgtttaa agaaaaggga gaagtccgca
     8041 ggccaacact ttcctggcca agagaaatcc cgctcgtcaa aggcggaaag ccggatgttg
     8101 tgcaaatagt ccgaaattac aacgcgtacc tccgggcctc tgacgatctc cctaaaatgt
     8161 ttatagaatc agatccagga ttttttagca acgctatcgt agaaggagca aagaaatttc
     8221 caaataccga atttgtcaaa gtaaagggac tgcatttcag tcaagaggac gctccagacg
     8281 agatgggcaa atacataaag tcctttgtag agagggtcct gaagaacgaa cagtgagctt
     8341 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8401 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8461 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8521 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcgctg cttggagaat
     8581 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     8641 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     8701 agtgcaagtg caggtgccag aacatttctc tcgctgtcag gagtacagaa catgtctaag
     8761 catgctgtgc cttgcctgga cttgcctggc cttgccttcc ctcgaggata tcaaatctgg
     8821 cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg
     8881 caatccggta ctgttggtaa agccaccaat ggtgtttacc ttggaggact tcgtgggtga
     8941 ctggaggcaa actgcaggat acaatttgga ccaggtcctc gaacagggag gagtcagcag
     9001 ccttttccaa aacttgggcg tgtccgttac ccctattcag agaattgtcc tttccggaga
     9061 gaacggattg aaaatagata tccacgtcat aatcccgtat gaaggactgt ctggggacca
     9121 aatgggtcag attgaaaaaa tattcaaagt agtttatccc gttgatgatc atcacttcaa
     9181 agttatcctt cattatggga ctttggtaat cgatggagtt actcctaata tgatagatta
     9241 ctttggccga ccttacgagg ggatcgctgt attcgacggc aagaaaatca ccgtcactgg
     9301 aaccctctgg aacggcaaca agattattga cgagcgattg atcaatccag acggatcttt
     9361 gcttttccgc gtaacgataa acggcgtaac agggtggagg ctctgcgaac gaatactcgc
     9421 ctgagcttct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9481 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9541 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9601 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcgcttgg
     9661 cggagaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     9721 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     9781 ctgtccccag tgcaagtgca ggtgccagaa catttctctc gctgcagact atgagtcaaa
     9841 cagcgtgact caagttgagt cagttgactc acttgagtca ctgactcagg catgctccct
     9901 cgaggatatc aaatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa
     9961 gctcgacttc cagcttggca atccggtact gttggtaaag ccaccaatgg cttccaaagt
    10021 ctacgaccca gaacaaagga agaggatgat tactggaccg cagtggtggg caaggtgcaa
    10081 gcagatgaat gtactcgata gttttatcaa ttattacgac tccgaaaaac acgcggaaaa
    10141 tgctgtcata ttcctgcacg ggaacgccac tagcagctac ttgtggcggc acgtagtccc
    10201 ccacatagag cctgttgccc gatgtattat tcccgatctc attggaatgg gcaaatcagg
    10261 caaatctggg aacggttctt accgcctgct cgatcactat aaatatttga ccgcttggtt
    10321 tgagctgctt aatctcccta aaaaaataat attcgttgga cacgactggg gctcagctct
    10381 ggcgttccac tacgcatacg agcatcaaga ccgaataaaa gcaatagtac atatggagtc
    10441 agttgttgac gtcatagagt catggatggg ttggccagat attgaagagg aattggccct
    10501 catcaagagt gaggaggggg aaaaaatggt cctggaaaat aattttttcg tggaaacctt
    10561 gctcccgagt aaaataatgc gaaaacttga acccgaagag tttgctgctt atttggagcc
    10621 tttcaaggaa aagggggaag taagacgacc aaccctttct tggccgaggg agatacctct
    10681 tgttaagggt ggaaagcccg acgtagtcca gatagtccga aattacaacg cgtacctgag
    10741 agctagtgat gacctcccta agttgtttat cgagtcagac cctgggttct tcagcaacgc
    10801 aattgtagaa ggagcgaaaa aatttccaaa tacagaattt gtgaaggtta aggggctcca
    10861 ctttttgcaa gaggacgccc ccgatgagat gggaaaatac attaaatctt tcgtagagcg
    10921 cgtccttaag aatgaacagt gagcttctgt gccttctagt tgccagccat ctgttgtttg
    10981 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    11041 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    11101 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    11161 gggctctatg gcgcttgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11941 tgagattatc aaaaaggatc ttcacctaga tccttttgtg accagccagg acagaaatgc
    12001 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
    12061 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
    12121 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
    12181 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
    12241 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
    12301 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
    12361 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
    12421 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
    12481 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
    12541 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
    12601 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
    12661 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
    12721 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
    12781 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
    12841 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
    12901 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtagctgc
    12961 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
    13021 ggcttatctt ggacaagaag aagatcgttt ggcctcgcgc gcagatcagt tggaagaatt
    13081 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatcac tagaccaatg
    13141 ttacacatat atactttaga ttgatttaaa acttcatgtt acgcgccctg tagcggcgca
    13201 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    13261 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    13321 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    13381 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
    13441 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    13501 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    13561 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    13621 ttaacgctta caattt
//
