LOCUS       sequence-257679-        8958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of  RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Noeparast A, Giron P, Noor A, Bahadur Shahi R, De Brakeleer S, 
            Eggermont C, Vandenplas H, Boeckx B, Lambrechts D, De Greve J, 
            Teugels E
  TITLE     CRAF mutations in lung cancer can be oncogenic and predict 
            sensitivity to combined type II RAF and MEK inhibition.
  JOURNAL   Oncogene. 2019 Aug;38(31):5933-5941. doi: 10.1038/s41388-019-0866-7.
            Epub 2019 Jul 8.
  PUBMED    31285551
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0866-7"; journalName: "Oncogene"; date: 
            "2019-08"; volume: "38"; issue: "31"; pages: "5933-5941"
COMMENT     Sequence Label: pLenti-puro/RAF1-S259A
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1890..2093
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2044..2064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4166..4183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4206..4223)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4235..4255)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4240..4569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4420..4555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4579..5178
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4579..4598)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5075..5095
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5250..5483
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5555..5676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5592..5611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5646..5665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5716..5851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5871..5890)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5872..5890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5900..5917)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5900..5916)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5909..5931)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6058..6513
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6145..6164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6355..6376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6539..6643
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6644..6712,6713..7504)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6862..6881)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7675..8263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8164..8183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8417..8434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8551..8572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8587..8592,8593..8610,8611..8617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8625..8641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8630..8652
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8649..8665
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8649..8665
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8684..8704
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8686..8704
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8732..8958
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat aagcttggga gttccgctta cataacttac ggtaaatggc ccgcctggct
     1621 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1681 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1741 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1801 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1861 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1921 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1981 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2041 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     2101 tcagtgatag agatctccct atcagtgata gagatcgtcg actagtggat ccagtgtggt
     2161 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagaatgg agcacataca
     2221 gggagcttgg aagacgatca gcaatggttt tggattcaaa gatgccgtgt ttgatggctc
     2281 cagctgcatc tctcctacaa tagttcagca gtttggctat cagcgccggg catcagatga
     2341 tggcaaactc acagatcctt ctaagacaag caacactatc cgtgttttct tgccgaacaa
     2401 gcaaagaaca gtggtcaatg tgcgaaatgg aatgagcttg catgactgcc ttatgaaagc
     2461 actcaaggtg aggggcctgc aaccagagtg ctgtgcagtg ttcagacttc tccacgaaca
     2521 caaaggtaaa aaagcacgct tagattggaa tactgatgct gcgtctttga ttggagaaga
     2581 acttcaagta gatttcctgg atcatgttcc cctcacaaca cacaactttg ctcggaagac
     2641 gttcctgaag cttgccttct gtgacatctg tcagaaattc ctgctcaatg gatttcgatg
     2701 tcagacttgt ggctacaaat ttcatgagca ctgtagcacc aaagtaccta ctatgtgtgt
     2761 ggactggagt aacatcagac aactcttatt gtttccaaat tccactattg gtgatagtgg
     2821 agtcccagca ctaccttctt tgactatgcg tcgtatgcga gagtctgttt ccaggatgcc
     2881 tgttagttct cagcacagat attctacacc tcacgccttc acctttaaca cctccagtcc
     2941 ctcatctgaa ggttccctct cccagaggca gaggtcgaca gccacaccta atgtccacat
     3001 ggtcagcacc accctgcctg tggacagcag gatgattgag gatgcaattc gaagtcacag
     3061 cgaatcagcc tcaccttcag ccctgtccag tagccccaac aatctgagcc caacaggctg
     3121 gtcacagccg aaaacccccg tgccagcaca aagagagcgg gcaccagtat ctgggaccca
     3181 ggagaaaaac aaaattaggc ctcgtggaca gagagattca agctattatt gggaaataga
     3241 agccagtgaa gtgatgctgt ccactcggat tgggtcaggc tcttttggaa ctgtttataa
     3301 gggtaaatgg cacggagatg ttgcagtaaa gatcctaaag gttgtcgacc caaccccaga
     3361 gcaattccag gccttcagga atgaggtggc tgttctgcgc aaaacacggc atgtgaacat
     3421 tctgcttttc atggggtaca tgacaaagga caacctggca attgtgaccc agtggtgcga
     3481 gggcagcagc ctctacaaac acctgcatgt ccaggagacc aagtttcaga tgttccagct
     3541 aattgacatt gcccggcaga cggctcaggg aatggactat ttgcatgcaa agaacatcat
     3601 ccatagagac atgaaatcca acaatatatt tctccatgaa ggcttaacag tgaaaattgg
     3661 agattttggt ttggcaacag taaagtcacg ctggagtggt tctcagcagg ttgaacaacc
     3721 tactggctct gtcctctgga tggccccaga ggtgatccga atgcaggata acaacccatt
     3781 cagtttccag tcggatgtct actcctatgg catcgtattg tatgaactga tgacggggga
     3841 gcttccttat tctcacatca acaaccgaga tcagatcatc ttcatggtgg gccgaggata
     3901 tgcctcccca gatcttagta agctatataa gaactgcccc aaagcaatga agaggctggt
     3961 agctgactgt gtgaagaaag taaaggaaga gaggcctctt tttccccaga tcctgtcttc
     4021 cattgagctg ctccaacact ctctaccgaa gatcaaccgg agcgcttccg agccatcctt
     4081 gcatcgggca gcccacactg aggatatcaa tgcttgcacg ctgaccacgt ccccgaggct
     4141 gcctgtcttc tagacgcgta ccggtcatca tcaccatcac cattgagttt aaacctgctg
     4201 atcagcctcg actgtgcctt ctagtcgacc ctgtggaatg tgtgtcagtt agggtgtgga
     4261 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4321 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4381 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4441 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4501 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4561 ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4621 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4681 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4741 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4801 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4861 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4921 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4981 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5041 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5101 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5161 cgcaagcccg gtgcctgagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5221 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5281 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5341 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5401 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5461 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     5521 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     5581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5641 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     5701 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5761 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5821 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     5881 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5941 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6001 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6061 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6481 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6961 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7021 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7081 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7141 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7201 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7261 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7321 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7381 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7441 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7501 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7561 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7801 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8461 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8521 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8581 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8641 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     8701 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     8761 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     8821 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     8881 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     8941 ctagctcgat acataaac
//
