LOCUS       sequence-257696-        2729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human SON.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2729)
  TITLE     Allen Institute for Cell Sciences Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-82:SON-mEGFP
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cccgctcgcc ggaagtcggc ccaccgggac cggcgcggga ccagcgcggc accgcccctc
       61 actgccccgc cctacgacgg cttccctgcc ccattggtcc gcgcggccct gggtctccgc
      121 ggcgagaatc tagtctgatt ccctcccgcc ctgggcgctg cctgactgct ccccagccga
      181 gacacccaag ccttagcacg tttataatgt gacagtggta cggggtgcca cgcagtgttt
      241 ccaggagcgc cgggggggag cgccaaaatg ctaaacccat tcttagccaa ttgtgcgccg
      301 ctcacgagtc agagctgagc agctaccgcg gaaaaatctc gtgacacgcc tgactcctga
      361 tgagactaaa atgcaccggg ctttgaatgc cggtcgctta ggacatccgg cctattgcta
      421 aaaaaaaaaa tgaggctggt tatagaacgc actggggaag acacagggca acagcgagtt
      481 cctcctctca catgccagtg ggggcggggg ctgaaaaatt ctctcccgga tgcatatccc
      541 tgtcgaaact gaacttggcg ccacctgaga gcgcctgaaa gccgtgtgtt aaacaccggg
      601 ttgcccttct tgctcttctt cctcttcgcc tttaatctca ggcacttagc aagcctgtta
      661 aggctcccaa acactgctgg ggactgacaa cttatgcgga cacggtcgcc tcaagagaga
      721 cggctcctgt aatggcgcct gcgtcagaca ctaacatggc ggacatagtc gtgcgcggcg
      781 caaacgtcag cacctctacc ccagccgctc agaaacagca gaggagagca gggccgtgct
      841 cactgattgg tccctcggtg gagcgggccg gaccgggcat tccctttgga gccaatcagg
      901 aaggcgagag accgaattgg ccgctgcgcc tcctcccgag gcatgctggg agcctggagg
      961 actagcgagg aggagttgag agaacggagc ggacgccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagcg cgacaaacat
     1741 cgagcagatc tttaggtctt tcgtcgtcag taaattccgg gaaattcaac aggagctttc
     1801 caggtaaacg cctcccagat tcttctgctc ccaacggacc gttaggccct cacctagcct
     1861 tctttggcac ctttcttcgg agacgcagtc gttccccggc tcaggccccg ctagggcccc
     1921 gcctgggcct gggatccatt ttccgggccc cccccaaccg cccccccagc cgctcccttc
     1981 cttttccctc gaggaacttc gccttttccg aggtaaccca agactccctg tttcagaacc
     2041 gagttaagat gttcaccctt ctcccctgtt tgggtccccc ctgccgttta gctactcgta
     2101 ggcccggttg tccgccaggg acgggcctag ttccttcctc agctcctcct ccgccgccgc
     2161 gtatcccctc cccctctccg cctccgcgga ttttgccggc cggtgcctat gaccggaaag
     2221 ggtctctaac ggccgtttct tggttaatct taaatcctct ttattattgc gagcttcaga
     2281 ccgcttgggg gtctggatct attaataaga ttattcgggg cctagtgtcc tgtagtatct
     2341 gtcagccgtt ctgatagtta aatgtttcaa ttaaaacgaa ctcaaactga tgcacttgct
     2401 agtttgttaa aatactcgag ataaagatgg aaggggactt tgaaatggtt gcgattaatt
     2461 acagcaagtg aaagtggact ttgtgtgtag gtctattatt ttcttgctac ccctgcccca
     2521 gttgacacgc tatttttgtc attgctggta gactttacat aatttgcgct tggcttcaca
     2581 aaatttgaag gcgaagagat tgttaaagaa atggttgttt acttaggaaa atagtgtgtc
     2641 atgttcagcg gttgtctctc gatatttgct tttgtataag atactcattt ctttaaagta
     2701 cagaagtagg aaagagatct tgaaacgta
//
