LOCUS       sequence-25801-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, 
            Vassena R, Bilic J, Pekarik V, Tiscornia G, Edel M, Boue S, Belmonte
            JC
  TITLE     Efficient and rapid generation of induced pluripotent stem cells 
            from human keratinocytes.
  JOURNAL   Nat Biotechnol. 2008 Nov . 26(11):1276-84.
  PUBMED    18931654
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Nov . 26(11):1276-84."
COMMENT     Sequence Label: pMSCV-Flag-cMyc T58A
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggaatgatc tgccgccgcc atggactaca aggacgacga tgacaagctc gagcccctca
       61 acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag ccgtatttct
      121 actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg cagcccccgg
      181 cgcccagcga ggatatctgg aagaaattcg agctgctgcc cgcgccgccc ctgtccccta
      241 gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc tcccttcggg
      301 gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag atggtgaccg
      361 agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac gacgagacct
      421 tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc gccgccaagc
      481 tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc
      541 ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat ctgagcgccg
      601 ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac gacagcagct
      661 cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg gattctctgc
      721 tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc catgaggaga
      781 caccgccca
//
