LOCUS       sequence-25802-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, 
            Vassena R, Bilic J, Pekarik V, Tiscornia G, Edel M, Boue S, Belmonte
            JC
  TITLE     Efficient and rapid generation of induced pluripotent stem cells 
            from human keratinocytes.
  JOURNAL   Nat Biotechnol. 2008 Nov . 26(11):1276-84.
  PUBMED    18931654
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Nov . 26(11):1276-84."
COMMENT     Sequence Label: pMSCVpuro-Flag-cMyc T58A
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attgccgccg ccatggacta caaggacgac gatgacaagc tcgagcccct caacgttagc
       61 ttcaccaaca ggaactatga cctcgactac gactcggtgc agccgtattt ctactgcgac
      121 gaggaggaga acttctacca gcagcagcag cagagcgagc tgcagccccc ggcgcccagc
      181 gaggatatct ggaagaaatt cgagctgctg cccgcgccgc ccctgtcccc tagccgccgc
      241 tccgggctct gctcgccctc ctacgttgcg gtcacaccct tctcccttcg gggagacaac
      301 gacggcggtg gcgggagctt ctccacggcc gaccagctgg agatggtgac cgagctgctg
      361 ggaggagaca tggtgaacca gagtttcatc tgcgacccgg acgacgagac cttcatcaaa
      421 aacatcatca tccaggactg tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca
      481 gagaagctgg cctcctacca ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc
      541 ggccacagcg tctgctccac ctccagcttg tacctgcagg atctgagcgc cgccgcctca
      601 gagtgcatcg acccctcggt ggtcttcccc taccctctca acgacagcag ctcgcccaag
      661 tcctgcgcct cgcaagactc cagcgccttc tctccgtcct cggatctggt g
//
