LOCUS       sequence-258127-        6845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 7_pAAV-ProB10-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1289..1298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1295..2221
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2225..2227,2228..2230,2231..2944)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2225..2227,2228..2230,2231..2941)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2270..2291)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2531..2550)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2878..2899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2972..3560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3025..3045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3443..3454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3592..4068
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3791..3810)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4108..4248
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4108..4237
                     /label=AAV2 ITR
     rep_origin      4323..4778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4410..4429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4620..4641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4795..4814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4914..4936
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4974..4992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5060..5164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5165..5233,5234..6025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5383..5402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6196..6784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6685..6704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcttctg cagtgtgtgg gggttggtgg ggggaggggg
      181 ggcgggaggg cgggggactc gtttctgcca cctgcctgtg agaagaaaag ctgtaacccc
      241 agataaaagt tttcaccctt gtctctgagg atatcagctc ctctgctgtt gtcctgccag
      301 aaacagtgta tgggggtgag ggcttccgag ccacacacca ccctcttctt ccagaccttg
      361 ccttctgcat ggggagacac aagctgttta cagcaggagg ggattggtga tcagcccaca
      421 gggcaggatg ttggctgtga gcagctgcca aaagggaact ctctgggccc tgtggaatca
      481 gaagctttca ggtctagaaa ggccaccctt tcggcctttc gtctctcaag ctgtgtctag
      541 gcagaggccc aagttttggc caggaagtgt tagtgaccct atgacctact caaaaggggg
      601 ttcctgggag aggccaagtt gtgcttgtcc tcatcccagt tccccaactt tctgcccaca
      661 tgggagatgg acagactgat tgttctaaga tagacacccg gggcagtact gattatcccc
      721 agtgctagac ccagcaggaa ccaggggggc tttttctgcc acacacaaag ttcagttcag
      781 ctgaggtctt tgggctttca ttgcttaaaa gttaaagacc aagatggaag ggattggaat
      841 ctttggacac aggttccttg gttggccttc gctatgtact tatgggccga gagtcacctt
      901 agggatatat gtcccagact tcaagctggc ttcagtgtcc ttaggggtta acaagagaag
      961 ctaagtggat caagcctgtg ctgcagactt tgttgggagg ttggtgctgt gcccaccggg
     1021 gtaggaagca ggcaggcggc gtgggacagc caatcagagg aggagggagc tgcagaggct
     1081 ctaactgacc ctgatgcagc tgagccgtcc ttggagggtg ggagcccagg ggagaggagg
     1141 ggaggggcgg gggtggagaa gaacattcca cagagacaaa gtagggtcaa agaaaaaggg
     1201 ggaaggaacc aatcaggaag ccagatgaca gcaggagcat caagagaagc ctgtttctgt
     1261 gtgaggaccc ttgcctggag atggatccgc caccatggat tatggaggcg ccctgagtgc
     1321 cgttgggcgc gagctgctat ttgtaacgaa cccagtagtc gtcaatggct ctgtacttgt
     1381 gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa acggtgccca
     1441 aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac tgcttatgtt
     1501 ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg tgtgcgctat
     1561 cgagatggtc aaggtgattc tcgagttctt cttcgagttt aagaacccgt ccatgctgta
     1621 tctagccaca ggccaccgcg tccagtggtt gcgttacgcc gagtggcttc tcacctgccc
     1681 ggtcatttgc attcacctgt caaacctgac gggcttgtcc aacgactaca gcaggcgcac
     1741 catgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt ccgccatggc
     1801 caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg ctaacacgtt
     1861 ctttcacgct gccaaggcct acatcgaggg ttaccacacc gtgccgaagg gccggtgtcg
     1921 ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt tccccatcct
     1981 gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg tacggctcca ccgtcggcca
     2041 caccatcatt gacctgatgt cgaagaactg ctggggtctg ctcggccact acctgcgcgt
     2101 gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca aattgaacat
     2161 tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg ctggcgcggt
     2221 acccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2281 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2341 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2401 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2461 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2521 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2581 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2641 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2701 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2761 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2821 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2881 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2941 gtgataagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3001 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3061 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3121 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3181 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3241 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3301 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3361 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     3421 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3481 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3541 cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat
     3601 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     3661 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     3721 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     3781 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     3841 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     3901 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     3961 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4021 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag
     4081 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     4141 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4201 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     4261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     4321 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4381 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4501 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     4561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4621 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     4681 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     4741 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     4801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4981 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6841 catgt
//
