LOCUS       sequence-25813-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Gong L, Yeh ET
  TITLE     Identification of the activating and conjugating enzymes of the 
            NEDD8 conjugation pathway.
  JOURNAL   J Biol Chem. 1999 Apr 23;274(17):12036-42.
  PUBMED    10207026
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1999-04-23"; volume: "274(17)"; pages: "12036-42"
COMMENT     Sequence Label: pcDNA3/RGS-UBC12
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..675
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgtgggnntt ncgagctctc tggctactga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct tggatgatca agctgttctc gctgaagcag
      121 cagaagaagg aggaggagtc ggcgggcggc accaagggca gcagcaagaa ggcgtcggcg
      181 gcgcagctgc ggatccagaa ggacataaac gagctgaacc tgcccaagac gtgtgatatc
      241 agcttctcag atccagacga cctcctcaac ttcaagctgg tcatctgtcc tgatgagggc
      301 ttctacaaga gtgggaagtt tgtgttcagt tttaaggtgg gccagggtta cccgcatgat
      361 ccccccaagg tgaagtgtga gacaatggtc tatcacccca acattgacct cgagggcaac
      421 gtctgcctca acatcctcag agaggactgg aagccagtcc ttacgataaa ctccataatt
      481 tatggcctgc agtatctctt cttggagccc aaccccgagg acccactgaa caaggaggcc
      541 gcagaggtcc tgcagaacaa ccggcggctg tttgagcaga acgtgcagcg ctccatgcgg
      601 ggtggctaca tcggctccac ctactttgag cgctgcctga aatggtaccg aggatcacat
      661 caccatcacc atcactgtcn nttttaaann ggnccccccn nntntnntnn anttctnnan
      721 attccntcan nntggnggcc gctcnancat gcntctnnag ggncctnttc tatantgtcn
      781 cctang
//
