LOCUS       sequence-258200-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-3XKD
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             658..687
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    706..730
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8342..8366)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8415..8434)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8416..8434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8708..8829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8745..8764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8799..8818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8836..9291)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8973..8994)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9185..9204
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9318..9422
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9424..9781
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9424..9615
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9447..9467)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9632..9767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9816..10610
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9816..10610
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9870..9889)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10480..10499
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10798..10817)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10842..10889
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11218..11806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11707..11726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      601 gcggccgcca tggagcagaa actcatctct gaagaggatc tgagcccggg cggatccgag
      661 cagaaactca tctctgaaga ggatctgctg caggaattcg atatcacaag tttgtacaaa
      721 aaagcaggct ccgaattcgc ccttatggct agtggcagct gtcaggggtg cgaagaggac
      781 gaggaaactc tgaagaagtt gatagtcagg ctgaacaatg tccaggaagg aaaacagata
      841 gaaacgctgg tccaaatcct ggaggatctg ctggtgttca cgtactccga gcacgcctcc
      901 aagttatttc aaggcaaaaa tatccatgtg cctctgttga tcgtcttgga ctcctatatg
      961 agagtcgcga gtgtgcagca ggtgggttgg tcacttctgt gcaaattaat agaagtctgt
     1021 ccaggtacaa tgcaaagctt aatgggaccc caggatgttg gaaatgattg ggaagtcctt
     1081 ggtgttcacc aattgattct taaaatgcta acagttcata atgccagtgt aaacttgtca
     1141 gtgattggac tgaagacctt agatctcctc ctaacttcag gtaaaatcac cttgctgata
     1201 ttggatgaag aaagtgatat tttcatgtta atttttgatg ccatgcactc atttccagcc
     1261 aatgatgaag tccagaaact tggatgcaaa gctttacatg tgctgtttga gagagtctca
     1321 gaggagcaac tgactgaatt tgttgagaac aaagattata tgatattgtt aagtgcgtta
     1381 acaaatttta aagatgaaga ggaaattgtg cttcatgtgc tgcattgttt acattcccta
     1441 gcgattcctt gcaataatgt ggaagtcctc atgagtggca atgtcaggtg ttataatatt
     1501 gtggtggaag ctatgaaagc attccctatg agtgaaagaa ttcaagaagt gagttgctgt
     1561 ttgctccata ggcttacatt aggtaatttt ttcaatatcc tggtattaaa cgaagtccat
     1621 gagtttgtgg tgaaagctgt gcagcagtac ccagagaatg cagcattgca gatctcagcg
     1681 ctcagctgtt tggccctcct cactgagact attttcttaa atcaagattt agaggaaaag
     1741 aatgagaatc aagagaatga tgatgagggg gaagaagata aattgttttg gctggaagcc
     1801 tgttacaaag cattaacgtg gcatagaaag aacaagcacg tgcaggaggc cgcatgctgg
     1861 gcactaaata atctccttat gtaccaaaac agtttacatg agaagattgg agatgaagat
     1921 ggccatttcc cagctcatag ggaagtgatg ctctccatgc tgatgcattc ttcatcaaag
     1981 gaagttttcc aggcatctgc gaatgcattg tcaactctct tagaacaaaa tgttaatttc
     2041 agaaaaatac tgttatcaaa aggaatacac ctgaatgttt tggagttaat gcagaagcat
     2101 atacattctc ccgaagtggc tgaaagtggc tgtaaaatgc taaatcatct ttttgaagga
     2161 agcaacactt ccctggatat aatggcagca gtggtcccca aaatactaac agttatgaaa
     2221 cgtcatgaga catcattacc agtgcagctg gaggcgcttc gagctatttt acattttata
     2281 gtgcctggca tgccagaaga atccagggag gatacagaat ttcatcataa gctaaatatg
     2341 gttaaaaaac agtgtttcaa gaatgatatt cacaaactgg tcctagcagc tttgaacagg
     2401 ttcattggaa atcctgggat tcagaaatgt ggattaaaag taatttcttc tattgtacat
     2461 tttcctgatg cattagagat gttatccctg gaaggtgcta tggattcagt gcttcacaca
     2521 ctgcagatgt atccagatga ccaagaaatt cagtgtctgg gtttaagtct tataggatac
     2581 ttgattacaa agaagaatgt gttcatagga actggacatc tgctggcaaa aattctggtt
     2641 tccagcttat accgatttaa ggatgttgct gaaatacaga ctaaaggatt tcagacaatc
     2701 ttagcaatcc tcaaattgtc agcatctttt tctaagctgc tggtgcatca ttcatttgac
     2761 ttagtaatat tccatcaaat gtcttccaat atcatggaac aaaaggatca acagtttcta
     2821 aacctctgtt gcaagtgttt tgcaaaagta gctatggatg attacttaaa aaatgtgatg
     2881 ctagagagag cgtgtgatca gaataacagc atcatggttg aatgcttgct tctattggga
     2941 gcagatgcca atcaagcaaa ggagggatct tctttaattt gtcaggtatg tgagaaagag
     3001 agcagtccca aattggtgga actcttactg aatagtggat ctcgtgaaca agatgtacga
     3061 aaagcgttga cgataagcat tgggaaaggt gacagccaga tcatcagctt gctcttaagg
     3121 aggctggccc tggatgttgc caacaatagc atttgccttg gaggattttg tataggaaaa
     3181 gttgaacctt cttggcttgg tcctttattt ccagataaga cttctaattt aaggaaacaa
     3241 acaaatatag catctacact agcaagaatg gtgatcagat atcagatgaa aagtgctgtg
     3301 gaagaaggaa cagcctcagg cagcgatgga aatttttctg aagatgtgct gtctaaattt
     3361 gatgaatgga cctttattcc tgactcttct atggacagtg tgtttgctca aagtgatgac
     3421 ctggatagtg aaggaagtga aggctcattt cttgtgaaaa agaaatctaa ttcaattagt
     3481 gtaggagaat tttaccgaga tgccgtatta cagcgttgct caccaaattt gcaaagacat
     3541 tccaattcct tggggcccat ttttgatcat gaagatttac tgaagcgaaa aagaaaaata
     3601 ttatcttcag atgattcact caggtcatca aaacttcaat cccatatgag gcattcagac
     3661 agcatttctt ctctggcttc tgagagagaa tatattacat cactagacct ttcagcaaat
     3721 gaactaagag atattgatgc cctaagccag aaatgctgta taagtgttca tttggagcat
     3781 cttgaaaagc tggagcttca ccagaatgca ctcacgagct ttccacaaca gctatgtgaa
     3841 actctgaaga gtttgacaca tttggacttg cacagtaata aatttacatc atttccttct
     3901 tatttgttga aaatgagttg tattgctaat cttgatgtct ctcgaaatga cattggaccc
     3961 tcagtggttt tagatcctac agtgaaatgt ccaactctga aacagtttaa cctgtcatat
     4021 aaccagctgt cttttgtacc tgagaacctc actgatgtgg tagagaaact ggagcagctc
     4081 attttagaag gaaataaaat atcaggggta tgctccccct tgagactgaa ggaactgaag
     4141 attttaaacc ttagtaagaa ccacatttca tccctatcag agaactttct tgaggcttgt
     4201 cctaaagtgg agagtttcag tgccagaatg aattttcttg ctgctatgcc tttcttgcct
     4261 ccttctatga caatcctaaa attatctcag aacaaatttt cctgtattcc agaagcaatt
     4321 ttaaatcttc cacacttgcg gtctttagat atgagcagca atgatattca gtacctacca
     4381 ggtcccgcac actggaaatc tttgaactta agggaactct tatttagcca taatcagatc
     4441 agcatcttgg acttgagtga aaaagcatat ttatggtcta gagtagagaa actgcatctt
     4501 tctcacaata aactgaaaga gattcctcct gagattggct gtcttgaaaa tctgacatct
     4561 ctggatgtca gttacaactt ggaactaaga tcctttccca atgaaatggg gaaattaagc
     4621 aaaatatggg atcttccttt ggatgaactg catcttaact ttgattttaa acatatagga
     4681 tgtaaagcca aagacatcat aaggtttctt caacagcgat taaaaaaggc tgtgccttat
     4741 aaccgaatga aacttatgat tgtgggaaat actgggagtg gtaaaaccac cttattgcag
     4801 caattaatga aaaccaagaa atcagatctt ggaatgcaaa gtgccacagt tggcatagat
     4861 gtgaaagact ggcctatcca aataagagac aaaagaaaga gagatctcgt cctaaatgtg
     4921 tgggattttg caggtcgtga ggaattctat agtactcatc cccattttat gacgcagcga
     4981 gcattgtacc ttgctgtcta tgacctcagc aagggacagg ctgaagttga tgccatgaag
     5041 ccttggctct tcaatataaa ggctcgcgct tcttcttccc ctgtgattct cgttggcaca
     5101 catttggatg tttctgatga gaagcaacgc aaagcctgca tgagtaaaat caccaaggaa
     5161 ctcctgaata agcgagggtt ccctgccata cgagattacc actttgtgaa tgccaccgag
     5221 gaatctgatg ctttggcaaa acttcggaaa accatcataa acgagagcct taatttcaag
     5281 atccgagatc agcttgttgt tggacagctg attccagact gctatgtaga acttgaaaaa
     5341 atcattttat cggagcgtaa aaatgtgcca attgaatttc ccgtaattga ccggaaacga
     5401 ttattacaac tagtgagaga aaatcagctg cagttagatg aaaatgagct tcctcacgca
     5461 gttcactttc taaatgaatc aggagtcctt cttcattttc aagacccagc actgcagtta
     5521 agtgacttgt actttgtgga acccaagtgg ctttgtaaaa tcatggcaca gattttgaca
     5581 gtgaaagtgg aaggttgtcc aaaacaccct aagggaatta tttcgcgtag agatgtggaa
     5641 aaatttcttt caaaaaaaag gaaatttcca aagaactaca tgtcacagta ttttaagctc
     5701 ctagaaaaat tccagattgc tttgccaata ggagaagaat atttgctggt tccaagcagt
     5761 ttgtctgacc acaggcctgt gatagagctt ccccattgtg agaactctga aattatcatc
     5821 cgactatatg aaatgcctta ttttccaatg ggattttggt caagattaat caatcgatta
     5881 cttgagattt caccttacat gctttcaggg agagaacgag cacttcgccc aaacagaatg
     5941 tattggcgac aaggcattta cttaaattgg tctcctgaag cttattgtct ggtaggatct
     6001 gaagtcttag acaatcatcc agagagtttc ttaaaaatta cagttccttc ttgtagaaaa
     6061 ggctgtattc ttttgggcca agttgtggac cacattgatt ctctcatgga agaatggttt
     6121 cctgggttgc tggagattga tatttgtggt gaaggagaaa ctctgttgaa gaaatgggca
     6181 ttatatagtt ttaatgatgg cgaagaacat caaaaaatct tacttgatga cttgatgaag
     6241 aaagcagagg aaggagatct cttagtaaat ccagatcaac caaggctcac cattccaata
     6301 tctcagattg cccctgactt gattttggct gacctgccta gaaatattat gttgaataat
     6361 gatgagttgg aatttgaaca agctccagag tttctcctag gtgatggcag ttttggatca
     6421 gtttaccgag cagcctatga aggagaagaa gtggctgtgg caatttttaa taaacataca
     6481 tcactcaggc tgttaagaca agagcttgtg gtgctttgcc acctccacca ccccagtttg
     6541 atatctttgc tggcagctgg gattcgtccc cggatgttgg tgatggagtt agcctccaag
     6601 ggttccttgg atcgcctgct tcagcaggac aaagccagcc tcactagaac cctacagcac
     6661 aggattgcac tccacgtagc tgatggtttg agatacctcc actcagccat gattatatac
     6721 cgagcactga aaccccacaa tgtgctgctt ttcacactgt atcccaatgc tgccatcatt
     6781 gcaaagattg ctgcatacgg cattgctcag tactgctgta gaatggggat aaaaacatca
     6841 gagggcacac cagggtttcg tgcgcctgaa gttgccagag gaaatgtcat ttataaccaa
     6901 caggctgatg tttattcatt tggtttacta ctctatgaca ttttgacaac tggaggtaga
     6961 atagtagagg gtttgaagtt tccaaatgag tttgatgaat tagaaataca aggaaaatta
     7021 cctgatccag ttaaagaata tggttgtgcc ccatggccta tggttgagaa attaattaaa
     7081 cagtgtttga aagaaaatcc tcaagaaagg cctacttctg cccaggtctt tgacattttg
     7141 aattcagctg aattagtctg tctgacgaga cgcattttat tacctaaaaa cgtaattgtt
     7201 gaatgcatgg ttgctacaca tcacaacagc aggaatgcaa gcatttggct gggctgtggg
     7261 cacaccgaca gaggacagct ctcatttctt gacttaaata ctgaaggata cacttctgag
     7321 gaagttgctg atagtagaat attgtgctta gccttggtgc atcttcctgt tgaaaaggaa
     7381 agctggattg tgtctgggac acagtctggt actctcctgg tcatcaatac cgaagatggg
     7441 aaaaagagac ataccctaga aaagatgact gattctgtca cttgtttgta ttgcaattcc
     7501 ttttccaagc aaagcaaaca aaaaaatttt cttttggttg gaaccgctga tggcaagtta
     7561 gcaatttttg aagataagac tgttaagctt aaaggagctg ctcctttgaa gatactaaat
     7621 ataggaaatg tcagtactcc attgatgtgt ttgagtgaat ccacaaattc aacggaaaga
     7681 aatgtaatgt ggggaggatg tggcacaaag attttctcct tttctaatga tttcaccatt
     7741 cagaaactca ttgagacaag aacaagccaa ctgttttctt atgcagcttt cagtgattcc
     7801 aacatcataa cagtggtggt agacactgct ctctatattg ctaagcaaaa tagccctgtt
     7861 gtggaagtgt gggataagaa aactgaaaaa ctctgtggac taatagactg cgtgcacttt
     7921 ttaagggagg taatggtaaa agaaaacaag gaatcaaaac acaaaatgtc ttattctggg
     7981 agagtgaaaa ccctctgcct tcagaagaac actgctcttt ggataggaac tggaggaggc
     8041 catattttac tcctggatct ttcaactcgt cgacttatac gtgtaattta caacttttgt
     8101 aattcggtca gagtcatgat gacagcacag ctaggaagcc ttaaaaatgt catgctggta
     8161 ttgggctaca accggaaaaa tactgaaggt acacaaaagc agaaagagat acaatcttgc
     8221 ttgaccgttt gggacatcaa tcttccacat gaagtgcaaa atttagaaaa acacattgaa
     8281 gtgagaaaag aattagctga aaaaatgaga cgaacatctg ttgagaaaaa gggcgaattc
     8341 gacccagctt tcttgtacaa agtggtgatc ggtaccttaa ttaattaagg taccaggtaa
     8401 gtgtacccaa ttcgccctat agtgagtcgt attacaattc actcgatcgc ccttcccaac
     8461 agttgcgcag cctgaatggc gaatggagat ccaattttta agtgtataat gtgttaaact
     8521 actgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct
     8581 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     8641 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8701 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8821 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8881 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8941 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     9001 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     9061 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     9121 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9181 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9241 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     9301 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9361 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9421 atcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     9481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9721 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9781 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     9841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     9901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     9961 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
    10021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    10081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    10141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    10201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    10261 aagccggtct tgtcgatcag gatgatctgg acgaagaaca tcaggggctc gcgccagccg
    10321 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
    10381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10501 ctgaagaact tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
    10621 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    10681 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10741 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
    10801 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
    10861 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10921 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    10981 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    11041 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
    11101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    11161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    11221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    11281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    11341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    11401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    11461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    11521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    11581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    11641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    11701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    11761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    11821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    11881 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
    11941 ttacggggtc attagttcat agcccatata tggagttccg
//
