LOCUS       sequence-258220-       11319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: SP-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..11319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(699..725,726..728,729..3212,3213..3218,3219..3284,
                     3285..3287,3288..4802)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4815..4835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4872..4896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4926..5075
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5100..5120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5550..5906
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5925..6494
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(6499..6523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6656..6675)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6700..6748
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6950..7378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7037..7056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7247..7268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7387..7407)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7392..7721
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7572..7707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7634..7653
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7788..8582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7842..7861)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8452..8471
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8758..8879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8795..8814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8849..8868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8928..8944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8928..8944)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8941..8963)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8952..8968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8976..8982,8983..9000,9001..9006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9021..9042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9159..9176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9330..9918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9410..9429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10089..10880,10881..10949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10712..10731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10950..11054)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11129..11148)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat ggacaagaag tactccattg
      721 ggctcgctat cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc
      781 cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca
      841 ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag
      901 cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta
      961 atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg
     1021 aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt
     1081 accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata
     1141 aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact
     1201 tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac
     1261 tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg
     1321 ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac
     1381 agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc
     1441 tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca
     1501 aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag
     1561 accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag
     1621 tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc
     1681 accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca
     1741 aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa
     1801 gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg
     1861 agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg
     1921 gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg
     1981 atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga
     2041 taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca
     2101 aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg
     2161 cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc
     2221 ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca
     2281 aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta
     2341 tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact
     2401 atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca
     2461 acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg
     2521 acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag
     2581 atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca
     2641 tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca
     2701 atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat
     2761 ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca
     2821 tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg
     2881 caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg
     2941 tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc
     3001 aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta
     3061 taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga
     3121 atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac
     3181 tggacatcaa tcggctctcc gactacgacg tggctgctat cgtgccccag tcttttctca
     3241 aagatgattc tattgataat aaagtgttga caagatccga taaagctaga gggaagagtg
     3301 ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga
     3361 acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc
     3421 tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca
     3481 ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca
     3541 aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa
     3601 aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct
     3661 acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat
     3721 ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg
     3781 aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga
     3841 ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag
     3901 aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt
     3961 ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg
     4021 aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc
     4081 ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca
     4141 aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa
     4201 tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata
     4261 aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa
     4321 acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac
     4381 tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt
     4441 ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg
     4501 agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg
     4561 ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa
     4621 acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg
     4681 acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga
     4741 ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag
     4801 acagcagggc tgaccccaag aagaagagga aggtgtcgcc agggatccgt cgacttgacg
     4861 cgttgatatc aacaagtttg tacaaaaaag caggctacaa agaggccagc ggttccggac
     4921 gggctgacgc attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt
     4981 ttgaccttga catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca
     5041 gtgacgccct tgatgatttc gacctggaca tgctgattaa ctctagaagt tccggatctc
     5101 cgaaaaagaa acgcaaagtt ggtagccagt acctgcccga caccgacgac cggcaccgga
     5161 tcgaggaaaa gcggaagcgg acctacgaga cattcaagag catcatgaag aagtccccct
     5221 tcagcggccc caccgaccct agacctccac ctagaagaat cgccgtgccc agcagatcca
     5281 gcgccagcgt gccaaaacct gccccccagc cttacccctt caccagcagc ctgagcacca
     5341 tcaactacga cgagttccct accatggtgt tccccagcgg ccagatctct caggcctctg
     5401 ctctggctcc agcccctcct caggtgctgc ctcaggctcc tgctcctgca ccagctccag
     5461 ccatggtgtc tgcactggct caggcaccag cacccgtgcc tgtgctggct cctggacctc
     5521 cacaggctgt ggctccacca gcccctaaac ctacacaggc cggcgagggc acactgtctg
     5581 aagctctgct gcagctgcag ttcgacgacg aggatctggg agccctgctg ggaaacagca
     5641 ccgatcctgc cgtgttcacc gacctggcca gcgtggacaa cagcgagttc cagcagctgc
     5701 tgaaccaggg catccctgtg gcccctcaca ccaccgagcc catgctgatg gaataccccg
     5761 aggccatcac ccggctcgtg acaggcgctc agaggcctcc tgatccagct cctgcccctc
     5821 tgggagcacc aggcctgcct aatggactgc tgtctggcga cgaggacttc agctctatcg
     5881 ccgatatgga tttctcagcc ttgctgggct ctggcagcgg cagccgggat tccagggaag
     5941 ggatgttttt gccgaagcct gaggccggct ccgctattag tgacgtgttt gagggccgcg
     6001 aggtgtgcca gccaaaacga atccggccat ttcatcctcc aggaagtcca tgggccaacc
     6061 gcccactccc cgccagcctc gcaccaacac caaccggtcc agtacatgag ccagtcgggt
     6121 cactgacccc ggcaccagtc cctcagccac tggatccagc gcccgcagtg actcccgagg
     6181 ccagtcacct gttggaggat cccgatgaag agacgagcca ggctgtcaaa gcccttcggg
     6241 agatggccga tactgtgatt ccccagaagg aagaggctgc aatctgtggc caaatggacc
     6301 tttcccatcc gcccccaagg ggccatctgg atgagctgac aaccacactt gagtccatga
     6361 ccgaggatct gaacctggac tcacccctga ccccggaatt gaacgagatt ctggatacct
     6421 tcctgaacga cgagtgcctc ttgcatgcca tgcatatcag cacaggactg tccatcttcg
     6481 acacatctct gttttgaaac ccagctttct tgtacaaagt ggttgatatc atctagagcg
     6541 gccgcaggta cctgaataac taaggccgct tccctttagt gagggttaat gcttcgagca
     6601 gacaaagggt tcgatcccta ccggttagta atgagtttaa acgggggagg ctaactgaaa
     6661 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     6721 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6781 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6841 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6901 ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt
     6961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7381 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7441 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7501 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7561 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7621 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7681 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7741 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7801 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7861 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7921 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7981 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8041 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8101 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     8161 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     8221 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     8281 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     8341 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8401 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8461 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8521 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8581 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8641 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8701 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8761 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8881 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    11101 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    11161 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
    11221 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
    11281 ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
